Research output per year
Research output per year
Research output
- 41 Article
- 41 results
Search results
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2020
Covalent attachment of active enzymes to upconversion phosphors allows ratiometric detection of substrates
Natrajan, L., Hay, S., Burgess, L., Harvey, P. & Jones, A., 31 May 2020, In: Chemistry – A European Journal.Research output: Contribution to journal › Article › peer-review
Open AccessFile32 Downloads (Pure) -
Evaluating spectral overlap with the degree of quenching in UCP luminescence energy transfer systems
Burgess, L., Wilson, H., Jones, A., Hay, S. & Natrajan, L., 7 Jul 2020, (Accepted/In press) In: Methods and Applications in Fluorescence.Research output: Contribution to journal › Article › peer-review
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2019
Equatorial active site compaction and electrostatic reorganization in catechol-O-methyltransferase
Czarnota, S., Johannissen, L. O., Baxter, N. J., Rummel, F., Wilson, A., Cliff, M. J., Levy, C. W., Scrutton, N. S., Waltho, J. P. & Hay, S., 2019, In: ACS Catalysis. 9, 5, p. 4394-4401 8 p.Research output: Contribution to journal › Article › peer-review
Open Access -
2018
Non-equivalence of second sphere ‘non-catalytic’ residues in pentaerythritol tetranitrate reductase in relation to local dynamics linked to H-transfer in reactions with NADH and NADPH coenzymes
Iorgu, A. I., Baxter, N. J., Cliff, M. J., Levy, C., Waltho, J. P., Hay, S. & Scrutton, N. S., 7 Dec 2018, In: ACS Catalysis. 8, 12, p. 11589-11599 11 p.Research output: Contribution to journal › Article › peer-review
Open Access -
2017
A perspective on conformational control of electron transfer in nitric oxide synthases
Hedison, T., Hay, S. & Scrutton, N., 28 Feb 2017, In: Nitric Oxide: Biology and Chemistry. 63, p. 61-67 7 p.Research output: Contribution to journal › Article › peer-review
Open Access -
Convergence of Theory and Experiment on the Role of Preorganization, Quantum Tunneling, and Enzyme Motions into Flavoenzyme-Catalyzed Hydride Transfer
Delgado, M., Gorlich, S., Longbotham, J. E., Scrutton, N., Hay, S., Moliner, V. & Tuñón, I., 2017, In: ACS Catalysis. 7, p. 3190–3198Research output: Contribution to journal › Article › peer-review
Open AccessFile150 Downloads (Pure) -
Decoupled Associative and Dissociative Processesin Strong yet Highly Dynamic Host-Guest Complexes
Appel, E. A., Biedermann, F., Hoogland, D., del Barrio, J., Driscoll, M. D., Hay, S., Wales, D. J. & Scherman, O. A., 29 Jun 2017, In: Journal of the American Chemical Society. 139, 37, p. 12985-12993Research output: Contribution to journal › Article › peer-review
Open AccessFile239 Downloads (Pure) -
Expanding the scope of biomolecule monitoring with ratiometric signaling from rare-earth upconverting phosphors
Natrajan, L., Hay, S., Jones, A., Harvey, P., Oakland, C., Burgess, L. & Andrews, M., 3 Aug 2017, In: European Journal of Inorganic Chemistry. 10 p.Research output: Contribution to journal › Article › peer-review
Open AccessFile93 Downloads (Pure) -
2016
Applications of Microbial Cytochrome P450 Enzymes in Biotechnology and Synthetic Biology
Girvan, H. & Munro, A., Apr 2016, In: Current Opinion in Chemical Biology. 31, p. 136-145 10 p.Research output: Contribution to journal › Article › peer-review
Open Access -
Carboxylesterase converts Amplex red to resorufin: Implications for mitochondrial H2O2 release assays.
