Tim Hardingham, BSc, PhD, DSc

Search results

• 2020

  Generation of Human Induced Pluripotent Stem Cells from Anterior Cruciate Ligament

  Woods, S., Bates, N., Dunn, S. L., Serracino‐Inglott, F., Hardingham, T. E. & Kimber, S. J., 25 Jan 2020, In: Journal of Orthopaedic Research. 38, 1

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • Cells 100%
  • Anterior Cruciate Ligament 100%
  • Ligament 57%
  • Induced Pluripotent Stem Cell 42%
  • Fibroblast 42%
• 2019

  Enhanced chondrogenesis from human embryonic stem cells

  Wang, T., Nimkingratana, P., Smith, C. A., Cheng, A., Hardingham, T. & Kimber, S., 9 Jul 2019, (E-pub ahead of print) In: Stem Cell Research.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • Embryonic Stem Cell 100%
  • Gene Expression 33%
  • Type II Collagen 33%
  • Electric Potential 16%
  • Nested Gene 16%

• Identification of long non-coding RNAs expressed in knee and hip osteoarthritic cartilage

  Ajekigbe, B., Cheung, K., Xu, Y., Skelton, A. J., Panagiotopoulos, A., Soul, J., Hardingham, T. E., Deehan, D. J., Barter, M. J. & Young, D. A., 2019, In: Osteoarthritis and Cartilage.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • Non-Coding RNA 100%
  • Transcriptome 71%
  • RNA Sequencing 42%
  • Gene Expression 14%
  • Homeostasis 14%

• SkeletalVis: an exploration and meta-analysis data portal of cross-species skeletal transcriptomics data

  Soul, J., Hardingham, T., Boot-Handford, R. & Schwartz, J-M., Jul 2019, In: Bioinformatics. 35, p. 2283-2290

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • Datum 100%
  • Experiment 57%
  • Exploration 42%
  • Signature 42%
  • Exploratory Behavior 42%
• 2018

  Stratification of knee osteoarthritis: two major patient subgroups identified by genome wide expression analysis of articular cartilage.

  Soul, J., Dunn, S., Anand, S., Serracino-Inglott, F., Schwartz, J-M., Boot-Handford, R. & Hardingham, T., Mar 2018, In: Annals of the rheumatic diseases. 77, p. 423-430

  Research output: Contribution to journal › Article › peer-review

  Open Access
• 2016

  Gene expression changes in damaged osteoarthritic cartilage identify a signature of non-chondrogenic and mechanical responses

  Dunn, S., Soul, J., Anand, S., Schwartz, J-M., Boot-Handford, R. & Hardingham, T., Aug 2016, In: Osteoarthritis and Cartilage. 24, 8, p. 1431-1440 10 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • Cartilage 100%
  • Nested Gene 62%
  • Gene 50%
  • RNA Sequencing 50%
  • Transcription Factor 25%

• PhenomeScape: a Cytoscape app to identify differentially regulated sub-networks using known disease associations

  Soul, J., Dunn, S., Hardingham, T., Boot-Handford, R. & Schwartz, J-M., 15 Dec 2016, In: Bioinformatics (Oxford, England). 32, 24, p. 3847-9

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • Subnetwork 100%
  • Gene 50%
  • Phenotype 50%
  • Networks 25%
  • User 25%
• 2015

  PhenomeExpress: A refined network analysis of expression datasets by inclusion of known disease phenotypes

  Soul, J., Hardingham, T., Boot-Handford, R. & Schwartz, J-M., 29 Jan 2015, In: Scientific Reports. 5, 8117.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  • Phenotype 100%
  • Gene 14%
  • Mouse 14%
  • Protein Protein Interaction 14%
  • Biological Product 14%
• 2014

  Assembly of the respiratory Mucin MUC5B a new model for a gel-forming Mucin

  Ridley, C., Kouvatsos, N., Raynal, B. D., Howard, M., Collins, R. F., Desseyn, J. L., Jowitt, T. A., Baldock, C., Davis, C. W., Hardingham, T. E. & Thornton, D. J., 6 Jun 2014, In: Journal of Biological Chemistry. 289, 23, p. 16409-16420 11 p.

