Tim Hardingham, BSc, PhD, DSc

Search results

• 2020

  Generation of Human Induced Pluripotent Stem Cells from Anterior Cruciate Ligament

  Woods, S., Bates, N., Dunn, S. L., Serracino‐Inglott, F., Hardingham, T. E. & Kimber, S. J., 25 Jan 2020, In: Journal of Orthopaedic Research. 38, 1

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • Induced Pluripotent Stem Cell 100%
  • Reprogramming 100%
  • Anterior Cruciate Ligament 100%
  • Dermal Fibroblast 42%
• 2019

  Enhanced chondrogenesis from human embryonic stem cells

  Wang, T., Nimkingratana, P., Smith, C. A., Cheng, A., Hardingham, T. & Kimber, S., 9 Jul 2019, (E-pub ahead of print) In: Stem Cell Research.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • Human Embryonic Stem Cell 100%
  • Chondrogenesis 100%
  • Bone Morphogenetic Protein 2 100%
  • Bone Morphogenetic Protein 4 83%
  • Gene Expression 33%

• Identification of long non-coding RNAs expressed in knee and hip osteoarthritic cartilage

  Ajekigbe, B., Cheung, K., Xu, Y., Skelton, A. J., Panagiotopoulos, A., Soul, J., Hardingham, T. E., Deehan, D. J., Barter, M. J. & Young, D. A., 2019, In: Osteoarthritis and Cartilage.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • Untranslated RNA 100%
  • Long Untranslated RNA 100%
  • Knee 100%
  • Hip 100%
  • Non-Coding RNA 100%

• SkeletalVis: an exploration and meta-analysis data portal of cross-species skeletal transcriptomics data

  Soul, J., Hardingham, T., Boot-Handford, R. & Schwartz, J.-M., Jul 2019, In: Bioinformatics. 35, p. 2283-2290

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • Meta-Analysis 100%
  • Transcriptomics 100%
  • Available Expression 100%
  • Bone Disease 66%
  • Gene Expression 33%
• 2018

  Stratification of knee osteoarthritis: two major patient subgroups identified by genome wide expression analysis of articular cartilage.

  Soul, J., Dunn, S., Anand, S., Serracino-Inglott, F., Schwartz, J.-M., Boot-Handford, R. & Hardingham, T., Mar 2018, In: Annals of the rheumatic diseases. 77, p. 423-430

  Research output: Contribution to journal › Article › peer-review

  Open Access
• 2016

  Gene expression changes in damaged osteoarthritic cartilage identify a signature of non-chondrogenic and mechanical responses

  Dunn, S., Soul, J., Anand, S., Schwartz, J.-M., Boot-Handford, R. & Hardingham, T., Aug 2016, In: Osteoarthritis and Cartilage. 24, 8, p. 1431-1440 10 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • Gene Expression 100%
  • System Analysis 100%
  • Osteoarthritis 100%
  • RNA Sequencing 66%
  • Proteases 66%

• PhenomeScape: a Cytoscape app to identify differentially regulated sub-networks using known disease associations

  Soul, J., Dunn, S., Hardingham, T., Boot-Handford, R. & Schwartz, J.-M., 15 Dec 2016, In: Bioinformatics (Oxford, England). 32, 24, p. 3847-9

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • Protein Interaction 100%
  • Gene Expression 100%
  • Bioinformatics Software 100%
  • Gene Expression Data 50%
• 2015

  PhenomeExpress: A refined network analysis of expression datasets by inclusion of known disease phenotypes

  Soul, J., Hardingham, T., Boot-Handford, R. & Schwartz, J.-M., 29 Jan 2015, In: Scientific Reports. 5, 8117.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • Protein-Protein Interaction 100%
  • Mouse 50%
  • Transcriptomics 50%
• 2014

  Assembly of the respiratory Mucin MUC5B a new model for a gel-forming Mucin

  Ridley, C., Kouvatsos, N., Raynal, B. D., Howard, M., Collins, R. F., Desseyn, J. L., Jowitt, T. A., Baldock, C., Davis, C. W., Hardingham, T. E. & Thornton, D. J., 6 Jun 2014, In: Journal of Biological Chemistry. 289, 23, p. 16409-16420 11 p.

