Research output per year
Research output per year
- 1 - 50 out of 159 results
Search results
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2025
Engineered enzymes for enantioselective nucleophilic aromatic substitutions
Lister, T. M., Roberts, G. W., Hossack, E. J., Zhao, F., Burke, A. J., Johannissen, L. O., Hardy, F. J., Millman, A. A. V., Leys, D., Larrosa, I. & Green, A. P., 13 Mar 2025, In: Nature. 639, p. 375–381Research output: Contribution to journal › Article › peer-review
Open Access -
Expression and purification of Mycobacterium tuberculosis F420-dependent glucose-6-phosphate dehydrogenase enzyme using Escherichia coli
Aderemi, A. V., Snee, M., Tunnicliffe, R. B., Golovanova, M., Cain, K. M., Munro, A. W., Waltho, J. P. & Leys, D., 1 Apr 2025, In: Protein Expression and Purification. 228, 106650.Research output: Contribution to journal › Article › peer-review
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2024
Photocobilins integrate B12 and bilin photochemistry for enzyme control
Zhang, S., Jeffreys, L. N., Poddar, H., Yu, Y., Liu, C., Patel, K., Johannissen, L. O., Zhu, L., Cliff, M. J., Yan, C., Schirò, G., Weik, M., Sakuma, M., Levy, C. W., Leys, D., Heyes, D. J. & Scrutton, N. S., Dec 2024, In: Nature Communications. 15, 1, 2740.Research output: Contribution to journal › Article › peer-review
Open Access -
The prFMNH2-binding chaperone LpdD assists UbiD decarboxylase activation
Gahloth, D., Fisher, K., Marshall, S. & Leys, D., 1 Feb 2024, In: Journal of Biological Chemistry. 300, 2, 105653.Research output: Contribution to journal › Article › peer-review
Open Access -
2023
Efficient NADPH-dependent dehalogenation afforded by a self-sufficient reductive dehalogenase
Fisher, K., Halliwell, T., Payne, K., Ragala, G., Hay, S., Rigby, S. & Leys, D., 1 Sept 2023, In: Journal of Biological Chemistry. 299, 9, 105086.Research output: Contribution to journal › Article › peer-review
Open Access -
High‐titer Bio‐Styrene Production Afforded by Whole‐cell Cascade Biotransformation
Messiha, H., Scrutton, N. & Leys, D., 4 Jan 2023, (E-pub ahead of print) In: ChemCatChem.Research output: Contribution to journal › Article › peer-review
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Redox driven B12-ligand switch drives CarH photoresponse
Poddar, H., Rios-Santacruz, R., Heyes, D. J., Shanmugam, M., Brookfield, A., Johannissen, L. O., Levy, C. W., Jeffreys, L. N., Zhang, S., Sakuma, M., Colletier, J. P., Hay, S., Schirò, G., Weik, M., Scrutton, N. S. & Leys, D., 21 Aug 2023, In: Nature Communications. 14, 1, 5082.Research output: Contribution to journal › Article › peer-review
Open Access -
Stability Engineering of Ferulic Acid Decarboxylase Unlocks Enhanced Aromatic Acid Decarboxylation.
