MATN2 knock-down human immortalized podocytes LC-MS/MS

Dataset

Description

Basement membranes (BMs) are ubiquitous extracellular matrices whose composition remains elusive, limiting our understanding of BM regulation and function. By developing a bioinformatic and in vivo discovery pipeline, we define a network of over 220 human proteins localized to BMs. More than 100 BM-network genes associate with human phenotypes and by screening over 63,000 germline genomic sequences from families with rare disorders, we discovered novel predicted loss-of-function variants in MATN2. Biochemical analyses suggest that MATN2 interacts with many BM proteins, and we found that depletion of MATN2 affected levels of core BM components in human podocyte-derived extracellular matrix.
Date made available2022
PublisherPRoteomics IDEntifications Database
Date of data production1 Aug 2022

Keywords

  • Q Exactive HF
  • podocyte
  • human
  • Shotgun mass spectrometry
  • MATN2

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