  Research output: Contribution to journal › Article › peer-review

  • MUC5B 100%
  • Polymerization 30%
  • Disulfide 30%
  • Cross-Link 20%
  • Calcium Binding 20%

• Cartilage Repair Using Human Embryonic Stem Cell-Derived Chondroprogenitors.

  Cheng, A., Kapacee, Z., Peng, J., Lu, S., Lucas, R. J., Hardingham, T. E. & Kimber, S. J., 1 Oct 2014, In: Stem Cells Translational Medicine. 3, 11, p. 1287-1294

  Research output: Contribution to journal › Article › peer-review

  • Cells 100%
  • Human Embryonic Stem Cell 88%
  • Embryonic Stem Cell 88%
  • Cartilage 55%
  • Culture 33%

• Generating Cartilage Repair from Pluripotent Stem Cells

  Cheng, A., Hardingham, T. & Kimber, SJ., 2014, In: Tissue Engineering. Part B. Reviews . 20, 4, p. 257-266 9 p.

  Research output: Contribution to journal › Article › peer-review
• 2010

  A chemically-defined protocol for generating chondrocytes from human embryonic stem cells.

  Oldershaw, RA., Baxter, MA., Lowe, ET., Bates, N., Grady, LM., Soncin, F., Brison, DR., Hardingham, TE. & Kimber, SJ., 2010, In: Nature biotechnology. 28, 11

  Research output: Contribution to journal › Article › peer-review

  • Human Embryonic Stem Cell 100%
  • Embryonic Stem Cell 100%
  • Chondrocyte 66%
  • Differentiation 66%
  • Cell Differentiation 33%

• Directed differentiation of human embryonic stem cells toward chondrocytes

  Oldershaw, R. A., Baxter, M. A., Lowe, E. T., Bates, N., Grady, L. M., Soncin, F., Brison, D. R., Hardingham, T. E. & Kimber, S. J., Nov 2010, In: Nature biotechnology. 28, 11, p. 1187-1194 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Cells 100%
  • Chondrocyte 100%
  • Differentiation 100%
  • Human Embryonic Stem Cell 100%
  • Embryonic Stem Cell 100%

• Fell-Muir lecture: Cartilage 2010 - The known unknowns

  Hardingham, T. E., Jun 2010, In: International Journal of Experimental Pathology. 91, 3, p. 203-209 6 p.

  Research output: Contribution to journal › Article › peer-review

  • Tissue 100%
  • Progress 100%
  • Tissues 100%
  • Comprehension 100%
  • Cartilage 100%

• Gene expression in human chondrocytes in late OA is changed in both fibrillated and intact cartilages without evidence of generalised chondrocyte hypertrophy

  Brew, CJ., Clegg, PD., Boot-Handford, R., Andrews, TE. & Hardingham, T., 2010, In: Annals Rheum Dis. 467

  Research output: Contribution to journal › Article › peer-review

• Gene expression in human chondrocytes in late osteoarthritis is changed in both fibrillated and intact cartilage without evidence of generalised chondrocyte hypertrophy

  Brew, C. J., Clegg, P. D., Boot-Handford, R. P., Andrew, J. G. & Hardingham, T., Jan 2010, In: Annals of the rheumatic diseases. 69, 1, p. 234-240 6 p.

  Research output: Contribution to journal › Article › peer-review

  • Gene Expression 100%
  • Chondrocyte 80%
  • Cartilage 80%
  • Age 20%
  • Joint 20%

• Hardingham T (2010) Cell and Tissue-based Approaches for Cartilage Repair

  Hardingham, T., 2010, In: ALTERNATIVES TO LABORATORY ANIMALS. 38, p. 35-39

  Research output: Contribution to journal › Article › peer-review

• Notch signaling during chondrogenesis of human bone marrow stem cells

  Oldershaw, R. A. & Hardingham, T. E., Feb 2010, In: Bone. 46, 2, p. 286-293 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Notch 100%
  • Chondrogenesis 90%
  • Notch Signaling 70%
  • Notch Signaling Pathway 70%
  • Bone 60%

• Order within disorder: Aggrecan chondroitin sulphate-attachment region provides new structural insights into protein sequences classified as disordered

  Jowitt, T. A., Murdoch, A. D., Baldock, C., Berry, R., Day, J. M. & Hardingham, T. E., Dec 2010, In: Proteins: Structure, Function and Bioinformatics. 78, 16, p. 3317-3327 10 p.

