Nancy Papalopulu

Prof

If you made any changes in Pure these will be visible here soon.

Personal profile

Biography

2006-present, Wellcome Trust Senior Research Fellow
Professor of Developmental Neuroscience, Faculty of Life Sciences, Manchester, UK

2000-2006, Wellcome Trust Senior Research Fellow
Wellcome Trust/Cancer Research UK Gurdon Institute, Cambridge, UK

1997-2000, Wellcome Trust Career Development Fellow
Wellcome Trust/Cancer Research UK Gurdon Institute, Cambridge, UK

1991-1996, Post-doctoral Fellow (HFSPO),
Laboratory of Molecular Neurobiology
The Salk Institute for Biomedical Research, La Jolla, USA.
Advisor: Prof. Chris Kintner

1986-1990, Ph.D., National Institute for Medical Research, London, UK.
Advisor: Prof. Robb Krumlauf

Research interests

During development, cells transition from a progenitor state to differentiation with defined timing, or to quiescence from which they may be reactivated. Understanding how such transitions and their timing are regulated is key in understanding how tissues are built, maintained, repaired or subverted in disease.

In recent years, our understanding of how cells make cell state transitions has been transformed by the application of single cell molecular technology that revealed a large degree of non-genetic heterogeneity in seemingly homogeneous populations of cells. In neural progenitors, single cell imaging with unstable reporters has revealed asynchronous pulsatile fluctuations in regulatory gene expression, which is masked by static measurements of population averages. Thus, cell fate transitions may not be driven simply by genes being turned on or off, but by a change in the dynamics of gene expression for example, from fluctuating or pulsatile expression to a more stable state.

Using single cell quantitative approaches, live imaging, multiple experimental model systems and mathematical modeling we have put forward the hypothesis that a mutually antagonistic interaction of a key neural progenitor transcription factor (TF) Hes1 and a microRNA, miR-9 is sufficient to generate pulsatile gene expression at the single cell level. This basic TF/miR regulatory network is capable of transitioning from pulsatile gene expression to a stable state autonomously and in a time-controlled manner. However, the timing can be “tuned” by external influences such as a change in parameters or the initial conditions. Finally, stochastic simulation of this TF/miR regulatory network led us to suggest that noise is beneficial in increasing progenitor robustness and spreading the time to differentiation.  We have experience with working with multiple model systems, including Xenopus, zebrafish, Embryonic Stem cell and Neural Stem cell culture,  quantitative approaches and molecular methods. We are particularly keen in conceptual integration of information coming from different fields.

Lab members

Dr. Cerys Manning, post-doc (sir Henry Wellcome Fellow)

Dr. Elli Marinopoulou, post-doc (sir Henry Wellcome Fellow)

Dr. Tom Pettini, post-doc

Dr. Ximena Soto, post-doc

Dr. Veronica Biga, post-doc

Nick Phillips, PhD student

Robert Lea-Research Technician

Peter Stanley-Research Assistant

Recent Previous members

Nitin Sabherwal-postdoc
Raphael Thuret-postdoc
Eamon Dubaissi-post-doc

Sarah Woolner-postdoc
Federico Dajas-Baillador-postdoc

Expertise related to UN Sustainable Development Goals

In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all. This person’s work contributes towards the following SDG(s):

  • SDG 3 - Good Health and Well-being

Fingerprint

Dive into the research topics where Nancy Papalopulu is active. These topic labels come from the works of this person. Together they form a unique fingerprint.
  • 1 Similar Profiles

Network

Recent external collaboration on country/territory level. Dive into details by clicking on the dots or