Roberts, G. W., Fisher, K., Jowitt, T. & Leys, D., 2023, In: Current Research in Chemical Biology. 3, 100043.Research output: Contribution to journal › Article › peer-review
Open Access -
Structure Based Discovery of Inhibitors of CYP125 and CYP142 from Mycobacterium tuberculosis
Katariya, M. M., Snee, M., Tunnicliffe, R. B., Kavanagh, M. E., Boshoff, H. I. M., Amadi, C. N., Levy, C. W., Munro, A. W., Abell, C., Leys, D., Coyne, A. G. & McLean, K. J., 22 May 2023, In: Chemistry: A European Journal . 29, 29, e202203868.Research output: Contribution to journal › Article › peer-review
Open Access -
2022
A guide to time-resolved structural analysis of light-activated proteins
Poddar, H., Heyes, D., Schirò, G., Weik , M., Leys, D. & Scrutton, N., 1 Feb 2022, In: The FEBS Journal. 289, 3, p. 576-595 20 p.Research output: Contribution to journal › Article › peer-review
Open Access -
A new strategy for hit generation: Novel in cellulo active inhibitors of CYP121A1 from Mycobacterium tuberculosis via a combined X-ray crystallographic and phenotypic screening approach (XP screen)
Frederickson, M., Selvam, I. R., Evangelopoulos, D., McLean, K. J., Katariya, M. M., Tunnicliffe, R. B., Campbell, B., Kavanagh, M. E., Charoensutthivarakul, S., Blankley, R. T., Levy, C. W., de Carvalho, L. P. S., Leys, D., Munro, A. W., Coyne, A. G. & Abell, C., 15 Feb 2022, In: European Journal of Medicinal Chemistry. 230, 114105.Research output: Contribution to journal › Article › peer-review
Open Access -
A Vitamin B2-Photocatalysed Approach to Methionine Analogues
Knowles, O. J., Johannissen, L. O., Crisenza, G. E. M., Hay, S., Leys, D. & Procter, D. J., 5 Dec 2022, In: Angewandte Chemie. International Edition. 61, 50Research output: Contribution to journal › Article › peer-review
Open Access -
Structural and biochemical characterisation of the prenylated flavin mononucleotide-dependent indole-3-carboxylic acid decarboxylase
Gahloth, D., Fisher, K., Payne, K. A. P., Cliff, M., Levy, C. & Leys, D., 24 Feb 2022, In: Journal of Biological Chemistry. p. 101771Research output: Contribution to journal › Article › peer-review
Open Access -
Structural insights into UbiD reversible decarboxylation
Roberts, G. W. & Leys, D., 17 Jun 2022, (Accepted/In press) In: Current Opinion in Structural Biology.Research output: Contribution to journal › Article › peer-review
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2021
Directed evolution of prenylated FMN-dependent Fdc supports efficient in vivo isobutene production
Saaret, A., Villiers, B., Stricher, F., Anissimova, M., Cadillon, M., Spiess, R., Hay, S. & Leys, D., 6 Sept 2021, In: Nature Communications.Research output: Contribution to journal › Article › peer-review
Open Access -
Heterologous expression of cobalamin dependent class-III enzymes
Halliwell, T., Fisher, K., Payne, K., Rigby, S. E. J. & Leys, D., 1 Jan 2021, In: Protein Expression and Purification. 177, p. 105743 105743.Research output: Contribution to journal › Article › peer-review
Open Access -
Structure and mechanism of Pseudomonas aeruginosa PA0254/HudA, a prFMNdependent pyrrole-2-carboxylic acid decarboxylase linked to virulence
Payne, K., Marshall, S., Fisher, K., Rigby, S., Cliff, M., Spiess, R., Cannas, D., Larrosa, I., Hay, S. & Leys, D., 5 Feb 2021, (Accepted/In press) In: ACS Catalysis.Research output: Contribution to journal › Article › peer-review
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Structure and Mechanism of Pseudomonas aeruginosa PA0254/HudA, a prFMN-Dependent Pyrrole-2-carboxylic Acid Decarboxylase Linked to Virulence
Payne, K. A. P., Marshall, S. A., Fisher, K., Rigby, S. E. J., Cliff, M. J., Spiess, R., Cannas, D. M., Larrosa, I., Hay, S. & Leys, D., 5 Mar 2021, In: ACS Catalysis. 11, 5, p. 2865-2878 14 p.Research output: Contribution to journal › Article › peer-review
