Derren Heyes

2024

Photocobilins integrate B₁₂ and bilin photochemistry for enzyme control

Zhang, S., Jeffreys, L. N., Poddar, H., Yu, Y., Liu, C., Patel, K., Johannissen, L. O., Zhu, L., Cliff, M. J., Yan, C., Schirò, G., Weik, M., Sakuma, M., Levy, C. W., Leys, D., Heyes, D. J. & Scrutton, N. S., Dec 2024, In: Nature Communications. 15, 1, 2740.

Research output: Contribution to journal › Article › peer-review

Open Access

Probing Ferryl Reactivity in a Nonheme Iron Oxygenase Using an Expanded Genetic Code

Hardy, F. J., Quesne, M. G., Gérard, E. F., Zhao, J., Ortmayer, M., Taylor, C. J., Ali, H. J., Slater, J. W., Levy, C. W., Heyes, D. J., Bollinger, J. M., De visser, S. P. & Green, A. P., 2 Aug 2024, In: ACS Catalysis . 14, 15, p. 11584-11590 7 p.

Research output: Contribution to journal › Article › peer-review

Open Access

2023

How is substrate halogenation triggered by the vanadium haloperoxidase from Curvularia inaequalis?

Gerard, E., Mokkawes, T., Johannissen, L., Warwicker, J., Spiess, R., Blanford, C. F., Hay, S., Heyes, D. & De Visser, S., 6 Jun 2023, In: ACS Catalysis. 13, 12

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Open Access

Photoinduced Electron Transfer from a 1,4,5,6-Tetrahydro Nicotinamide Adenine Dinucleotide (Phosphate) Analog to Oxidized Flavin in an Ene-Reductase Flavoenzyme

Speirs, M., Hardman, S., Iorgu, A. I., Johannissen, L., Heyes, D., Scrutton, N., Sazanovich, I. V. & Hay, S., 6 Apr 2023, In: Journal of Physical Chemistry Letters. 14, 13, p. 3236-3242

Research output: Contribution to journal › Article › peer-review

Open Access

Redox driven B₁₂-ligand switch drives CarH photoresponse

Poddar, H., Rios-Santacruz, R., Heyes, D. J., Shanmugam, M., Brookfield, A., Johannissen, L. O., Levy, C. W., Jeffreys, L. N., Zhang, S., Sakuma, M., Colletier, J. P., Hay, S., Schirò, G., Weik, M., Scrutton, N. S. & Leys, D., Dec 2023, In: Nature Communications. 14, 1, 5082.

Research output: Contribution to journal › Article › peer-review

Open Access

2022

A Designed Photoenzyme for Enantioselective [2+2]-Cycloadditions

Trimble, J., Crawshaw, R., Hardy, F., Levy, C., Brown, M. J. B., Fuerst, D. E., Heyes, D., Obexer, R. & Green, A., 21 Sept 2022, In: Nature.. 611, 7937

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Open Access

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A guide to time-resolved structural analysis of light-activated proteins

Poddar, H., Heyes, D., Schirò, G., Weik , M., Leys, D. & Scrutton, N., 1 Feb 2022, In: The FEBS Journal. 289, 3, p. 576-595 20 p.

Research output: Contribution to journal › Article › peer-review

Open Access

How Photoactivation Triggers Protochlorophyllide Reduction: Computational Evidence of a Stepwise Hydride Transfer During Chlorophyll Biosynthesis

Johannissen, L., Taylor, A., Hardman, S., Heyes, D., Scrutton, N. & Hay, S., 8 Mar 2022, (Accepted/In press) In: ACS Catalysis.