  Research output: Contribution to journal › Article › peer-review

  • Chondroitin Sulfate 100%
  • Peptide 83%
  • Aggrecan 83%
  • Protein 66%
  • Protein Secondary Structure 50%

• The potential of stem cells in the treatment of knee cartilage defects

  Khan, W. S., Johnson, D. S. & Hardingham, T. E., Dec 2010, In: The Knee. 17, 6, p. 369-374 5 p.

  Research output: Contribution to journal › Article › peer-review

  • Cartilage 100%
  • Cells 83%
  • Stem Cell 50%
  • Therapeutic Procedure 33%
  • Mesenchymal Stem Cell 33%
• 2009

  Hyperosmolarity regulates SOX9 mRNA posttranscriptionally in human articular chondrocytes

  Tew, S. R., Peffers, M. J., McKay, T. R., Lowe, E. T., Khan, W. S., Hardingham, T. E. & Clegg, P. D., 2009, In: American Journal of Physiology: Cell Physiology. 297, 4, p. C898-C906

  Research output: Contribution to journal › Article › peer-review

  • SOX9 100%
  • Chondrocyte 80%
  • Messenger RNA 80%
  • p38 Mitogen-Activated Protein Kinase 60%
  • Hyperosmolarity 30%

• Notch signaling during chondrogenesis of bone marrow stem cells

  Hardingham, T. & Oldershaw, RA., 2009, In: Bone. 350

  Research output: Contribution to journal › Article › peer-review

• The epitope characterisation and the osteogenic differentiation potential of human fat pad-derived stem cells is maintained with ageing in later life

  Khan, W. S., Adesida, A. B., Tew, S. R., Andrew, J. G. & Hardingham, T. E., Feb 2009, In: Injury. 40, 2, p. 150-157 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Electric Potential 100%
  • Cells 71%
  • Stem Cell 71%
  • Fat Pad 71%
  • Aging 57%

• The influence of donor and hypoxic conditions on the assembly of cartilage matrix by osteoarthritic human articular chondrocytes on Hyalograft matrices

  Katopodi, T., Tew, S. R., Clegg, P. D. & Hardingham, T. E., Feb 2009, In: Biomaterials. 30, 4, p. 535-540 5 p.

  Research output: Contribution to journal › Article › peer-review

  • Chondrocyte 100%
  • Cartilage 50%
  • Oxygen 50%
  • Donor 50%
  • Cartilage Matrix 50%
• 2008

  Cellular methods in cartilage research: Primary human chondrocytes in culture and chondrogenesis in human bone marrow stem cells

  Tew, S. R., Murdoch, A. D., Rauchenberg, R. P. & Hardingham, T. E., May 2008, In: Methods. 45, 1, p. 2-9 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Cartilage 100%
  • Chondrocyte 66%
  • Culture 66%
  • In Vitro 33%
  • Cells 33%

• Extracellular matrix and pathogenic mechanisms in osteoarthritis

  Hardingham, T., Jan 2008, In: Current Rheumatology Reports. 10, 1, p. 30-36 6 p.

  Research output: Contribution to journal › Article › peer-review

  • Cartilage 100%
  • Osteoarthritis 80%
  • Extracellular Matrix 40%
  • Aggrecan 40%
  • Joint Degeneration 40%

• Human infrapatellar fat pad-derived stem cells express the pericyte marker 3G5 and show enhanced chondrogenesis after expansion in fibroblast growth factor-2

  Khan, W. S., Tew, S. R., Adesida, A. B. & Hardingham, T. E., 3 Jul 2008, In: Arthritis Research and Therapy. 10, 4, R74.