Open Access -
Synthetic enzyme‐catalysed CO2 fixation reactions
Aleku, G. A., Roberts, G. W., Titchiner, G. R. & Leys, D., 25 Feb 2021, In: Chemsuschem.Research output: Contribution to journal › Article › peer-review
Open AccessFile38 Downloads (Pure) -
The In Vitro Production of prFMN for Reconstitution of UbiD Enzymes
Marshall, S. A., Fisher, K. & Leys, D., 23 Mar 2021, In: Methods in molecular biology (Clifton, N.J.). 2280, p. 219-227 9 p.Research output: Contribution to journal › Article › peer-review
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UbiD domain dynamics underpins aromatic decarboxylation
Marshall, S., Payne, K., Fisher, K., Titchiner, G., Levy, C., Hay, S. & Leys, D., 20 Aug 2021, In: Nature Communications.Research output: Contribution to journal › Article › peer-review
Open Access -
2020
A biological route to conjugated alkenes: microbial production of hepta-1,3,5-triene
Messiha, H., Payne, K., Scrutton, N. & Leys, D., 10 Dec 2020, (Accepted/In press) In: ACS Synthetic Biology.Research output: Contribution to journal › Article › peer-review
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Biocatalytic reduction of α,β-unsaturated carboxylic acids to allylic alcohols
Aleku, G., Roberts, G. & Leys, D., 24 Apr 2020, (Accepted/In press) In: Green Chemistry.Research output: Contribution to journal › Article › peer-review
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Catabolic Reductive Dehalogenase Substrate Complex Structures Underpin Rational Repurposing of Substrate Scope
Halliwell, T., Fisher, K., Payne, K. A. P., Rigby, S. E. J. & Leys, D., 2 Sept 2020, In: Microorganisms. 8, 9Research output: Contribution to journal › Article › peer-review
Open Access -
Enzymatic C-H activation of aromatic compounds through CO2 fixation
Aleku, G., Saaret, A., Bradshaw Allen, R., Derrington, S., Titchiner, G., Gostimskaya, I., Gahloth, D., Parker, D. A., Hay, S. & Leys, D., 1 Nov 2020, In: Nature chemical biology. 16, 11, p. 1255-1260 6 p.Research output: Contribution to journal › Article › peer-review
Open AccessFile252 Downloads (Pure) -
Ferulic acid decarboxylase (Fdc1) controls oxidative maturation of the prFMN cofactor
Balaikaite, A., Chisanga, M., Fisher, K., Heyes, D., Spiess, R. & Leys, D., 25 Aug 2020, (Accepted/In press) In: ACS chemical biology.Research output: Contribution to journal › Article › peer-review
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Ferulic Acid Decarboxylase Controls Oxidative Maturation of the Prenylated Flavin Mononucleotide Cofactor
Balaikaite, A., Chisanga, M., Fisher, K., Heyes, D. J., Spiess, R. & Leys, D., 18 Sept 2020, In: ACS chemical biology. 15, 9, p. 2466-2475 10 p.Research output: Contribution to journal › Article › peer-review
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Flavin doesn’t put all oxygens in one basket
Leys, D. & Scrutton, N. S., 22 Apr 2020, In: Nature chemical biology. 16, 5, p. 485-486Research output: Contribution to journal › Article
Open AccessFile130 Downloads (Pure) -
2019
A brain-permeable inhibitor of the neurodegenerative disease target kynurenine 3-monooxygenase prevents accumulation of neurotoxic metabolites
Zhang, S., Sakuma, M., Deora, G. S., Levy, C., Klausing, A., Breda, C., Read, K. D., Ross, B. P., Wright Muelas, M., Day, P., O'Hagan, S., Kell, D., Schwarcz, R., Leys, D., Heyes, D., Giorgini, F. & Scrutton, N., 2019, In: Communications Biology.Research output: Contribution to journal › Article › peer-review
Open Access -
Enzymatic Carboxylation of 2-Furoic Acid Yields 2,5-Furandicarboxylic Acid (FDCA)
Payne, K. A. P., Marshall, S., Fisher, K., Cliff, M. J., Cannas, D., Yan, C., Heyes, D. J., Parker, D., Larrosa, I. & Leys, D., 15 Feb 2019, In: ACS Catalysis. 9, 4, p. 2854-2865 12 p.Research output: Contribution to journal › Article › peer-review