Miwa, S., Treumann, A., Bell, A., Vistoli, G., Nelson, G., Hay, S. & von Zglinicki, T., 1 Jan 2016, In: Free radical biology & medicine. 90, p. 173-183Research output: Contribution to journal › Article › peer-review
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Correlating calmodulin landscapes with chemical catalysis in neuronal nitric oxide synthase using time-resolved FRET and a 5- deazaflavin thermodynamic trap.
Hedison, T., Leferink, N., Hay, S. & Scrutton, N., 5 Aug 2016, In: ACS Catalysis. 6, 8, p. 5170–5180 11 p.Research output: Contribution to journal › Article › peer-review
Open Access -
Dual transcriptional-translational cascade permits cellular level tuneable expression control.
Morra, R., Shankar, J., Robinson, C., Halliwell, S., Butler, L., Upton, M., Hay, S., Micklefield, J. & Dixon, N., 18 Feb 2016, In: Nucleic acids research. 44, 3, e21.Research output: Contribution to journal › Article › peer-review
Open Access -
Ground-state Destabilization by Phe-448 and Phe-449 Contributes to Tyrosine Phenol-lyase Catalysis
Phillips, R. S., Vita, A., Spivey, J. B., Rudloff, A. P., Driscoll, M. & Hay, S., 2016, In: ACS Catalysis. 6, 10, p. 6770-6779 9 p.Research output: Contribution to journal › Article › peer-review
Open AccessFile129 Downloads (Pure) -
Time course analysis of enzyme-catalyzed DNA polymerization
Rentergent, J., Driscoll, M. & Hay, S., 2016, In: Biochemistry. 55, 39, p. 5622–5634 13 p.Research output: Contribution to journal › Article › peer-review
Open AccessFile293 Downloads (Pure) -
Untangling heavy protein and cofactor isotope effects on enzyme catalyzed hydride transfer
Longbotham, J. E., Hardman, S., Gorlich, S., Scrutton, N. & Hay, S., 27 Sep 2016, In: Journal of the American Chemical Society. 138, 41, p. 13693–13699Research output: Contribution to journal › Article › peer-review
Open AccessFile320 Downloads (Pure) -
2015
Does the pressure dependence of kinetic isotope effects report usefully on dynamics in enzyme H-transfer reactions?
Hoeven, R., Heyes, D. J., Hay, S. & Scrutton, NS., 2015, In: The FEBS Journal. 282, 16, p. 3243-3255 12 p.Research output: Contribution to journal › Article › peer-review
Open Access -
Nuclear quantum tunnelling in enzymatic reactions - an enzymologist's perspective.
Johannissen, L., Hay, S. & Scrutton, NS., 18 Nov 2015, In: Physical Chemistry Chemical Physics. 17, 46, p. 30775-30782 8 p.Research output: Contribution to journal › Article › peer-review
Open Access -
Probing reversible chemistry in coenzyme B12-dependent ethanolamine ammonia lyase with kinetic isotope effects
Jones, A. R., Rentergent, J., Scrutton, NS. & Hay, S., 2015, In: Chemistry. 21, 24, p. 8826-8831 5 p.Research output: Contribution to journal › Article › peer-review
Open AccessFile82 Downloads (Pure) -
Real time analysis of conformational control in electron transfer reactions of human cytochrome P450 reductase with cytochrome c
Hedison, T. M., Hay, S. & Scrutton, N., 16 Sep 2015, In: The FEBS Journal. 282, 22, p. 4357–4375 19 p.Research output: Contribution to journal › Article › peer-review
Open Access -
Structure and Mechanism of a Viral Collagen Prolyl Hydroxylase
Longbotham, J. E., Levy, C., Johannissen, L. O., Tarhonskaya, H., Jiang, S., Loenarz, C., Flashman, E., Hay, S., Schofield, C. J. & Scrutton, N. S., 2015, In: Biochemistry. 54, 39, p. 6093-6105 13 p.Research output: Contribution to journal › Article › peer-review
Open Access -
Towards the free energy landscape for catalysis in mammalian nitric oxide synthases
Leferink, N., Hay, S., Rigby, S. E. J. & Scrutton, NS., 2015, In: The FEBS Journal. 282, 16, p. 3016-3029 13 p.Research output: Contribution to journal › Article › peer-review
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2014
A quantitative fluorescence-based steady-state assay of DNA polymerase
Driscoll, M. D., Rentergent, J. & Hay, S., Apr 2014, In: FEBS Journal. 281, 8, p. 2042-2050 9 p.Research output: Contribution to journal › Article › peer-review
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Energy landscapes and catalysis in nitric-oxide synthase
Sobolewska-Stawiarz, A., Leferink, N. G. H., Fisher, K., Heyes, D. J., Hay, S., Rigby, S. E. J. & Scrutton, N. S., 25 Apr 2014, In: Journal of Biological Chemistry. 289, 17, p. 11725-11738 13 p.Research output: Contribution to journal › Article › peer-review
Open Access -
Practical aspects on the use of kinetic isotope effects as probes of flavoprotein enzyme mechanisms.
