Research output per year
Research output per year
- 29 results
Search results
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2025
Enabling structural biological electron paramagnetic resonance spectroscopy in membrane proteins through spin labelling
Shah, A., Wort, J. L., Ma, Y. & Pliotas, C., Feb 2025, In: Current Opinion in Chemical Biology. 84, 102564.Research output: Contribution to journal › Article › peer-review
Open Access -
The mechanosensitive channel YbiO has a conductance equivalent to the largest gated-pore
Lane, B. J., Dionysopoulou, M., Yan, N., Lippiat, J. D., Muench, S. P. & Pliotas, C., 3 Apr 2025, In: Structure. 33, p. 1-11Research output: Contribution to journal › Article › peer-review
Open AccessFile2 Downloads (Pure) -
2024
Conformational dynamics and asymmetry in multimodal inhibition of membrane-bound pyrophosphatases
Liu, J., Shah, A., Ma, Y., Hardman, K., Johansson, N., Ribeiro, O., Brookfield, A., Bowen, A., Yli-Kauhaluoma, J., Xhaard, H., Jeuken, L., Goldman, A., Pliotas, C. & Vidilaseris, K., 4 Dec 2024, In: eLife.Research output: Contribution to journal › Article › peer-review
Open Access -
Monitoring the Conformational Ensemble and Lipid Environment of a Mechanosensitive Channel Under Cyclodextrin-Induced Membrane Tension
Lane, B. J., Ma, Y., Yan, N., Wang, B., Ackermann, K., Karamanos, T. K., Bode, B. E. & Pliotas, C., 6 Jun 2024, In: Structure. 32, p. 1-12Research output: Contribution to journal › Article › peer-review
Open AccessFile24 Downloads (Pure) -
2023
Approaches for the modulation of mechanosensitive MscL channel pores
Lane, B. J. & Pliotas, C., 15 Mar 2023, In: Frontiers in Chemistry. 11, 1162412.Research output: Contribution to journal › Review article › peer-review
Open Access -
Darobactin B Stabilises a Lateral‐Closed Conformation of the BAM Complex in E. coli Cells
Haysom, S. F., Machin, J., Whitehouse, J. M., Horne, J. E., Fenn, K., Ma, Y., Mkami, H. E., Böhringer, N., Schäberle, T. F., Ranson, N. A., Radford, S. E. & Pliotas, C., 21 Aug 2023, In: Angewandte Chemie International Edition. 62, 34, e202218783.Research output: Contribution to journal › Article › peer-review
Open Access -
Membrane force reception: mechanosensation in G protein-coupled receptors and tools to address it
Hardman, K., Goldman, A. & Pliotas, C., 1 Oct 2023, In: Current Opinion in Physiology. 35, 100689.Research output: Contribution to journal › Review article › peer-review
Open Access -
2022
Genetic and cellular characterization of MscS-like putative channels in the filamentous fungus Aspergillus nidulans
Dionysopoulou, M., Yan, N., Wang, B., Pliotas, C. & Diallinas, G., 31 Dec 2022, In: Channels.Research output: Contribution to journal › Article › peer-review
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HDX-guided EPR spectroscopy to interrogate membrane protein dynamics
Lane, B. J., Wang, B., Ma, Y., Calabrese, A. N., Mkami, H. E. & Pliotas, C., Sept 2022, In: STAR protocols.Research output: Contribution to journal › Article › peer-review
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Novel variants provide differential stabilisation of human equilibrative nucleoside transporter 1 states
Boakes, J. C., Harborne, S. P. D., Ngo, J. T. S., Pliotas, C. & Goldman, A., 8 Nov 2022, In: Frontiers in Molecular Biosciences. 9, 970391.Research output: Contribution to journal › Article › peer-review
Open Access -
Pocket delipidation induced by membrane tension or modification leads to a structurally analogous mechanosensitive channel state
Wang, B., Lane, B. J., Kapsalis, C., Ault, J. R., Sobott, F., Mkami, H. E., Calabrese, A. N., Kalli, A. C. & Pliotas, C., 7 Apr 2022, In: Structure.Research output: Contribution to journal › Article › peer-review
