HIDEMs and mesoangioblasts were stimulated with IFN-γ, TNF-α or IL-1β (20ng/ml) for 24h. Cells were trypsinized and washed, followed by surface staining for HLA-ABC, HLA-DR, CD40, PD-L1 or fluorochrome matched isotype controls and analysis by flow cytometry. Experiments were carried out in duplicates. n=4. Median fluorescence intensities of the markers were examined, and were shown as Mean ± SE.
|Date made available
|25 Jan 2013