Sabine Flitsch

Search results

• 2017

  IRMPD spectroscopy sheds new (InfraRed) light on the sulfate pattern of carbohydrates

  Schindler, B., Barnes, L., Gray, J. C., Chambert, S., Flitsch, S., Oomens, J., Daniel, R., Allouche, A. R. & Compagnon, I., 2017, In: Journal of Physical Chemistry A. 121

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Structure 100%
  • NIR Spectroscopy 100%
  • Glycosaminoglycan 100%
  • Mid-IR Spectroscopy 50%
  • Vibrational Spectrum 50%
  328 Downloads (Pure)

• Label-Free Discovery Array Platform for the Characterization of Gly-can Binding Proteins and Glycoproteins

  Gray, C., Sanchez-Ruiz, A., Sardzikova, I., Ahmed, Y., Miller, R. L., Reyes Martinez, J., Pallister, E., Huang, K., Both, P., Hartmann, M., Roberts, H., Sardzik, R., Mandal, S., Turnbull, J., Eyers, C. & Flitsch, S., 18 Apr 2017, In: Analytical Chemistry. 89, 8, p. 4444-4451 8 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Glycoprotein 100%
  • Binding Protein 100%
  • Protein-Carbohydrate Interactions 100%
  • Protein Catabolism 50%
  • Glycosaminoglycan 50%
  283 Downloads (Pure)

• Real-time screening of biocatalysts in live bacterial colonies

  Yan, C., Parmeggiani, F., Jones, E. A., Claude, E., Hussain, S., Turner, N., Flitsch, S. & Barran, P., 2017, In: Journal of the American Chemical Society. 139, 4, p. 1408-1411 4 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Electrospray Ionization 100%
  • Biocatalyst 100%
  • Desorption 100%
  • Ion Mobility Mass Spectrometry 100%
  • Monooxygenase 100%
  100 Downloads (Pure)

• The self‐sufficient P450 RhF expressed in a whole cell system selectively catalyses the 5‐hydroxylation of diclofenac

  Klenk, J. M., Nebel, B., Porter, J., Kulig, J. K., Hussain, S., Richter, S. M., Tavanti, M., Turner, N., Hayes, M. A., Hauer, B. & Flitsch, S., 2017, In: Biotechnology Journal (Print).

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • 5-Hydroxydiclofenac 100%
  611 Downloads (Pure)
• 2016

  Applications of ion mobility mass spectrometry for high throughput, high resolution glycan analysis

  Gray, C., Thomas, B., Upton, A., Migas, L., Eyers, C. E., Barran, P. & Flitsch, S., 2016, In: Biochimica et Biophysica Acta (BBA)-General Subjects.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Polysaccharides 100%
  • Mass Spectrometry 100%
  • Ion Mobility Mass Spectrometry 100%
  • Structure 22%
  • Ion-Mobility Spectrometry 20%
  1242 Downloads (Pure)

• Enantioselective Benzylic Hydroxylation Catalysed by P450 Monooxygenases: Characterisation of a P450cam Mutant Library and Molecular Modelling

  Eichler, A., Gricman, Ł., Herter, S., Kelly, P., Turner, N., Pleiss, J. & Flitsch, S., 2 Mar 2016, In: CHEMBIOCHEM. 17, 5, p. 426-432 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Monooxygenase 100%
  • Hydroxylation 100%
  • Enantioselectivity 100%
  • Molecular Modeling 100%
  • Unspecific Monooxygenase 100%

• Fabrication and Application of Isotopically-Labelled Gold Arrays for Multiplexed Peptide Analysis

  Castangia, R., Hudson, S. R., Robinson, H. K., Flitsch, S., Thomas-Oates, J. & Routledge, A., 2016, In: CHEMBIOCHEM. 17, 21

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File
  37 Downloads (Pure)

• Inexpensive and fast pathogenic bacteria screening using field-effect transistors