Research output: Contribution to journal › Article › peer-review

Single crystal spectroscopy and multiple structures from one crystal (MSOX) define catalysis in copper nitrite reductases

Rose, S. L., Baba, S., Okumura, H., Antonyuk, S. V., Sasaki, D., Hedison, T. M., Shanmugam, M., Heyes, D. J., Scrutton, N. S., Kumasaka, T., Tosha, T., Eady, R. R., Yamamoto, M. & Hasnain, S. S., 26 Jul 2022, In: Proceedings of the National Academy of Sciences of the United States of America. 119, 30, e2205664119.

Research output: Contribution to journal › Article › peer-review

Open Access

Solution-State Inter-Copper Distribution of Redox Partner-Linked Copper Nitrite Reductases: A Pulsed Electron–Electron Double Resonance Spectroscopy Study

Hedison, T. M., Iorgu, A. I., Calabrese, D., Heyes, D. J., Shanmugam, M. & Scrutton, N. S., 22 Jul 2022, In: The journal of physical chemistry letters. p. 6927-6934

Research output: Contribution to journal › Article › peer-review

Open Access

2021

A Noncanonical Tryptophan Analogue Reveals an Active Site Hydrogen Bond Controlling Ferryl Reactivity in a Heme Peroxidase

Ortmayer, M., Hardy, F. J., Quesne, M. G., Fisher, K., Levy, C., Heyes, D. J., Catlow, C. R. A., Visser, S. P. D., Rigby, S. E. J., Hay, S. & Green, A. P., 26 Jul 2021, In: JACS Au.

Research output: Contribution to journal › Article › peer-review

A non-canonical tryptophan analogue reveals an active site hydrogen bond controlling ferryl reactivity in a heme peroxidase

Ortmayer, M., Hardy, F., Quesne, M., Fisher, K., Levy, C., Heyes, D., Catlow, C. R. A., De Visser, S., Rigby, S., Hay, S. & Green, A., 23 Apr 2021, (Accepted/In press) In: JACS Au.

Research output: Contribution to journal › Article › peer-review

Interplay Between Chromophore-Binding and Domain Assembly by the B12-Dependent Photoreceptor Protein, CarH

Camacho, I., Black, R., Heyes, D., Johannissen, L. O., Ramakers, L. A. I., Bellina, B., Barran, P., Hay, S. & Jones, A. R., 5 May 2021, In: Chemical Science.

Research output: Contribution to journal › Article › peer-review

Open Access

Making molecules with photodecarboxylases: a great start or a false dawn?

Hedison, T., Heyes, D. & Scrutton, N., 14 Dec 2021, (Accepted/In press) In: Current Research in Chemical Biology.

Research output: Contribution to journal › Article › peer-review

2020

Active Intermediates in Copper Nitrite Reductase Reactions Probed by a Cryotrapping‐Electron Paramagnetic Resonance Approach

Hedison, T. M., Shanmugam, M., Heyes, D. J., Edge, R. & Scrutton, N., 30 Apr 2020, (E-pub ahead of print) In: Angewandte Chemie International Edition.

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Open Access

Advantages of brain penetrating inhibitors of kynurenine-3-monooxygenase for treatment of neurodegenerative diseases

Zhang, S., Collier, M. E. W., Heyes, D., Giorgini, F. & Scrutton, N., 1 Dec 2020, (E-pub ahead of print) In: Archives of Biochemistry and Biophysics.

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Dual Role of the Active Site ‘Lid’ Regions of Protochlorophyllide Oxidoreductase in Photocatalysis and Plant Development

Zhang, S., Godwin, A., Taylor, A., Hardman, S., Jowitt, T., Johannissen, L., Hay, S., Baldock, C., Heyes, D. & Scrutton, N., 31 Aug 2020, (E-pub ahead of print) In: The FEBS Journal.

Research output: Contribution to journal › Article › peer-review

Open Access

Ferulic acid decarboxylase (Fdc1) controls oxidative maturation of the prFMN cofactor

Balaikaite, A., Chisanga, M., Fisher, K., Heyes, D., Spiess, R. & Leys, D., 25 Aug 2020, (Accepted/In press) In: ACS chemical biology.