  Research output: Contribution to journal › Article › peer-review

  • MUC5B 100%
  • Mucin 100%
  • Polymerization 30%
  • Disulfide 30%
  • Cross-Link 20%

• Cartilage Repair Using Human Embryonic Stem Cell-Derived Chondroprogenitors.

  Cheng, A., Kapacee, Z., Peng, J., Lu, S., Lucas, R. J., Hardingham, T. E. & Kimber, S. J., 1 Oct 2014, In: Stem Cells Translational Medicine. 3, 11, p. 1287-1294

  Research output: Contribution to journal › Article › peer-review

  • Nude Rat 100%

• Generating Cartilage Repair from Pluripotent Stem Cells

  Cheng, A., Hardingham, T. & Kimber, S., 2014, In: Tissue Engineering. Part B. Reviews . 20, 4, p. 257-266 9 p.

  Research output: Contribution to journal › Article › peer-review
• 2010

  A chemically-defined protocol for generating chondrocytes from human embryonic stem cells.

  Oldershaw, R., Baxter, M., Lowe, E., Bates, N., Grady, L., Soncin, F., Brison, D., Hardingham, T. & Kimber, S., 2010, In: Nature biotechnology. 28, 11

  Research output: Contribution to journal › Article › peer-review

  • Embryonic Stem Cell 100%
  • Chondrocyte 100%
  • Directed Differentiation 33%
  • Cellular Differentiation 33%
  • Cell Lineage 33%

• Directed differentiation of human embryonic stem cells toward chondrocytes

  Oldershaw, R. A., Baxter, M. A., Lowe, E. T., Bates, N., Grady, L. M., Soncin, F., Brison, D. R., Hardingham, T. E. & Kimber, S. J., Nov 2010, In: Nature biotechnology. 28, 11, p. 1187-1194 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Directed Differentiation 100%
  • Embryonic Stem Cell 100%
  • Chondrocyte 100%
  • Cell Aggregation 66%
  • Glycosaminoglycan 33%

• Fell-Muir lecture: Cartilage 2010 - The known unknowns

  Hardingham, T. E., Jun 2010, In: International Journal of Experimental Pathology. 91, 3, p. 203-209 6 p.

  Research output: Contribution to journal › Article › peer-review

  • Matrix 100%
  • Molecular Property 100%

• Gene expression in human chondrocytes in late OA is changed in both fibrillated and intact cartilages without evidence of generalised chondrocyte hypertrophy

  Brew, C., Clegg, P., Boot-Handford, R., Andrews, T. & Hardingham, T., 2010, In: Annals Rheum Dis. 467

  Research output: Contribution to journal › Article › peer-review

• Gene expression in human chondrocytes in late osteoarthritis is changed in both fibrillated and intact cartilage without evidence of generalised chondrocyte hypertrophy

  Brew, C. J., Clegg, P. D., Boot-Handford, R. P., Andrew, J. G. & Hardingham, T., Jan 2010, In: Annals of the rheumatic diseases. 69, 1, p. 234-240 6 p.

  Research output: Contribution to journal › Article › peer-review

  • Gene Expression 100%
  • Osteoarthritis 100%
  • Chondrocyte 100%
  • Aggrecan 20%
  • Regulator Gene 20%

• Hardingham T (2010) Cell and Tissue-based Approaches for Cartilage Repair

  Hardingham, T., 2010, In: ALTERNATIVES TO LABORATORY ANIMALS. 38, p. 35-39

  Research output: Contribution to journal › Article › peer-review

• Notch signaling during chondrogenesis of human bone marrow stem cells

  Oldershaw, R. A. & Hardingham, T. E., Feb 2010, In: Bone. 46, 2, p. 286-293 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Chondrogenesis 100%
  • Notch Signaling 100%
  • Hematopoietic Stem Cell 100%
  • Notch Signaling Pathway 100%
  • Notch 71%

