Raw data for Figure 3C: Mesoangioblasts and HIDEMs do not interfer with T cell activation

Ou Li (Contributor)
Karen English (Contributor)
Rossana Tonlorenzi (Contributor)
Giulio Cossu (Contributor)
Francesco Saverio Tedesco (Contributor)
Kathryn J. Wood (Contributor)

Division of Cell Matrix Biology & Regenerative Medicine (L5)

Dataset

Description

CFSE labelled PBMCs (5 x 104/well) were stimulated with anti CD3/CD28 beads (1 x 104/well) (P+B) in the presence or absence of HIDEMs/mesoangioblasts at HIDEM/mesoangioblast:PBMC = 1:4 ratio. Cells were harvested on day 3, 4, 5 or 6 and analysed for CFSE dilution and expression of CD25 and CD69. The number of CD3+7AAD- cells expressing CD25 or CD69 using counting beads and the % of CD25+ and CD69+ cells were calculated from the data. Experiments were carried out in duplicates. n=2.

Date made available	25 Jan 2013
Publisher	figshare

DOI
10.6084/m9.figshare.106726

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1 Article

Human iPSC-derived mesoangioblasts, like their tissue-derived counterparts, suppress T cell proliferation through IDO- and PGE-2-dependent pathways
Li, O., English, K., Tonlorenzi, R., Cossu, G., Saverio Tedesco, F. & Wood, K. J., 2013, In: F1000Research. 2, p. 24
Research output: Contribution to journal › Article › peer-review

Raw data for Figure 3C: Mesoangioblasts and HIDEMs do not interfer with T cell activation

Description

DOI

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Research output

Human iPSC-derived mesoangioblasts, like their tissue-derived counterparts, suppress T cell proliferation through IDO- and PGE-2-dependent pathways

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