Research output: Contribution to journal › Article › peer-review

Ferulic Acid Decarboxylase Controls Oxidative Maturation of the Prenylated Flavin Mononucleotide Cofactor

Balaikaite, A., Chisanga, M., Fisher, K., Heyes, D. J., Spiess, R. & Leys, D., 18 Sept 2020, In: ACS chemical biology. 15, 9, p. 2466-2475 10 p.

Research output: Contribution to journal › Article › peer-review

Insights into the H2O2 ‐driven catalytic mechanism of fungal lytic polysaccharide monooxygenases

Hedison, T. M., Breslmayr, E., Shanmugam, M., Karnpakdee, K., Heyes, D. J., Green, A. P., Ludwig, R., Scrutton, N. S. & Kracher, D., 23 Dec 2020, In: The FEBS Journal.

Research output: Contribution to journal › Article › peer-review

Open Access

Low Carbon Strategies for Sustainable Bio-alkane Gas Production and Renewable Energy

Amer, M., Wojcik, E., Sun, C., Hoeven, R., Hughes, J., Faulkner, M., Yunus, I. S., Tait, S., Johannissen, L., Hardman, S., Heyes, D., Chen, G.-Q., Smith, M. H., Jones, P. R., Toogood, H. & Scrutton, N., 19 May 2020, In: Energy & Environmental Science.

Research output: Contribution to journal › Article › peer-review

Open Access

Photochemical mechanism of light-driven fatty acid photodecarboxylase

Heyes, D. J., Lakavath, B., Hardman, S. J. O., Sakuma, M., Hedison, T. M. & Scrutton, N. S., 19 Jun 2020, In: ACS Catalysis. 10, 12, p. 6691-6696 6 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Photocycle of cyanobacteriochrome TePixJ

Hardman, S. J. O., Heyes, D. J., Sazanovich, I. V. & Scrutton, N. S., 18 Aug 2020, In: Biochemistry. 59, 32, p. 2909-2915 7 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Pressure and temperature effects on formation of aminoacrylate intermediates of tyrosine phenol-lyase demonstrate reaction dynamics

Phillips, R. S., Craig, S., Kovalevsky, A. Y., Gerlits, O. O., Weiss, K. L., Iorgu, A. I., Heyes, D. J. & Hay, S., 2020, In: ACS Catalysis.

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Protein Conformational Change Is Essential for Reductive Activation of Lytic Polysaccharide Monooxygenase by Cellobiose Dehydrogenase

Breslmayr, E., Laurent, C. V. F. P., Scheiblbrandner, S., Jerkovic, A., Heyes, D. J., Oostenbrink, C., Ludwig, R., Hedison, T. M., Scrutton, N. S. & Kracher, D., 9 Apr 2020, In: ACS Catalysis. p. 4842-4853

Research output: Contribution to journal › Article › peer-review

Open Access

Radical-based photoinactivation of fatty acid photodecarboxylases

Lakavath, B., Hedison, T., Heyes, D., Shanmugam, M., Sakuma, M., Hoeven, R., Tilakaratna, V. & Scrutton, N., 26 Apr 2020, In: Analytical Biochemistry|Anal Biochem. 600, 113749.

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Open Access

Rewiring the ‘Push-Pull’ Catalytic Machinery of a Heme Enzyme using an Expanded Genetic Code

Ortmayer, M., Fisher, K., Basran, J., Wolde-Michael, E. M., Heyes, D. J., Levy, C., Lovelock, S. L., Anderson, J. L. R., Raven, E. L., Hay, S., Rigby, S. E. J. & Green, A. P., 2020, In: ACS Catalysis. 10, 4, p. 2735-2746 12 p.