  Research output: Contribution to journal › Article › peer-review

  • Cells 100%
  • Infrapatellar Fat Pad 66%
  • Fibroblast Growth Factor 2 50%
  • Differentiation 33%
  • Tissues 25%

• Hyaluronan binding to Link module of TSG-6 and to G1 domain of aggrecan is differently regulated by pH

  Boon, C. H., Gribbon, P. M., Day, A. J. & Hardingham, T. E., 21 Nov 2008, In: Journal of Biological Chemistry. 283, 47, p. 32294-32301 7 p.

  Research output: Contribution to journal › Article › peer-review

  • pH 100%
  • Hyaluronan 53%
  • TSG-6 30%
  • Binding Affinity 30%
  • Aggrecan 30%

• Notch signaling through Jagged-1 is necessary to initiate chondrogenesis in human bone marrow stromal cells but must be switched off to complete chondrogenesis

  Oldershaw, R. A., Tew, S. R., Russell, A. M., Meade, K., Hawkins, R., McKay, T. R., Brennan, K. R. & Hardingham, T. E., Mar 2008, In: Stem Cells. 26, 3, p. 666-674 8 p.

  Research output: Contribution to journal › Article › peer-review

  • Chondrogenesis 100%
  • Notch Signaling 60%
  • Notch 60%
  • Protein Jagged 1 60%
  • Notch Signaling Pathway 60%

• SOX9 transduction increases chondroitin sulfate synthesis in cultured human articular chondrocytes without altering glycosyltransferase and sulfotransferase transcription

  Tew, S. R., Pothacharoen, P., Katopodi, T. & Hardingham, T. E., 1 Sep 2008, In: Biochemical Journal. 414, 2, p. 231-236 5 p.

  Research output: Contribution to journal › Article › peer-review

  • SOX9 100%
  • Chondroitin Sulfate 70%
  • Glycosaminoglycan 60%
  • Enzyme 40%
  • Lysozyme 30%
• 2007

  Chondrogenic differentiation of human bone marrow stem cells in transwell cultures: Generation of scaffold-free cartilage

  Murdoch, A. D., Grady, L. M., Ablett, M. P., Katopodi, T., Meadows, R. S. & Hardingham, T. E., Nov 2007, In: Stem Cells. 25, 11, p. 2786-2796 10 p.

  Research output: Contribution to journal › Article › peer-review

  • Bone 100%
  • Growth 50%
  • Electric Potential 33%
  • Membrane 16%
  • Nested Gene 16%

• Differential cartilaginous tissue formation by human synovial membrane, fat pad, meniscus cells and articular chondrocytes

  Marsano, A., Millward-Sadler, S. J., Salter, D. M., Adesida, A., Hardingham, T., Tognana, E., Kon, E., Chiari-Grisar, C., Nehrer, S., Jakob, M. & Martin, I., Jan 2007, In: Osteoarthritis and Cartilage. 15, 1, p. 48-58 10 p.

  Research output: Contribution to journal › Article › peer-review

  • Tissues 100%
  • Chondrocyte 100%
  • Cells 66%
  • Synovial Membrane 66%
  • Glycosaminoglycan 66%

• Human meniscus cells express hypoxia inducible factor-1alpha and increased. SOX9 in response to low oxygen tension in cell aggregate culture

  Adesida, AB., Grady, LM., Khan, WS., Millward-Sadler, SJ., Salter, DM. & Hardingham, TE., 2007, In: Arthritis Research and Therapy. 9, 4

  Research output: Contribution to journal › Article › peer-review

• Hypoxic conditions increase hypoxia-inducible transcription factor 2alpha and enhance chondrogenesis in stem cells from the infrapatellar fat pad of osteoarthritis patients.