• Order within disorder: Aggrecan chondroitin sulphate-attachment region provides new structural insights into protein sequences classified as disordered

  Jowitt, T. A., Murdoch, A. D., Baldock, C., Berry, R., Day, J. M. & Hardingham, T. E., Dec 2010, In: Proteins: Structure, Function and Bioinformatics. 78, 16, p. 3317-3327 10 p.

  Research output: Contribution to journal › Article › peer-review

  • Peptide Sequence 100%
  • Chondroitin Sulfate 100%
  • Aggrecan 100%
  • Protein Secondary Structure 50%
  • X Ray 16%

• The potential of stem cells in the treatment of knee cartilage defects

  Khan, W. S., Johnson, D. S. & Hardingham, T. E., Dec 2010, In: The Knee. 17, 6, p. 369-374 5 p.

  Research output: Contribution to journal › Article › peer-review

  • Defects 100%
  • Stem Cell 100%
  • Mesenchymal Stem Cell 50%
  • Cell Source 50%
  • Hyalin 40%
• 2009

  Hyperosmolarity regulates SOX9 mRNA posttranscriptionally in human articular chondrocytes

  Tew, S. R., Peffers, M. J., McKay, T. R., Lowe, E. T., Khan, W. S., Hardingham, T. E. & Clegg, P. D., 2009, In: American Journal of Physiology: Cell Physiology. 297, 4, p. C898-C906

  Research output: Contribution to journal › Article › peer-review

  • Hyperosmolarity 100%
  • 4 (4 Fluorophenyl) 2 (4 Hydroxyphenyl) 5 (4 Pyridyl)imidazole 33%
  • Cycloheximide 33%
  • mRNA Expression Level 12%

• Notch signaling during chondrogenesis of bone marrow stem cells

  Hardingham, T. & Oldershaw, R., 2009, In: Bone. 350

  Research output: Contribution to journal › Article › peer-review

• The epitope characterisation and the osteogenic differentiation potential of human fat pad-derived stem cells is maintained with ageing in later life

  Khan, W. S., Adesida, A. B., Tew, S. R., Andrew, J. G. & Hardingham, T. E., Feb 2009, In: Injury. 40, 2, p. 150-157 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Stem Cell 100%
  • Fat Pad 100%
  • Bone Development 40%
  • Cell Culture 40%
  • Alkaline Phosphatase 40%

• The influence of donor and hypoxic conditions on the assembly of cartilage matrix by osteoarthritic human articular chondrocytes on Hyalograft matrices

  Katopodi, T., Tew, S. R., Clegg, P. D. & Hardingham, T. E., Feb 2009, In: Biomaterials. 30, 4, p. 535-540 5 p.

  Research output: Contribution to journal › Article › peer-review

  • Articular Cartilage Repair 100%
• 2008

  Cellular methods in cartilage research: Primary human chondrocytes in culture and chondrogenesis in human bone marrow stem cells

  Tew, S. R., Murdoch, A. D., Rauchenberg, R. P. & Hardingham, T. E., May 2008, In: Methods. 45, 1, p. 2-9 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Chondrogenesis 100%
  • Chondrocyte 100%
  • Hematopoietic Stem Cell 100%
  • Cartilage 100%
  • In Vitro 50%

• Extracellular matrix and pathogenic mechanisms in osteoarthritis

  Hardingham, T., Jan 2008, In: Current Rheumatology Reports. 10, 1, p. 30-36 6 p.