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2021
Using pulsed EPR in the structural analysis of integral membrane proteins
Hartley, A. M., Ma, Y., Lane, B. J., Wang, B. & Pliotas, C., 16 Nov 2021, Electron Paramagnetic Resonance. Chechik, V., Murphy, D. M. & Bode, B. E. (eds.). Oxford: Royal Society of Chemistry, Vol. 27. p. 74-108 45 p. (Specialist Periodical Reports (SPR) - Electron Paramagnetic Resonance).Research output: Chapter in Book/Conference proceeding › Chapter › peer-review
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2020
In-lipid structure of pressure sensitive domains hints mechanosensitive channel functional diversity
Kapsalis, C., Ma, Y., Bode, B. E. & Pliotas, C., 21 Jul 2020, In: Biophysical Journal. 119, 2, p. 448-459 12 p.Research output: Contribution to journal › Article › peer-review
Open Access -
The tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex
Koo, C. Z., Harrison, N., Noy, P. J., Szyroka, J., Matthews, A. L., Hsia, H.-E., Müller, S. A., Tüshaus, J., Goulding, J., Willis, K., Apicella, C., Cragoe, B., Davis, E., Keles, M., Malinova, A., McFarlane, T. A., Morrison, P. R., Nguyen, H. T. H., Sykes, M. C. & Ahmed, H. & 11 others, , 4 Sept 2020, In: Journal of Biological Chemistry. 295, 36, p. 12822-12839 18 p.Research output: Contribution to journal › Article › peer-review
Open Access -
2019
Allosteric activation of an ion channel triggered by modification of mechanosensitive nano-pockets
Kapsalis, C., Wang, B., Mkami, H. E., Pitt, S. J., Schnell, J. R., Smith, T. K., Lippiat, J. D., Bode, B. E. & Pliotas, C., 10 Oct 2019, In: Nature Communications. 10, p. 1-14 14 p., 4619.Research output: Contribution to journal › Article › peer-review
Open Access -
Optimization of recombinant membrane protein production in the engineered Escherichia coli strains SuptoxD and SuptoxR
Michou, M., Kapsalis, C., Pliotas, C. & Skretas, G., 19 Jul 2019, In: ACS Synthetic Biology. 8, 7, p. 1631–1641 11 p.Research output: Contribution to journal › Article › peer-review
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2017
Adenosine monophosphate binding stabilizes the KTN domain of the Shewanella denitrificans Kef potassium efflux system
Pliotas, C., Grayer, S. C., Ekkerman, S., Chan, A. K. N., Healy, J., Marius, P., Bartlett, W., Khan, A., Cortopassi, W. A., Chandler, S. A., Rasmussen, T., Benesch, J. L. P., Paton, R. S., Claridge, T. D. W., Miller, S., Booth, I. R., Naismith, J. H. & Conway, S. J., 15 Aug 2017, In: Biochemistry. 56, 32, p. 4219–4234 16 p.Research output: Contribution to journal › Article › peer-review
Open Access -
Enhanced imaging of lipid rich nanoparticles embedded in methylcellulose films for transmission electron microscopy using mixtures of heavy metals
Asadi, J., Ferguson, S., Raja, H., Hacker, C., Marius, P., Ward, R., Pliotas, C., Naismith, J. H. & Lucocq, J., Aug 2017, In: Micron. 99, p. 40-48 9 p.Research output: Contribution to journal › Article › peer-review
Open Access -
Ion Channel Conformation and Oligomerization Assessment by Site-Directed Spin Labeling and Pulsed-EPR
Pliotas, C., 10 Aug 2017, A Structure-Function Toolbox for Membrane Transporter and Channels. Ziegler, C. (ed.). Cambridge, MA: Academic Press, Vol. 594. p. 203-242 40 p. (Methods in Enzymology).Research output: Chapter in Book/Conference proceeding › Chapter › peer-review
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Sparse labeling PELDOR spectroscopy on multimeric mechanosensitive membrane channels