  Formisano, N., Bhalla, N., Heeran, M., Reyes Martinez, J., Sarkar, A., Laabei, M., Jolly, P., Bowen, C. R., Taylor, J. T., Flitsch, S. & Estrela, P., 15 Nov 2016, In: Biosensors and Bioelectronics. 85, p. 103-109 7 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File
  351 Downloads (Pure)

• One Pot Cascade Synthesis of Mono- and Di-Substituted Piperidines and Pyrrolidines using Carboxylic Acid Reductase (CAR), ω-Transaminase (ω-TA) and Imine Reductase (IRED) Biocatalysts

  France, S., Hussain, S., Hill, A., Hepworth, L., Howard, R. M., Mulholland, K. R., Flitsch, S. & Turner, N., 2 May 2016, In: ACS Catalysis. 6, 6, p. 3753-3759

  Research output: Contribution to journal › Article › peer-review

  • Imine 100%
  • Biocatalyst 100%
  • Piperidine 100%
  • Carboxylic Acid 100%
  • Pyrrolidine 100%

• Rapid and sensitive monitoring of biocatalytic reactions using ion mobility mass spectrometry

  Yan, C., Schmidberger, J., Parmeggiani, F., Hussain, S., Turner, N., Flitsch, S. & Barran, P., 2016, In: Analyst.

  Research output: Contribution to journal › Article › peer-review

  • Ion Mobility Mass Spectrometry 100%
  • Unspecific Monooxygenase 50%
  • Isotope Labeling 50%
  • Triacylglycerol Lipase 50%
  • Pancreatic Lipase 50%

• Synthesis of Enantiomerically Pure Ring-Substituted l-Pyridylalanines by Biocatalytic Hydroamination

  Ahmed, S., Parmeggiani, F., Weise, N., Flitsch, S. & Turner, N., 2016, In: Organic Letters.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Biocatalyst 100%
  • Purity 100%
  • Enantiopurity 100%
  • Hydroamination 100%
  • Phenylalanine 100%
  67 Downloads (Pure)

• Whole-cell biocatalysts for stereoselective C-H amination reactions

  Both, P., Kelly, P., Mutti, F., Turner, N. & Flitsch, S., 18 Jan 2016, In: Angewandte Chemie (International Edition).

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Biocatalyst 100%
  • Donor 50%
  • Purity 50%
  • Cofactor 50%

• Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s

  Weissenborn, M. J., Notonier, S., Lang, S. L., Otte, K. B., Herter, S., Turner, N., Flitsch, S. & Hauer, B., 2016, In: Chemical Communications. 52, 36, p. 6158-6161 4 p.

  Research output: Contribution to journal › Article › peer-review

  • Cell Screening 100%
  • Marinobacter Hydrocarbonoclasticus 100%
• 2015

  Active site diversification of P450cam with indole generates catalysts for benzylic oxidation reactions

  Flitsch, S., Kelly, P., Eichler, A., Herter, S., Kranz, D. C. & Turner, N., Sept 2015, In: Beilstein Journal of Organic Chemistry. 11, p. 1713-1720

  Research output: Contribution to journal › Article › peer-review

• A recycling pathway for cyanogenic glycosides evidenced by the comparative metabolic profiling in three cyanogenic plant species

  Flitsch, S., Picmanova, M., Neilson, E. H., Motawia, M. S., Olsen, C. E., Agerbirk, N., Gray, C., Meier, S., Silvestro, D., Jorgensen, K., Sanchez-Perez, R., Moller, B. L. & Bjarnholt, N., 1 Aug 2015, In: The Biochemical Journal. 469, 3, p. 375-389

  Research output: Contribution to journal › Article › peer-review

• Biochemical characterisation of the neuraminidase pool of the human gut symbiont Akkermansia muciniphila

  Flitsch, S., Huang, K., Wang, M. M., Kulinich, A., Yao, H. L., Ma, H. Y., Reyes Martinez, J., Duan, X. C., Chen, H., Cai, Z. P. & Voglmeir, J., 13 Oct 2015, In: Carbohydrate Research. 415, p. 60-65

  Research output: Contribution to journal › Article › peer-review

• Biological and biochemical properties of two Xenopus laevis N-acetylgalactosaminyltransferases with contrasting roles in embryogenesis

  Voglmeir, J., Laurent, N., Flitsch, S. L., Oelgeschlaeger, M. & Wilson, I. B. H., Feb 2015, In: Comparative Biochemistry and Physiology. Part B: Biochemistry & Molecular Biology . 180, p. 40-47 7 p.