  Research output: Contribution to journal › Article › peer-review

  • Aggrecan 100%
  • Metalloproteinase 100%
  • Metalloendopeptidase 100%
  • Thrombospondin 100%
  • Disintegrin 100%

• Human infrapatellar fat pad-derived stem cells express the pericyte marker 3G5 and show enhanced chondrogenesis after expansion in fibroblast growth factor-2

  Khan, W. S., Tew, S. R., Adesida, A. B. & Hardingham, T. E., 3 Jul 2008, In: Arthritis Research and Therapy. 10, 4, R74.

  Research output: Contribution to journal › Article › peer-review

  • Chondrogenesis 100%
  • Basic Fibroblast Growth Factor 100%
  • Cell Aggregation 33%
  • Alanine Aminopeptidase 16%
  • CD90 16%

• Hyaluronan binding to Link module of TSG-6 and to G1 domain of aggrecan is differently regulated by pH

  Boon, C. H., Gribbon, P. M., Day, A. J. & Hardingham, T. E., 21 Nov 2008, In: Journal of Biological Chemistry. 283, 47, p. 32294-32301 7 p.

  Research output: Contribution to journal › Article › peer-review

  • TSG-6 100%
  • Hyaluronan 100%
  • Restylane 100%
  • Aggrecan 100%
  • Binding Affinity 50%

• Notch signaling through Jagged-1 is necessary to initiate chondrogenesis in human bone marrow stromal cells but must be switched off to complete chondrogenesis

  Oldershaw, R. A., Tew, S. R., Russell, A. M., Meade, K., Hawkins, R., McKay, T. R., Brennan, K. R. & Hardingham, T. E., Mar 2008, In: Stem Cells. 26, 3, p. 666-674 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Phenylglycine 100%

• SOX9 transduction increases chondroitin sulfate synthesis in cultured human articular chondrocytes without altering glycosyltransferase and sulfotransferase transcription

  Tew, S. R., Pothacharoen, P., Katopodi, T. & Hardingham, T. E., 1 Sept 2008, In: Biochemical Journal. 414, 2, p. 231-236 5 p.

  Research output: Contribution to journal › Article › peer-review

  • Transcription 100%
  • Chondroitin Sulfate 100%
  • Chondrocyte 100%
  • Glycosyltransferase 100%
  • SOX9 100%
• 2007

  Chondrogenic differentiation of human bone marrow stem cells in transwell cultures: Generation of scaffold-free cartilage

  Murdoch, A. D., Grady, L. M., Ablett, M. P., Katopodi, T., Meadows, R. S. & Hardingham, T. E., Nov 2007, In: Stem Cells. 25, 11, p. 2786-2796 10 p.

  Research output: Contribution to journal › Article › peer-review

  • Proteoglycan 100%
  • Clinical Study 100%
  • Transcription Factors 100%
  • Aggrecan 100%
  • Collagen Type 2 100%

• Differential cartilaginous tissue formation by human synovial membrane, fat pad, meniscus cells and articular chondrocytes

  Marsano, A., Millward-Sadler, S. J., Salter, D. M., Adesida, A., Hardingham, T., Tognana, E., Kon, E., Chiari-Grisar, C., Nehrer, S., Jakob, M. & Martin, I., Jan 2007, In: Osteoarthritis and Cartilage. 15, 1, p. 48-58 10 p.

  Research output: Contribution to journal › Article › peer-review

  • Cartilage 100%
  • Synovial Membrane 100%
  • Fat Pad 100%
  • Chondrocyte 100%
  • Glycosaminoglycan 66%

• Human meniscus cells express hypoxia inducible factor-1alpha and increased. SOX9 in response to low oxygen tension in cell aggregate culture

  Adesida, A., Grady, L., Khan, W., Millward-Sadler, S., Salter, D. & Hardingham, T., 2007, In: Arthritis Research and Therapy. 9, 4

  Research output: Contribution to journal › Article › peer-review

• Hypoxic conditions increase hypoxia-inducible transcription factor 2alpha and enhance chondrogenesis in stem cells from the infrapatellar fat pad of osteoarthritis patients.

