Surface analysis measurements of the adsorption of bilirubin oxidase from Myrothecium verrucaria



    Content type: Research data
    Research data type: Experimental data
    Created date: 2015-8-05
    Last modified date: 2015-11-07

    Abstract: Data recorded from: - an electrochemical quartz crystal microbalance (EQCMD, files ending in .qsd) using a Q-Sense E1 QCMD with a modified QEM401 flow cell (files readable in QTools) - a potentiostat (files ending in .ids) using an Ivium CompactStat (files readable in IviumSoft) - a Farfield dual polarization interferometer (DPI, files ending in .xpt) Processed data in Excel spreadsheets (.xlsx) and Origin Project Files (.opj). Data relate to the concentration-dependent adsorption of the enzyme bilirubin oxidase from Myrothecium verrucaria (MvBOx) on bare gold and silica surfaces, and those modified to provide a surface with a uniform charge or polarity.

    Table of contents: Surface modifications EQCMD - EQCMD: frequency, dissipation and current responses to the adsorption of MvBOx on gold sensor surfaces modified with short- and long-chain modifiers terminated with positive, negative or apolar groups. - DPI: phase and refractive index data for the same, but with silane-modified silicon oxynitride sensor surfaces increasing concentration 3MPA EQCMD - EQCMD - Concentration increase on 3MPA: raw and processed QCMD and electrochemical data for MvBOx adsorption on gold sensors modified with 3-mercaptopropionic acid (3MPA) for concentrations from 1 to 50 mg/ml - Concentration increase on gold: data as for previous but taken from unmodified (bare gold) QCM sensors - Concentartion increase BSA on SiOx: data as for previous but taken from bare silicon oxide coated QCM sensors - DPI: Excel file with combined processed data for MvBOx adsorption on bare DPI sensors from solutions from 0.1 to 15 mg/ml Viscosity measurements.xlsx: Falling ball viscometer measurements for MvBOx solutions and fits using Solver to Huggins and Kraemer models.
    Date made available3 Aug 2015
    PublisherUniversity of Manchester

    Research Beacons, Institutes and Platforms

    • Manchester Institute of Biotechnology
    • National Graphene Institute


    • DPI
    • E-QCM-D
    • QCM-D
    • bilirubin oxidase
    • laccase
    • multicopper oxidase
    • gold
    • silica
    • thiol
    • SAM
    • adsorption

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