  Research output: Contribution to journal › Article › peer-review

• Chemoenzymatic Synthesis of Optically Pure L- and D-Biarylalanines through Biocatalytic Asymmetric Amination and Palladium-Catalyzed Arylation

  Ahmed, S., Parmeggiani, F., Weise, N., Flitsch, S. & Turner, N., Sept 2015, In: ACS Catalysis. 5, p. 5410-5413

  Research output: Contribution to journal › Article › peer-review

• Copper-catalyzed double additions and radical cyclization cascades in the re-engineering of the antibacterial pleuromutilin

  Ruscoe, R. E., Huang, H., Flitsch, S. L. & Procter, D. J., 3 Nov 2015, In: Chemistry: A European Journal.

  Research output: Contribution to journal › Article › peer-review

• Enzymatic synthesis of Amides

  Flitsch, S. L., Schmidberger, J. W., Hepworth, L. J. & Green, A. P., 2015, Science of Synthesis, Biocatalysis in Organic Synthesis. Georg Thieme Verlag, p. 329-373

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

• Functionalized graphene

  Blanford, C. F. (Inventor), Wehner, M. (Inventor) & Flitsch, S. (Inventor), 12 Nov 2015, Patent No. US10173898B2, 11 May 2015, Priority date 9 May 2014, Priority No. GB1408221.8

  Research output: Patent

  Open Access

  • Graphene 100%
  • Functional Group 33%
  • Chemical Synthesis 16%

• Glycosyltransferases

  Flitsch, S. L. & Voglmeir, J., 2015, Science of Synthesis, Biocatalysis in Organic Synthesis. 1 ed. Georg Thieme Verlag, p. 507-538

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

• Voices of chemical biology

  Flitsch, S., Bertozzi, C. R., Stockwell, B. R., Kubicek, S., Dickinson, B. C., Chang, C. J., Schultz, C., Silver, P. A., Gestwicki, J. E., Chiosis, G., Chattopadhyay, A., Butcher, R. A., Park, S. B., Shoichet, B. K., Zhang, J., Liu, D. R., Ohnishi, Y., Weerapana, E., Williams, A. H. & He, C. & 11 others, Moroni, A., Gerhard, T., Chang, Y.-T., Waldmann, H., Bogyo, M., Oddershede, L. B., Christopoulos, A., Imperiali, B., Ehrlich, H., Xing, C. & Prescher, J. A., Jun 2015, In: Nature chemical biology. 11, p. 378-379 2 p.

  Research output: Contribution to journal › Article › peer-review
• 2014

  2-Pyridylfuran: A New Fluorescent Tag for the Analysis of Carbohydrates

  Cai, Z. P., Hagan, A. K., Wang, M. M., Flitsch, S. L., Liu, L. & Voglmeir, J., 27 Apr 2014, In: Analytical Chemistry. 86, 10, p. 5179-5186 7 p.

  Research output: Contribution to journal › Article › peer-review

• Discrimination of epimeric glycans and glycopeptides using IM-MS and its potential for carbohydrate sequencing

  Both, P., Green, A. P., Gray, C. J., Sardzík, R., Voglmeir, J., Fontana, C., Austeri, M., Rejzek, M., Richardson, D., Field, R. A., Widmalm, G., Flitsch, S. L. & Eyers, C. E., Jan 2014, In: Nature Chemistry. 6, 1, p. 65-74 10 p.