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Enzymatic control of cycloadduct conformation ensures reversible 1,3 dipolar cycloaddition in a prFMN dependent decarboxylase
Bailey, S., Payne, K., Saaret, A., Marshall, S., Gostimskaya, I., Kosov, I., Fisher, K., Hay, S. & Leys, D., 16 Sept 2019, In: Nature Chemistry. 11, p. 1049-1057Research output: Contribution to journal › Article › peer-review
Open AccessFile50 Downloads (Pure) -
Novel insights into P450 BM3 interactions with FDA-approved antifungal azole drugs
Jeffreys, L., Poddar, H., Golovanova, M., Levy, C., Girvan, H., Mclean, K., Voice, M. W., Leys, D. & Munro, A., 2019, In: Nature Scientific Reports. 9, 1, p. 1-12 12 p., 1577.Research output: Contribution to journal › Article › peer-review
Open Access -
Structural basis for enzymatic photocatalysis in chlorophyll biosynthesis
Zhang, S., Heyes, D. J., Feng, L., Sun, W., Johannissen, L. O., Liu, H., Levy, C. W., Li, X., Yang, J., Yu, X., Lin, M., Hardman, S. J. O., Hoeven, R., Sakuma, M., Hay, S., Leys, D., Rao, Z., Zhou, A., Cheng, Q. & Scrutton, N. S., 23 Oct 2019, In: Nature. 574, p. 722-725Research output: Contribution to journal › Article › peer-review
Open AccessFile586 Downloads (Pure) -
Structure of the Cannabis sativa olivetol-producing enzyme reveals cyclization plasticity in type III polyketide synthases
Kearsey, L. J., Prandi, N., Karuppiah, V., Yan, C., Leys, D., Toogood, H., Takano, E. & Scrutton, N. S., 2019, In: FEBS Journal.Research output: Contribution to journal › Article › peer-review
Open Access -
The UbiX flavin prenyltransferase reaction mechanism resembles Class I terpene cyclase chemistry
Marshall, S., Payne, K., Fisher, K., White, M., Ni Cheallaigh, A., Balaikaite, A., Rigby, S. & Leys, D., 29 May 2019, In: Nature Communications.Research output: Contribution to journal › Article › peer-review
Open Access -
2018
Characterisation of a putrescine transaminase from Pseudomonas putida and its application to the synthesis of benzylamine derivatives
Galman, J., Gahloth, D., Parmeggiani, F., Slabu, I. & Leys, D., 2018, In: Frontiers in Bioengineering and Biotechnology.Research output: Contribution to journal › Article › peer-review
Open Access -
Characterization of a Putrescine Transaminase From Pseudomonas putida and its Application to the Synthesis of Benzylamine Derivatives
Turner, N., Galman, J., Slabu, I., Gahloth, D., Parmeggiani, F. & Leys, D., 21 Dec 2018, In: Frontiers in Bioengineering and Biotechnology. 6, p. 205Research output: Contribution to journal › Article › peer-review
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Flavin metamorphosis: cofactor transformation through prenylation
Leys, D., 2018, In: Current Opinion in Chemical Biology. 47, p. 117-125Research output: Contribution to journal › Article › peer-review
Open Access -
Heterologous Production and Purification of a Functional Chloroform Reductive Dehalogenase
Jugder, B. E., Payne, K. A. P., Fisher, K., Bohl, S., Lebhar, H., Manefield, M., Lee, M., Leys, D. & Marquis, C. P., 16 Mar 2018, In: ACS chemical biology. 13, 3, p. 548-552 5 p.Research output: Contribution to journal › Article › peer-review
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NADPH-Driven Organohalide Reduction by a Nonrespiratory Reductive Dehalogenase
Collins, F. A., Fisher, K., Payne, K. A. P., Gaytan Mondragon, S., Rigby, S. E. J. & Leys, D., 26 Jun 2018, In: Biochemistry. 57, 25, p. 3493-3502Research output: Contribution to journal › Article › peer-review
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P450-Catalyzed Regio- and Diastereoselective Steroid Hydroxylation: Efficient Directed Evolution Enabled by Mutability Landscaping
