Description
Detailed protocols supporting DExCon study. From preparation of ssDNA for CRISPR mediated knock in; delivery of CRISPR/Cas9 as RNP via magnetofection and screening.Protocol for Spheroid invasion assay “on a chip" is included as well.
Date made available | 6 Dec 2021 |
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Publisher | University of Manchester Figshare |
Keywords
- Protocols
- CRISPR gene editing technology
- spheroids
- invasion analysis
- Single strand DNA
- Biological Techniques