  Research output: Contribution to journal › Article › peer-review

  • Uridine Diphosphate N-Acetylglucosamine 100%
  • N-Acetylgalactosamine 75%

• Glycosylation Characterization of Human and Porcine Fibrinogen Proteins by Lectin-Binding Biophotonic Microarray Imaging

  ReyeOlkhov, R. V., Weissenborn, M. J., Flitsch, S. L. & Shaw, A. M., 7 Jan 2014, In: Analytical Chemistry. 86, 1, p. 621-628 7 p.

  Research output: Contribution to journal › Article › peer-review

• Sialylation of lactosyl lipids in membrane microdomains by T.cruzi trans-sialidase

  Noble, G., Craven, F., Segarra-Maset, M., Reyes Martinez, J., Sardzik, R., Flitsch, S. L. & Webb, S., 7 Dec 2014, In: Organic & biomolecular chemistry. 12, 45, p. 9272-9278 6 p.

  Research output: Contribution to journal › Article › peer-review

  • Glycolipid 100%
  • Sialylation 100%
  • Sialidase 100%
  • Neuraminidase 100%
  • Membrane Microdomain 100%
• 2013

  Deubiquitinases regulate the activity of caspase-1 and interleukin-1β secretion via assembly of the inflammasome

  Lopez-Castejon, G., Luheshi, N. M., Compan, V., High, S., Whitehead, R. C., Flitsch, S., Kirov, A., Prudovsky, I., Swanton, E. & Brough, D., 25 Jan 2013, In: Journal of Biological Chemistry. 288, 4, p. 2721-2733 13 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • Interleukin-1 100%
  • Secretion (Process) 100%
  • Caspase 1 100%
  • Inflammasome 100%
  • Ubiquitin 75%

• Discovery of novel human aquaporin-1 blockers

  Seeliger, D., Zapater, C., Krenc, D., Haddoub, R., Flitsch, S., Beitz, E., Cerdà, J. & De Groot, B. L., 18 Jan 2013, In: ACS chemical biology. 8, 1, p. 249-256 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Aquaporin 100%
  • Aquaporin 1 100%
  • Mutagenesis 14%
  • Oocyte 14%
  • Xenopus laevis 14%

• Enzymatic reactions on immobilised substrates

  Gray, C. J., Weissenborn, M. J., Eyers, C. E. & Flitsch, S. L., 2013, In: Chemical Society Reviews. 42, 15, p. 6378-6405 28 p.

  Research output: Contribution to journal › Article › peer-review

• Enzymatic synthesis of colorimetric substrates to determine α-2,3- and α-2,6-specific neuraminidase activity

  Reyes Martínez, J. E., Šardzík, R., Voglmeir, J. & Flitsch, S. L., 2013, In: RSC Advances. 3, 44, p. 21335-21338 3 p.

  Research output: Contribution to journal › Article › peer-review

  • Sialidase 100%
  • Neuraminidase 100%
  • Surface Property 50%
  • Lectin 50%
  • Gene Linkage 50%

• Profiling Glycosyltransferase Activities by Tritium Imaging of Glycan Microarrays

  Serna, S., Hokke, C. H., Weissenborn, M., Flitsch, S., Martin-Lomas, M. & Reichardt, N. C., 10 May 2013, In: ChemBioChem: a European journal of chemical biology . 14, 7, p. 862-869 7 p.

  Research output: Contribution to journal › Article › peer-review

• Substrate promiscuity of cytochrome P450 RhF

  O'Reilly, E., Corbett, M., Hussain, S., Kelly, P. P., Richardson, D., Flitsch, S. L. & Turner, N. J., Jun 2013, In: Catalysis Science and Technology. 3, 6, p. 1490-1492 2 p.

