Star_Protocols_Vinter_fixed_dataset

  • Daisy Vinter (Creator)

Dataset

Description

Example datasets for fixed imaging from Vinter et al, Star Protocols 2021.
Expression_domain.ims: Imaging of a scFv-mNG-NLS x hbP2>Suntag-hb embryo in nc13 at expression domain level, in Imaris format. channels - 1: SunTag mRNA smFISH, 2: scFv-mNG, 3: DAPI. Microscopy settings as described in Vinter et al, Star Protocols 2021.
ng.tif and suntag.tif: High magnification imaging of a portion of the expression domain of a scFv-mNG-NLS x hbP2>Suntag-hb embryo, for calculation of ribosome number at translation sites. ng.tif is scFv-NG channel, suntag.tif is SunTag mRNA smFISH channel. Microscopy settings as described in Vinter et al, Star Protocols 2021.
Date made available6 Sept 2021
PublisherMendeley Data

Keywords

  • Microscopy
  • Confocal Microscopy

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