Pudney, CR., Hay, S., Scrutton, NS., Weber, S. (ed.) & Schleicher, E. (ed.), 2014, In: Methods in Molecular Biology. 1146, p. 161-175Research output: Contribution to journal › Article › peer-review
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Proton tunnelling and promoting vibrations during the oxidation of ascorbate by ferricyanide?
Kandathil, S. M., Driscoll, M. D., Dunn, R. V., Scrutton, N. S. & Hay, S., 14 Feb 2014, In: Physical Chemistry Chemical Physics. 16, 6, p. 2256-2259 3 p.Research output: Contribution to journal › Article › peer-review
Open Access -
Ratiometric detection of enzyme turnover and flavin reduction using rare-earth upconverting phosphors
Harvey, P., Oakland, C., Driscoll, M. D., Hay, S. & Natrajan, L. S., 14 Apr 2014, In: Dalton Transactions. 43, 14, p. 5265-5268 3 p.Research output: Contribution to journal › Article › peer-review
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2013
Dynamic, Electrostatic Model for the Generation and Control of High-Energy Radical Intermediates by a Coenzyme B12-Dependent Enzyme
Chen, Z. G., Zietek, M. A., Russell, H. J., Tait, S., Hay, S., Jones, A. R. & Scrutton, N. S., Sep 2013, In: ChemBioChem: a European journal of chemical biology . 14, 13, p. 1529-1533 4 p.Research output: Contribution to journal › Article › peer-review
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Enzymatic single-molecule kinetic isotope effects
Pudney, C. R., Lane, R. S. K., Fielding, A. J., Magennis, S. W., Hay, S. & Scrutton, N. S., 13 Mar 2013, In: Journal of the American Chemical Society. 135, 10, p. 3855-3864 9 p.Research output: Contribution to journal › Article › peer-review
Open AccessFile76 Downloads (Pure) -
Excited state dynamics can be used to probe donor-acceptor distances for H-tunneling reactions catalyzed by flavoproteins
Hardman, S. J. O., Pudney, C. R., Hay, S. & Scrutton, N. S., 3 Dec 2013, In: BIOPHYSICAL JOURNAL. 105, 11, p. 2549-2558 9 p.Research output: Contribution to journal › Article › peer-review
Open AccessFile40 Downloads (Pure) -
Fast protein motions are coupled to enzyme H-transfer reactions.
