Proteomics analysis of the effect of Slf1 deletion on yeast response to oxidative stress via LC-MSMS

The mechanisms by which RNA-binding proteins control the translation of subsets of mRNAs are not yet clear. Slf1p is an atypical La motif containing protein (LARP). LARPs are members of a superfamily of RNA-binding proteins conserved across eukaryotes. Transcriptomics and RIP-Seq analysis suggested an effect of Slf1 on the amount of mRNA of genes involved in the response to oxidative stress. To quantify these effects at the protein level, we used label-free mass spectrometry to compare the proteomes of wild-type and slf1Δ mutant strains following oxidative stress conditions. This analysis identified several proteins which are normally induced in response to hydrogen peroxide, but where this increase is attenuated in the slf1Δ mutant.