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The catalytic mechanism of aromatic nitration by cytochrome P450 TxtE: Involvement of a ferric-peroxynitrite intermediate

Louka, S., Barry, S. M., Heyes, D. J., Mubarak, M. Q. E., Ali, H. S., Alkhalaf, L., Munro, A. W., Scrutton, N. S., Challis, G. L. & De Visser, S. P., 19 Aug 2020, (E-pub ahead of print) In: Journal of the American Chemical Society.

Research output: Contribution to journal › Article › peer-review

Ultrafast Vibrational Energy Transfer between Protein and Cofactor in a Flavoenzyme

Hardman, S. J. O., Iorgu, A. I., Heyes, D. J., Scrutton, N. S., Sazanovich, I. V. & Hay, S., 4 Jun 2020, (E-pub ahead of print) In: The Journal of Physical Chemistry B.

Research output: Contribution to journal › Article › peer-review

Open Access

2019

A brain-permeable inhibitor of the neurodegenerative disease target kynurenine 3-monooxygenase prevents accumulation of neurotoxic metabolites

Zhang, S., Sakuma, M., Deora, G. S., Levy, C., Klausing, A., Breda, C., Read, K. D., Ross, B. P., Wright Muelas, M., Day, P., O'Hagan, S., Kell, D., Schwarcz, R., Leys, D., Heyes, D., Giorgini, F. & Scrutton, N., 2019, In: Communications Biology.

Research output: Contribution to journal › Article › peer-review

Open Access

Enzymatic Carboxylation of 2-Furoic Acid Yields 2,5-Furandicarboxylic Acid (FDCA)

Payne, K. A. P., Marshall, S., Fisher, K., Cliff, M. J., Cannas, D., Yan, C., Heyes, D. J., Parker, D., Larrosa, I. & Leys, D., 15 Feb 2019, In: ACS Catalysis. 9, 4, p. 2854-2865 12 p.

Research output: Contribution to journal › Article › peer-review

Kinetic characterisation of Erv1, a key component for protein import and folding in yeast mitochondria

Tang, X., Ang, S. K., Ceh Pavia, E., Heyes, D. & Lu, H. (Corresponding), 2019, In: The FEBS Journal.

Research output: Contribution to journal › Article › peer-review

Open Access

Light-induced structural changes in a full-length cyanobacterial phytochrome probed by time-resolved X-ray scattering

Heyes, D., Hardman, S., Pedersen, M. N., Woodhouse, J., De La Mora, E., Wulff, M., Weik, M., Cammarata, M., Scrutton, N. & Schiro, G., 2019, In: Communications Biology. 2, 1

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Open Access

Redox characterisation of Erv1, a key component for protein import and folding in yeast mitochondria: Electron flow mechanism of MIA40 pathway

Ceh Pavia, E., Tang, X., Liu, Y., Heyes, D., Zhao, B., Xiao, P. & Lu, H. (Corresponding), 2019, In: The FEBS Journal. FEBS15136.

Research output: Contribution to journal › Article › peer-review

Open Access

Solvent-slaved protein motions accompany proton coupled electron transfer reactions catalysed by copper nitrite reductase

Hedison, T., Heyes, D., Shanmugam, M., Iorgu, A. I. & Scrutton, N., 3 May 2019, In: Chemical Communications.

Research output: Contribution to journal › Article › peer-review

Open Access

Structural basis for enzymatic photocatalysis in chlorophyll biosynthesis

Zhang, S., Heyes, D. J., Feng, L., Sun, W., Johannissen, L. O., Liu, H., Levy, C. W., Li, X., Yang, J., Yu, X., Lin, M., Hardman, S. J. O., Hoeven, R., Sakuma, M., Hay, S., Leys, D., Rao, Z., Zhou, A., Cheng, Q. & Scrutton, N. S., 23 Oct 2019, In: Nature. 574, p. 722-725

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The Photochemical Spin Dynamics of the Vitamin B12 Derivative, Methylcobalamin

Lukinović, V., Woodward, J. R., Marrafa, T. C., Shanmugam, M., Heyes, D. J., Hardman, S. J. O., Scrutton, N. S., Hay, S., Fielding, A. J. & Jones, A. R., 2019, In: The Journal of Physical Chemistry B.

