Figure 3 components

  • Christopher Blanford (Creator)
  • Abubaker Mohamed (Creator)
  • Sam P. de Visser (Creator)
  • Yuhei Hayamizu (Creator)

Dataset

Description

Figure 3. Response of enzyme-functionalized GFETs to two nitriles. Figure 3a. Normalized response of wild-type QueF and QueF-Y5Y fusion protein to concentrations of preQ<sub>0</sub> to 500nM. Gray vertical lines represent approximate timings for analyte addition. Arrowed symbols: ● = sensor wash in phosphate buffer, ◆ = addition of 4 µM NADPH, ■ = addition of 1 M substrate. GFET conditions: <em>V</em><sub>g</sub>: 500 mV, <em>V</em><sub>d</sub>: 5 mV. Figure 3a. Normalized response of wild-type QueF and QueF-Y5Y fusion protein to concentrations benzyl cyanide (BnCN) to 500nM. Gray vertical lines represent approximate timings for analyte addition. Gray vertical lines represent approximate timings for analyte addition. Arrowed symbols: ● = sensor wash in phosphate buffer, ◆ = addition of 4 µM NADPH, ■ = addition of 1 M substrate. GFET conditions: <em>V</em><sub>g</sub>: 500 mV, <em>V</em><sub>d</sub>: 5 mV. Figure 3c. Normalized response curve fittings for each sample. Error bars ranges are mean ± standard deviation. Number of replicates are given in Table 1. Standard deviation in panel c is calculated from the pooled variance of all signals over 1 min.
Date made available31 Aug 2022
PublisherUniversity of Manchester figshare

Keywords

  • GFET sensors
  • GFET sensors functionalized
  • nitrile reductase
  • field effect transistor
  • benzyl cyanide

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