  Khan, WS., Adesida, AB. & Hardingham, TE., 2007, In: Arthritis Research and Therapy. 9, 3

  Research output: Contribution to journal › Article › peer-review

• SOX9 transduction of a human chondrocytic cell line identifies novel genes regulated in primary human chondrocytes and in osteoarthritis

  Tew, S. R., Clegg, P. D., Brew, C. J., Redmond, C. M. & Hardingham, T. E., 12 Oct 2007, In: Arthritis Research and Therapy. 9, 5, R107.

  Research output: Contribution to journal › Article › peer-review

  • SOX9 100%
  • Nested Gene 80%
  • Sample 10%
  • Gene Expression 10%
  • Age 10%

• Two related but distinct chondroitin sulfate mimetope octasaccharide sequences recognized by monoclonal antibody WF6

  Pothacharoen, P., Kalayanamitra, K., Deepa, S. S., Fukui, S., Hattori, T., Fukushima, N., Hardingham, T., Kongtawelert, P. & Sugahara, K., 30 Nov 2007, In: Journal of Biological Chemistry. 282, 48, p. 35232-35246 14 p.

  Research output: Contribution to journal › Article › peer-review

  • Chondroitin Sulfate 100%
  • Oligosaccharide 66%
  • Shark 33%
  • Monoclonal Antibody 33%
  • Lysozyme 16%
• 2006

  Cartilage, SOX9 and Notch signals in chondrogenesis

  Hardingham, T. E., Oldershaw, R. A. & Tew, S. R., Oct 2006, In: Journal of Anatomy. 209, 4, p. 469-480 11 p.

  Research output: Contribution to journal › Article › peer-review

  • Chondrocyte 100%
  • Cartilage 100%
  • Cells 66%
  • Culture 66%
  • Mesenchymal Stem Cell 66%

• Raised chondroitin sulfate epitopes and hyaluronan in serum from rheumatoid arthritis and osteoarthritis patients

  Pothacharoen, P., Teekachunhatean, S., Louthrenoo, W., Yingsung, W., Ong-Chai, S., Hardingham, T. & Kongtawelert, P., Mar 2006, In: Osteoarthritis and Cartilage. 14, 3, p. 299-301 2 p.

  Research output: Contribution to journal › Article › peer-review

  • Chondroitin Sulfate 100%
  • Hyaluronan 83%
  • Sample 16%
  • Contrast 16%
  • Association 16%

• Raised serum chondroitin sulfate epitope level in ovarian epithelial cancer

  Pothacharoen, P., Siriaunkgul, S., Ong-Chai, S., Supabandhu, J., Kumja, P., Wanaphirak, C., Sugahara, K., Hardingham, T. & Kongtawelert, P., Oct 2006, In: Journal of Biochemistry. 140, 4, p. 517-524 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Chondroitin Sulfate 100%
  • Hyaluronan 87%
  • Development 12%
  • Binding Protein 12%
  • Metabolic Pathway 12%

• Regulation of SOX9 mRNA in human articular chondrocytes involving p38 MAPK activation and mRNA stabilization

  Tew, S. R. & Hardingham, T. E., 22 Dec 2006, In: Journal of Biological Chemistry. 281, 51, p. 39471-39479 8 p.

  Research output: Contribution to journal › Article › peer-review

  • SOX9 100%
  • Messenger RNA 75%
  • p38 Mitogen-Activated Protein Kinase 50%
  • Actin 37%
  • Stress Fiber 37%

• Solution structure and dynamics of cartilage aggrecan

  Papagiannopoulos, A., Waigh, T. A., Hardingham, T. & Heinrich, M., Jul 2006, In: Biomacromolecules. 7, 7, p. 2162-2172 10 p.

  Research output: Contribution to journal › Article › peer-review

  • Aggrecan 100%
  • Dynamics 66%
  • Solution and Solubility 33%
  • Spectroscopy 33%
  • Videorecording 33%

• The matrix-forming phenotype of cultured human meniscus cells is enhanced after culture with fibroblast growth factor 2 and is further stimulated by hypoxia

  Adesida, A. B., Grady, L. M., Khan, W. S. & Hardingham, T. E., 17 Mar 2006, In: Arthritis Research and Therapy. 8, 3, R61.