  Research output: Contribution to journal › Article › peer-review

  • Cytochrome P450 100%
  • Alpha Oxidation 50%
  • Epoxidation 50%
  • Hydroxylation 50%
  • DNA Template 50%
• 2012

  Accelerated enzymatic galactosylation of N -acetylglucosaminolipids in lipid microdomains

  Noble, G. T., Craven, F. L., Voglmeir, J., Šardzík, R., Flitsch, S. L. & Webb, S. J., 8 Aug 2012, In: Journal of the American Chemical Society. 134, 31, p. 13010-13017 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Glycolipid 100%
  • Lipid Microdomain 100%
  • Liquid Chromatography Mass Spectrometry 100%
  • Glycocalyx 100%
  • Liquid Chromatography-Mass Spectrometry 66%

• Chemoenzymatic synthesis of O-mannosylpeptides in solution and on solid phase

  Šardzík, R., Green, A., Laurent, N., Both, P., Fontana, C., Voglmeir, J., Weissenborn, M. J., Haddoub, R., Grassi, P., Haslam, S. M., Widmalm, G. & Flitsch, S. L., 14 Mar 2012, In: Journal of the American Chemical Society. 134, 10, p. 4521-4524 3 p.

  Research output: Contribution to journal › Article › peer-review

  • Solution and Solubility 100%
  • Solid 100%
  • Glycopeptide 100%
  • Tetrasaccharide 66%
  • Glycosylation 33%

• Dual purpose S-trityl-linkers for glycoarray fabrication on both polystyrene and gold

  Wehner, J. W., Weissenborn, M. J., Hartmann, M., Gray, C. J., Sardzik, R., Eyers, C. E., Flitsch, S. L., Lindhorst, T. K. & Šardzík, R., 2012, In: Organic & biomolecular chemistry. 10, 44, p. 8919-8926 8 p.

  Research output: Contribution to journal › Article › peer-review

• Enzymatic amine acyl exchange in peptides on gold surfaces

  Castangia, R., Austeri, M. & Flitsch, S. L., 21 Dec 2012, In: Angewandte Chemie - International Edition. 51, 52, p. 13016-13018 2 p.

  Research output: Contribution to journal › Article › peer-review

  • Carboxamide 100%
  • Carboxylate 83%
  • Carboxylic Acid 60%
  • Chemoselectivity 40%
  • Biocompatible Material 20%

• Formation of carbohydrate-functionalised polystyrene and glass slides and their analysis by MALDI-TOF MS

  Weissenborn, M. J., Wehner, J. W., Gray, C. J., Šardzík, R., Eyers, C. E., Lindhorst, T. K., Flitsch, S. L. & Sardzik, R., 21 May 2012, In: Beilstein Journal of Organic Chemistry. 8, p. 753-762 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Matrix-Assisted Laser Desorption-Ionization 100%
  • Time-of-Flight Mass Spectrometry 100%
  • Polystyrene 100%
  • Mass Spectrometry 100%
  • Matrix Assisted Laser Desorption Ionization - Time of Flight (MALDI-TOF) 100%

• Glycoarrays on gold surfaces

  Both, P., Sardzik, R., Weissenborn, M. J., Green, A. P., Voglmeir, J. & Flitsch, S. L., 2012, host publication.

  Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

• Glycoarrays on Gold Surfaces

  Both, P., Sardzik, R., Weissenborn, M., Green, A., Voglmeir, J. & Flitsch, S., 11 Jul 2012, Cracking the Sugar Code by Navigating the Glycospace. p. 93-105

  Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

• Inhibition of protein translocation at the endoplasmic reticulum promotes activation of the unfolded protein response

  McKibbin, C., Mares, A., Piacenti, M., Williams, H., Roboti, P., Puumalainen, M., Callan, A. C., Lesiak-Mieczkowska, K., Linder, S., Harant, H., High, S., Flitsch, S. L., Whitehead, R. C. & Swanton, E., 15 Mar 2012, In: Biochemical Journal. 442, 3, p. 639-648 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Electrospray Ionization 100%
  • Endoplasmic Reticulum Associated Degradation 100%

• MALDI-ToF MS analysis of glycosyltransferase activities on gold surface arrays

  Laurent, N., Haddoub, R., Voglmeir, J. & Flitsch, S. L., 2012, In: Methods in Molecular Biology. 808, p. 269-284 15 p.

