X-ray phase microtomography with a single grating for high-throughput investigations of biological tissue

  • Marie-Christine Zdora (Creator)
  • Joan Vila-Comamala (Creator)
  • Georg Schulz (Creator)
  • Anna Khimchenko (Creator)
  • Alexander Hipp (Creator)
  • Andrew C. Cook (Creator)
  • Daniel Dilg (Creator)
  • Christian David (Creator)
  • Christian Grünzweig (Creator)
  • Christoph Rau (Creator)
  • Pierre Thibault (Creator)
  • Irene Zanette (Creator)



    The high-throughput 3D visualisation of biological specimens is essential for studying diseases and developmental disorders. It requires imaging methods that deliver high-contrast, high-resolution volumetric information at short sample preparation and acquisition times. Here we show that X-ray phase-contrast tomography using a single grating can provide a powerful alternative to commonly employed techniques, such as high-resolution episcopic microscopy (HREM). We present the phase tomography of a mouse embryo in paraffin obtained with an X-ray single-grating interferometer at I13-2 Beamline at Diamond Light Source and discuss the results in comparison with HREM measurements. The excellent contrast and quantitative density information achieved non-destructively and without staining using a simple, robust setup make X-ray single-grating interferometry an optimum candidate for high-throughput imaging of biological specimens as an alternative for existing methods like HREM.
    Date made available1 Feb 2017


    • high-throughput imaging
    • episcopic microscopy
    • X-ray single-grating interferometer
    • alternative
    • 13-2 Beamline
    • acquisition times
    • X-ray phase microtomography
    • sample preparation
    • X-ray phase-contrast tomography
    • X-ray single-grating interferometry
    • HREM measurements
    • high-throughput investigations
    • phase tomography
    • specimen
    • Diamond Light Source
    • mouse embryo
    • imaging methods
    • density information
    • high-throughput 3 D visualisation

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