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Unexpected Roles of a Tether Harboring a Tyrosine Gatekeeper Residue in Modular Nitrite Reductase Catalysis

Hedison, T., Shenoy, R., Iorgu, A. I., Heyes, D., Fisher, K., Wright, G., Hay, S., Eady, R. R., Antonyuk, S., Hasnain, S. S. & Scrutton, N. S., 2019, In: ACS Catalysis.

Research output: Contribution to journal › Article › peer-review

2018

Photochemical mechanism of an atypical algal phytochrome

Choudry, U., Heyes, D., Hardman, S., Sakuma, M., Sazanovich, I. V., Woodhouse, J., De La Mora, E., Pedersen, M. N., Wulff, M., Weik, M., Schiro, G. & Scrutton, N., 18 May 2018, In: ChemBioChem: a European journal of chemical biology . 19, 10, p. 1036-1043 8 p.

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Pressurized CO₂as Carboxylating Agent for the Biocatalytic ortho-Carboxylation of Resorcinol

Plasch, K., Hofer, G., Keller, W., Hay, S., Heyes, D., Dennig, A., Glueck, S. M. & Faber, K., 2018, In: Green Chemistry.

Research output: Contribution to journal › Article › peer-review

Open Access

Stepwise Hydride Transfer in a Biological System: Insights into the Reaction Mechanism of the Light-Dependent Protochlorophyllide Oxidoreductase

Archipowa, N., Kutta, R., Heyes, D. & Scrutton, N., 1 Mar 2018, In: Angewandte Chemie. 57, 10, p. 2682-2686 4 p.

Research output: Contribution to journal › Article › peer-review

Open Access

2017

Direct evidence of an excited state triplet species upon photoactivation of the chlorophyll precursor protochlorophyllide

Brandariz De Pedro, G., Heyes, D., Hardman, S., Shanmugam, M., Jones, A., Weber, S., Nohr, D., Scrutton, N. & Fielding, A. (Corresponding), 2017, In: Journal of Physical Chemistry Letters.

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Open Access

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Excited State Properties of Protochlorophyllide Analogues and Implications for Light Driven Synthesis of Chlorophyll

Heyes, D., Hardman, S., Mansell, D., Ni Cheallaigh, A., Gardiner, J., Johannissen, L., Greetham, G. M., Towrie, M. & Scrutton, N., 2017, In: The Journal of Physical Chemistry Part B.

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2016

Cross-species analysis of protein dynamics associated with hydride and proton transfer in the catalytic cycle of the light-driven enzyme protochlorophyllide oxidoreductase.

Hoeven, R., Heyes, D., Hardman, S. & Scrutton, N., 2016, In: Biochemistry.

Research output: Contribution to journal › Article › peer-review

Open Access

Engineering Proximal vs Distal Heme-NO Coordination via Dinitrosyl Dynamics: Implications for NO Sensor Design

Kekilli, D., Petersen, C. A., Pixton, D. A., Ghafoor, D. D., Abdullah, G. H., Ghafoor, F. S. N., Wilson, M. T., Heyes, D., Hardman, S., Murphy, L. M., Strange, R. W., Scrutton, N., Andrew, C. R. & Hough, M. A., 2016, In: Chemical Science.

Research output: Contribution to journal › Article › peer-review

Open Access

Isolation and characterisation of 13 pterosins and pterosides from bracken (Pteridium aquilinum (L.) Kuhn) rhizome

Mohammad, R. H., Nur-E-Alam, M., Lahmann, M., Parveen, I., Tizzard, G. J., Coles, S. J., Fowler, M., Drake, A. F., Heyes, D. & Thoss, V., 1 Aug 2016, In: Phytochemistry. 128, p. 82-94 13 p.

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Open Access

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