  Khan, W., Adesida, A. & Hardingham, T., 2007, In: Arthritis Research and Therapy. 9, 3

  Research output: Contribution to journal › Article › peer-review

• SOX9 transduction of a human chondrocytic cell line identifies novel genes regulated in primary human chondrocytes and in osteoarthritis

  Tew, S. R., Clegg, P. D., Brew, C. J., Redmond, C. M. & Hardingham, T. E., 12 Oct 2007, In: Arthritis Research and Therapy. 9, 5, R107.

  Research output: Contribution to journal › Article › peer-review

  • Chondrocyte 100%
  • SOX9 100%
  • Primary Culture 10%
  • Gene Expression 10%
  • Microarrays 10%

• Two related but distinct chondroitin sulfate mimetope octasaccharide sequences recognized by monoclonal antibody WF6

  Pothacharoen, P., Kalayanamitra, K., Deepa, S. S., Fukui, S., Hattori, T., Fukushima, N., Hardingham, T., Kongtawelert, P. & Sugahara, K., 30 Nov 2007, In: Journal of Biological Chemistry. 282, 48, p. 35232-35246 14 p.

  Research output: Contribution to journal › Article › peer-review

  • Primary Structure 100%
  • Octasaccharide 100%
  • Chondroitin Sulfate 100%
  • Cartilage 100%
  • Monoclonal Antibody 100%
• 2006

  Cartilage, SOX9 and Notch signals in chondrogenesis

  Hardingham, T. E., Oldershaw, R. A. & Tew, S. R., Oct 2006, In: Journal of Anatomy. 209, 4, p. 469-480 11 p.

  Research output: Contribution to journal › Article › peer-review

  • Notch 100%
  • Chondrocyte 100%
  • SOX9 100%
  • Chondrogenesis 100%
  • Retrovirus 100%

• Raised chondroitin sulfate epitopes and hyaluronan in serum from rheumatoid arthritis and osteoarthritis patients

  Pothacharoen, P., Teekachunhatean, S., Louthrenoo, W., Yingsung, W., Ong-Chai, S., Hardingham, T. & Kongtawelert, P., Mar 2006, In: Osteoarthritis and Cartilage. 14, 3, p. 299-301 2 p.

  Research output: Contribution to journal › Article › peer-review

  • Hyaluronan 100%
  • Chondroitin Sulfate 100%
  • Restylane 100%
  • Inflammatory Arthritis 100%
  • Epitope 100%

• Raised serum chondroitin sulfate epitope level in ovarian epithelial cancer

  Pothacharoen, P., Siriaunkgul, S., Ong-Chai, S., Supabandhu, J., Kumja, P., Wanaphirak, C., Sugahara, K., Hardingham, T. & Kongtawelert, P., Oct 2006, In: Journal of Biochemistry. 140, 4, p. 517-524 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Chondroitin Sulfate 100%
  • Hyaluronan 87%
  • Restylane 87%
  • Ovary Disease 25%
  • Binding Protein 12%

• Regulation of SOX9 mRNA in human articular chondrocytes involving p38 MAPK activation and mRNA stabilization

  Tew, S. R. & Hardingham, T. E., 22 Dec 2006, In: Journal of Biological Chemistry. 281, 51, p. 39471-39479 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Mitogen-Activated Protein Kinase 100%
  • SOX9 100%
  • Chondrocyte 100%
  • Actin 60%
  • Stress Fiber 60%

• Solution structure and dynamics of cartilage aggrecan

  Papagiannopoulos, A., Waigh, T. A., Hardingham, T. & Heinrich, M., Jul 2006, In: Biomacromolecules. 7, 7, p. 2162-2172 10 p.