Pudney, C. R., Guerriero, A., Baxter, N. J., Johannissen, L. O., Waltho, J. P., Hay, S. & Scrutton, N. S., 20 Feb 2013, In: Journal of the American Chemical Society. 135, 7, p. 2512-2517 5 p.Research output: Contribution to journal › Article › peer-review
Open AccessFile29 Downloads (Pure) -
Modulation of ligand-heme reactivity by binding pocket residues demonstrated in cytochrome c' over the femtosecond-second temporal range
Russell, H. J., Hardman, S. J. O., Heyes, D. J., Hough, M. A., Greetham, G. M., Towrie, M., Hay, S. & Scrutton, N. S., Dec 2013, In: FEBS Journal. 280, 23, p. 6070-6082 12 p.Research output: Contribution to journal › Article › peer-review
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Relating localized protein motions to the reaction coordinate in coenzyme B12-dependent enzymes
Jones, A. R., Levy, C., Hay, S. & Scrutton, N. S., Jul 2013, In: FEBS Journal. 280, 13, p. 2997-3008 11 p.Research output: Contribution to journal › Article › peer-review
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2012
Evidence of preorganization in quinonoid intermediate formation from l -Trp in H463F mutant Escherichia coli tryptophan indole-lyase from effects of pressure and pH
Phillips, R. S., Kalu, U. & Hay, S., 21 Aug 2012, In: Biochemistry. 51, 33, p. 6527-6533 6 p.Research output: Contribution to journal › Article › peer-review
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Good vibrations in enzyme-catalysed reactions
Hay, S. & Scrutton, N. S., Mar 2012, In: Nature Chemistry. 4, 3, p. 161-168 7 p.Research output: Contribution to journal › Article › peer-review
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Preparation and photophysical properties of a caged kynurenine
Maitrani, C., Heyes, D. J., Hay, S., Arumugam, S., Popik, V. V. & Phillips, R. S., 15 Apr 2012, In: Bioorganic and Medicinal Chemistry Letters. 22, 8, p. 2734-2737 3 p.Research output: Contribution to journal › Article › peer-review
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Pressure effects on enzyme-catalyzed quantum tunneling events arise from protein-specific structural and dynamic changes
Hay, S., Johannissen, L. O., Hothi, P., Sutcliffe, M. J. & Scrutton, N. S., 13 Jun 2012, In: Journal of the American Chemical Society. 134, 23, p. 9749-9754 5 p.Research output: Contribution to journal › Article › peer-review
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Protein motions are coupled to the reaction chemistry in coenzyme B 12-dependent ethanolamine ammonia lyase
Russell, H. J., Jones, A., Hay, S., Greetham, G. M., Towrie, M. & Scrutton, N., 10 Sep 2012, In: Angewandte Chemie International Edition. 51, 37, p. 9306-9310 4 p.Research output: Contribution to journal › Article › peer-review
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Ultrafast infrared spectral fingerprints of Vitamin B12 and related cobalamins
Jones, A., Russell, H. J., Greetham, G. M., Towrie, M., Hay, S. & Scrutton, N., 21 May 2012, In: Journal of Physical Chemistry A. 116, 23, p. 5586-5594 9 p.Research output: Contribution to journal › Article › peer-review
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2011
Electrochemical and structural properties of a protein system designed to generate tyrosine pourbaix diagrams
Martínez-Rivera, M. C., Berry, B. W., Valentine, K. G., Westerlund, K., Hay, S. & Tommos, C., 9 Nov 2011, In: Journal of the American Chemical Society. 133, 44, p. 17786-17795 9 p.Research output: Contribution to journal › Article › peer-review
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Examining the importance of dynamics, barrier compression and hydrogen tunnelling in enzyme catalysed reactions
Hay, S. & Scrutton, N. S., 2011, In: Procedia Chemistry. 3, 1, p. 306-315 9 p.Research output: Contribution to journal › Article › peer-review
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Is there a dynamic protein contribution to the substrate trigger in coenzyme B 12-dependent ethanolamine ammonia lyase?
Jones, A. R., Hardman, S. J. O., Hay, S. & Scrutton, N. S., 11 Nov 2011, In: Angewandte Chemie - International Edition. 50, 46, p. 10843-10846 3 p.Research output: Contribution to journal › Article › peer-review