Acevedo-Rocha, C. G., Gamble, C., Lonsdale, R., Li, A., Nett, N., Hoebenreich, S., Lingnau, J. B., Wirtz, C., Fares, C., Hinrichs, H., Deege, A., Mulholland, A. J., Nov, Y., Leys, D., Mclean, K., Munro, A. & Reetz, M. T., 6 Apr 2018, In: ACS Catalysis. 8, 4, p. 3395-3410 16 p.Research output: Contribution to journal › Article › peer-review
Open Access13 Downloads (Pure) -
Structural and Catalytic Properties of the Peroxygenase P450 enzyme CYP152K6 from Bacillus methanolicus
Girvan, H., Poddar, H., Mclean, K., Nelson, D. R., Hollywood, K., Levy, C., Leys, D. & Munro, A., Nov 2018, In: Journal of Inorganic Biochemistry. 188Research output: Contribution to journal › Article › peer-review
Open Access -
Terminal Alkenes from Acrylic Acid Derivatives via Non-Oxidative Enzymatic Decarboxylation by Ferulic Acid Decarboxylases
Aleku, G. A., Prause, C., Bradshaw-Allen, R. T., Plasch, K., Glueck, S. M., Bailey, S. S., Payne, K. A. P., Parker, D. A., Faber, K. & Leys, D., 17 Jul 2018, In: ChemCatChem. 10, 17, p. 3736-3745 10 p.Research output: Contribution to journal › Article › peer-review
Open Access -
The role of conserved residues in Fdc decarboxylase in prenylated flavin mononucleotide oxidative maturation, cofactor isomerization, and catalysis
Bailey, S. S., Payne, K. A. P., Fisher, K., Marshall, S. A., Cliff, M. J., Spiess, R., Parker, D. A., Rigby, S. E. J. & Leys, D., 16 Feb 2018, In: Journal of Biological Chemistry. 293, 7Research output: Contribution to journal › Article › peer-review
Open Access -
2017
A common mechanism for coenzyme cobalamin-dependent reductive dehalogenases
Johannissen, L., Leys, D. & Hay, S., 2017, In: Physical Chemistry Chemical Physics. 19, 8, p. 6090-6094 5 p.Research output: Contribution to journal › Article › peer-review
Open Access -
Adenylation Activity of Carboxylic Acid Reductases Enables the Synthesis of Amides
Wood, A., Weise, N., Frampton, J. D., Dunstan, M., Hollas, M., Derrington, S., Lloyd, R. C., Quaglia, D., Parmeggiani, F., Leys, D., Turner, N. & Flitsch, S., 13 Nov 2017, In: Angewandte Chemie. 56, 46, p. 14498-14501 4 p.Research output: Contribution to journal › Article › peer-review
Open AccessFile82 Downloads (Pure) -
Production of alkenes and novel secondary products by P450 OleTJE using novel H2O2-generating fusion protein systems
Matthews, S., Tee, K. L., Rattray, N., Mclean, K., Leys, D., Parker, D., Blankley, R. & Munro, A., Mar 2017, In: FEBS Letters. 591, 5, p. 737-750 14 p.Research output: Contribution to journal › Article › peer-review
Open AccessFile88 Downloads (Pure) -
Structural basis of catalysis in the bacterial monoterpene synthases linalool synthase and 1,8-cineole synthase
Karuppiah, V., Ranaghan, K. E., Leferink, N., Johannissen, L., Shanmugam, M., Ni Cheallaigh, A., Bennett, N. J., Takano, E., Gardiner, J., van der Kamp, M. W., Hay, S., Mulholland, A. J., Leys, D. & Scrutton, N., 2017, In: ACS Catalysis.Research output: Contribution to journal › Article › peer-review
Open Access -
Structural insights into the ene-‐reductase synthesis of Profens
Waller, J., Toogood, H., Karuppiah, V., Rattray, N., Mansell, D., Leys, D., Gardiner, J., Fryszkowska, A., Ahmed, S. T., Bandichhor, R., Reddy, G. P. & Scrutton, N., 2017, In: Organic and Biomolecular Chemistry.Research output: Contribution to journal › Article › peer-review
Open Access -
Structures of carboxylic acid reductase reveal domain dynamics underlying catalysis
Gahloth, D., Dunstan, M. S., Quaglia, D., Klumbys, E., Lockhart-Cairns, M. P., Hill, A., Derrington, S. R., Scrutton, N. S., Turner, N. J. & Leys, D., 2017, In: Nature chemical biology. 13, p. 975-981 7 p.Research output: Contribution to journal › Article › peer-review
Open AccessFile204 Downloads (Pure)