  Research output: Contribution to journal › Article › peer-review

  • Neutron Scattering 100%
  • Particle Tracking 100%
  • Aggrecan 100%
  • Solution Structure 100%
  • Intrinsic Viscosity 100%

• The matrix-forming phenotype of cultured human meniscus cells is enhanced after culture with fibroblast growth factor 2 and is further stimulated by hypoxia

  Adesida, A. B., Grady, L. M., Khan, W. S. & Hardingham, T. E., 17 Mar 2006, In: Arthritis Research and Therapy. 8, 3, R61.

  Research output: Contribution to journal › Article › peer-review

  • Basic Fibroblast Growth Factor 100%
  • Cell Aggregation 50%
  • Type II Collagen 33%
  • Proteoglycan 33%
  • Gene Expression 33%
• 2005

  Human meniscus cells in 3D pellet culture under hypoxia are chondrogenic: this is accompanied by upregulation of prolyl 4-hydroxylase type 1.

  Adesida, A., Tweats, L., Millwards-Sadler, J., Salter, D. & Hardingham, T., 2005, In: International Journal of Experimental Pathology. 86

  Research output: Contribution to journal › Article › peer-review

• Retroviral transduction with SOX9 enhances re-expression of the chondrocyte phenotype in passaged osteoarthritic human articular chondrocytes

  Tew, S. R., Li, Y., Pothancharoen, P., Tweats, L. M., Hawkins, R. E. & Hardingham, T. E., Jan 2005, In: Osteoarthritis and Cartilage. 13, 1, p. 80-89 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Chondrocyte 100%
  • SOX9 100%
  • Dermal Fibroblast 10%
  • Growth Factor 10%
  • Transcription Factor 10%

• Stimulation of cartilage matrix synthesis by human articular chondrocytes using SOX9 transfection and hypoxic culture conditions.

  Tew, S., Li, Y., Tweats, L., Hawkins, R. & Hardingham, T., 2005, In: International Journal of Experimental Pathology. 86

  Research output: Contribution to journal › Article › peer-review

• The putative role of the notch ligand, jagged 1, in the mediation of the early events of human mesenchymal stem cell chondrogenesis.

  Oldershaw, R., Murdoch, A., Brennan, K. & Hardingham, T., 2005, In: International Journal of Experimental Pathology. 86

  Research output: Contribution to journal › Article › peer-review
• 2004

  Alternative Splicing in the Aggrecan G3 Domain Influences Binding Interactions with Tenascin-C and Other Extracellular Matrix Proteins

  Day, J. M., Olin, A. I., Murdoch, A. D., Canfield, A., Sasaki, T., Timpl, R., Hardingham, T. E. & Aspberg, A., 26 Mar 2004, In: Journal of Biological Chemistry. 279, 13, p. 12511-12518 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Tenascin-R 100%

• Investigating the role of heparan sulfate proteoglycans in hereditary multiple exostoses (HME) tumourigenesis.

  Scholefield, Z., Hassell, J., West, L., Govindraj, P., Hecht, J., Rushton, G., Oldershaw, R., Hardingham, T., Merry, C. & Gallagher, J., 2004, In: International Journal of Experimental Pathology. 85, p. A73-A74

  Research output: Contribution to journal › Article › peer-review

• Transduction of Passaged Human Articular Chondrocytes with Adenoviral, Retroviral, and Lentiviral Vectors and the Effects of Enhanced Expression of SOX9

  Ying, L., Tew, S. R., Russell, A. M., Gonzalez, K. R., Hardingham, T. E. & Hawkins, R. E., Mar 2004, In: Tissue Engineering. 10, 3-4, p. 575-584 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Lentivirus Vector 100%
  • Retrovirus Vector 100%
  • Retrovirus 25%
  • Platelet Derived Growth Factor BB 12%
  • Cartilage Matrix 12%
• 2003

  Calcium-dependent protein interactions in MUC5B provide reversible cross-links in salivary mucus

  Raynal, B. D. E., Hardingham, T. E., Sheehan, J. K. & Thornton, D. J., 1 Aug 2003, In: Journal of Biological Chemistry. 278, 31, p. 28703-28710 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Positive Ion Electrospray 100%