  Research output: Contribution to journal › Article › peer-review

  • Surface Property 100%
  • Matrix-Assisted Laser Desorption-Ionization 100%
  • Glycosyltransferase 100%
  • Glycoside Hydrolases 33%
  • Interaction Study 33%

• Oxo-ester mediated native chemical ligation on microarrays: An efficient and chemoselective coupling methodology

  Weissenborn, M. J., Castangia, R., Wehner, J. W., Šardzík, R., Lindhorst, T. K. & Flitsch, S. L., 11 May 2012, In: Chemical Communications. 48, 37, p. 4444-4446 2 p.

  Research output: Contribution to journal › Article › peer-review

  • Microarrays 100%
  • Chemoselectivity 100%
  • Glycoconjugates 50%
  • Carboxamide 50%
  • Thiol 50%

• Regio- and stereoselective oxidation of unactivated C-H bonds with Rhodococcus rhodochrous

  O'Reilly, E., Aitken, S. J., Grogan, G., Kelly, P. P., Turner, N. J. & Flitsch, S. L., 3 Apr 2012, In: Beilstein Journal of Organic Chemistry. 8, p. 496-500 4 p.

  Research output: Contribution to journal › Article › peer-review

  • Rhodococcus rhodochrous 100%

• Regio- and stereo-selective oxidation of unactivated C-H bonds with Rhodococcus rhodochrous

  O'Reilly, E., Aitken, S. J., Grogan, G., Kelly, P. P., Turner, N. J. & Flitsch, S. L., 2012, In: Beilstein Journal of Organic Chemistry. 8, p. 496-500

  Research output: Contribution to journal › Article › peer-review

• Synthesis and biological evaluation of novel inhibitors of protein biogenesis at the endoplasmic reticulum

  Whitehead, R., Flitsch, S., High, S. & Swanton, L., 2012, In: Unknown Journal. 223 p.

  Research output: Contribution to journal › Article › peer-review

  • Endoplasmic-Reticulum-Associated Protein Degradation 100%
  • Click 100%
  • Secretion (Process) 100%
  • Biogenesis 100%
  • Deubiquitination 100%
• 2011

  Biochemical correlation of activity of the α-dystroglycan-modifying glycosyltransferase POMGnT1 with mutations in muscle-eye-brain disease

  Voglmeir, J., Kaloo, S., Laurent, N., Meloni, M. M., Bohlmann, L., Wilson, I. B. H. & Flitsch, S. L., 1 Jun 2011, In: Biochemical Journal. 436, 2, p. 447-455 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Dystroglycan 100%

• Biocompatible functionalisation of starch

  Alissandratos, A., Baudendistel, N., Hauer, B., Baldenius, K., Flitsch, S. & Halling, P., 14 Jan 2011, In: Chemical Communications. 47, 2, p. 683-685 2 p.

  Research output: Contribution to journal › Article › peer-review

  • Biocompatible Material 100%
  • Azide 100%
  • Fatty Acids 100%
  • Alkyne 50%
  • Esterification 50%

• Chemoenzymatic synthesis of sialooligosaccharides on arrays for studies of cell surface adhesion

  Šardzík, R., Sharma, R., Kaloo, S., Voglmeir, J., Crocker, P. R. & Flitsch, S. L., 21 May 2011, In: Chemical Communications. 47, 19, p. 5425-5427 2 p.

  Research output: Contribution to journal › Article › peer-review

  • Surface Property 100%
  • Glycosylation 50%
  • Cell Adhesion 50%

• Chimeric self-sufficient P450cam-RhFRed biocatalysts with broad substrate scope

  Robin, A., Köhler, V., Jones, A., Ali, A., Kelly, P. P., O'Reilly, E., Turner, N. J. & Flitsch, S. L., 2 Nov 2011, In: Beilstein Journal of Organic Chemistry. 7, p. 1494-1498 4 p., 7.

  Research output: Contribution to journal › Article › peer-review

  • Biocatalyst 100%
  • High Throughput Screening 50%