  Research output: Contribution to journal › Article › peer-review

  • Fibroblast Growth Factor 100%
  • Cell Aggregation 50%
  • Gene Expression 33%
  • Proteoglycan 33%
  • Oxygen Tension 33%
• 2005

  Human meniscus cells in 3D pellet culture under hypoxia are chondrogenic: this is accompanied by upregulation of prolyl 4-hydroxylase type 1.

  Adesida, A., Tweats, L., Millwards-Sadler, J., Salter, D. & Hardingham, TE., 2005, In: International Journal of Experimental Pathology. 86

  Research output: Contribution to journal › Article › peer-review

• Retroviral transduction with SOX9 enhances re-expression of the chondrocyte phenotype in passaged osteoarthritic human articular chondrocytes

  Tew, S. R., Li, Y., Pothancharoen, P., Tweats, L. M., Hawkins, R. E. & Hardingham, T. E., Jan 2005, In: Osteoarthritis and Cartilage. 13, 1, p. 80-89 9 p.

  Research output: Contribution to journal › Article › peer-review

  • Chondrocyte 100%
  • SOX9 80%
  • Cells 60%
  • Joint 50%
  • Phenotype 40%

• Stimulation of cartilage matrix synthesis by human articular chondrocytes using SOX9 transfection and hypoxic culture conditions.

  Tew, SR., Li, Y., Tweats, LM., Hawkins, RE. & Hardingham, TE., 2005, In: International Journal of Experimental Pathology. 86

  Research output: Contribution to journal › Article › peer-review

• The putative role of the notch ligand, jagged 1, in the mediation of the early events of human mesenchymal stem cell chondrogenesis.

  Oldershaw, R., Murdoch, A., Brennan, K. & Hardingham, TE., 2005, In: International Journal of Experimental Pathology. 86

  Research output: Contribution to journal › Article › peer-review
• 2004

  Alternative Splicing in the Aggrecan G3 Domain Influences Binding Interactions with Tenascin-C and Other Extracellular Matrix Proteins

  Day, J. M., Olin, A. I., Murdoch, A. D., Canfield, A., Sasaki, T., Timpl, R., Hardingham, T. E. & Aspberg, A., 26 Mar 2004, In: Journal of Biological Chemistry. 279, 13, p. 12511-12518 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Lectin 100%
  • Aggrecan 100%
  • Proteoglycan 60%
  • Epidermal Growth Factor 60%
  • Fibulin 60%

• Investigating the role of heparan sulfate proteoglycans in hereditary multiple exostoses (HME) tumourigenesis.

  Scholefield, ZM., Hassell, JR., West, L., Govindraj, P., Hecht, JT., Rushton, G., Oldershaw, R., Hardingham, T., Merry, CLR. & Gallagher, JT., 2004, In: International Journal of Experimental Pathology. 85, p. A73-A74

  Research output: Contribution to journal › Article › peer-review

• Transduction of Passaged Human Articular Chondrocytes with Adenoviral, Retroviral, and Lentiviral Vectors and the Effects of Enhanced Expression of SOX9

  Ying, L., Tew, S. R., Russell, A. M., Gonzalez, K. R., Hardingham, T. E. & Hawkins, R. E., Mar 2004, In: Tissue Engineering. 10, 3-4, p. 575-584 9 p.

  Research output: Contribution to journal › Article › peer-review

  • SOX9 100%
  • Retrovirus Vector 100%
  • Adenovirus Vector 100%
  • Lentivirus Vector 75%
  • Monolayer Culture 75%
• 2003

  Calcium-dependent protein interactions in MUC5B provide reversible cross-links in salivary mucus

  Raynal, B. D. E., Hardingham, T. E., Sheehan, J. K. & Thornton, D. J., 1 Aug 2003, In: Journal of Biological Chemistry. 278, 31, p. 28703-28710 7 p.

  Research output: Contribution to journal › Article › peer-review

  • Calcium 100%
  • Facilitated Diffusion 33%
  • Protein 33%
  • MUC5B 25%
  • Tracer 25%