Ackermann, K., Pliotas, C., Valera, S., Naismith, J. H. & Bode, B. E., 7 Nov 2017, In: Biophysical Journal. 113, 9, p. 1968-1978 11 p.Research output: Contribution to journal › Article › peer-review
Open Access -
Spectator no more, the role of the membrane in regulating ion channel function
Pliotas, C. & Naismith, J. H., Aug 2017, In: Current Opinion in Structural Biology. 45, p. 59-66 8 p.Research output: Contribution to journal › Article › peer-review
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2016
Accurate Extraction of Nanometer Distances in Multimers by Pulse EPR
Valera, S., Ackermann, K., Pliotas, C., Huang, H., Naismith, J. H. & Bode, B. E., 24 Mar 2016, In: Chemistry - A European Journal. 22, 14, p. 4700-4703 4 p.Research output: Contribution to journal › Article › peer-review
Open Access -
Nanoparticle suspensions enclosed in methylcellulose: a new approach for quantifying nanoparticles in transmission electron microscopy
Hacker, C., Asadi, J., Pliotas, C., Ferguson, S. G. A., Sherry, L., Marius, P., Tello, J. A., Jackson, D., Naismith, J. H. & Lucocq, J., 4 May 2016, In: Scientific Reports. 6, p. 1-13 13 p., 25275.Research output: Contribution to journal › Article › peer-review
Open Access -
2015
The role of lipids in mechanosensation
Pliotas, C., Dahl, A. C. E., Rasmussen, T., Mahendran, K. R., Smith, T. K., Marius, P., Gault, J., Banda, T., Rasmussen, A., Miller, S., Robinson, C. V., Bayley, H., Sansom, M. S. P., Booth, I. R. & Naismith, J. H., Dec 2015, In: Nature Structural and Molecular Biology. 22, p. 991–998 8 p.Research output: Contribution to journal › Article › peer-review
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2014
Probing the structure of the mechanosensitive channel of small conductance in lipid bilayers with pulsed electron-electron double resonance
Ward, R., Pliotas, C., Branigan, E., Hacker, C., Rasmussen, A., Hagelueken, G., Booth, I. R., Miller, S., Lucocq, J., Naismith, J. H. & Schiemann, O., 18 Feb 2014, In: Biophysical Journal. 106, 4, p. 834-842 9 p.Research output: Contribution to journal › Article › peer-review
Open Access -
Understanding the structural requirements for activators of the Kef bacterial potassium efflux system
Healy, J., Ekkerman, S., Pliotas, C., Richard, M., Bartlett, W., Grayer, S. C., Morris, G. M., Miller, S., Booth, I. R., Conway, S. J. & Rasmussen, T., 1 Apr 2014, In: Biochemistry. 53, 12, p. 1982–1992 11 p.Research output: Contribution to journal › Article › peer-review
Open Access -
2013
Quantification of free cysteines in membrane and soluble proteins using a fluorescent dye and thermal unfolding
Branigan, E., Pliotas, C., Hageluken, G. & Naismith, J. H., Nov 2013, In: Nature protocols. 8, p. 2090–2097 8 p.Research output: Contribution to journal › Article › peer-review
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2012
Conformational state of the MscS mechanosensitive channel in solution revealed by pulsed electron-electron double resonance (PELDOR) spectroscopy
Pliotas, C., Ward, R. J., Branigan, E., Rasmussen, A., Hageluken, G., Huang, H., Black, S. S., Booth, I. R., Schiemann, O. & Naismith, J. H., 10 Sept 2012, In: Proceedings of the National Academy of Sciences of the United States of America. 109, 40, p. E2675-E2682 8 p.Research output: Contribution to journal › Article › peer-review
Open Access -
2010
Mechanism of ligand-gated potassium efflux in bacterial pathogens
Roosild, T. P., Castronovo, S., Healy, J., Miller, S., Pliotas, C., Rasmussen, T., Bartlett, W., Conway, S. J. & Booth, I. R., 16 Nov 2010, In: Proceedings of the National Academy of Sciences of the United States of America. 107, 46, p. 19784-19789 6 p.Research output: Contribution to journal › Article › peer-review
Open Access