α-Calcitonin gene related peptide (α-CGRP) mediated lipid mobilization in 3T3-L1 adipocytes

Christopher S. Walker; Debbie L. Hay; Sandra M. Fitzpatrick; Garth Cooper; Kerry M. Loomes

doi:10.1016/j.peptides.2014.05.011

α-Calcitonin gene related peptide (α-CGRP) mediated lipid mobilization in 3T3-L1 adipocytes

Christopher S. Walker, Debbie L. Hay, Sandra M. Fitzpatrick, Garth Cooper, Kerry M. Loomes

University of Auckland

Research output: Contribution to journal › Article › peer-review

Abstract

The neuropeptide, α-calcitonin gene-related peptide (α-CGRP), is expressed from sensory nerves that innervate fat. However, how α-CGRP may act in adipose tissue is unclear. Using 3T3-L1 adipocytes we observed that rat α-CGRP (rα-CGRP) evoked either a biphasic or monophasic reduction in intracellular free fatty acid (FFA) content. cAMP production was always monophasic and occurred when FFA responses were absent. Taken together with the observed potencies, these findings suggest that adipose tissue is a physiological target for α-CGRP. However, uncoupling of the FFA and CGRP-signaling responses with increasing passage number limits 3T3-L1 adipocytes as a suitable cellular model. © 2014 Elsevier Inc.

Original language	English
Pages (from-to)	14-19
Number of pages	5
Journal	Peptides
Volume	58
DOIs	https://doi.org/10.1016/j.peptides.2014.05.011
Publication status	Published - 2014

Keywords

Adipose
cAMP
CGRP
Lipid
Lipolysis

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title = "α-Calcitonin gene related peptide (α-CGRP) mediated lipid mobilization in 3T3-L1 adipocytes",

abstract = "The neuropeptide, α-calcitonin gene-related peptide (α-CGRP), is expressed from sensory nerves that innervate fat. However, how α-CGRP may act in adipose tissue is unclear. Using 3T3-L1 adipocytes we observed that rat α-CGRP (rα-CGRP) evoked either a biphasic or monophasic reduction in intracellular free fatty acid (FFA) content. cAMP production was always monophasic and occurred when FFA responses were absent. Taken together with the observed potencies, these findings suggest that adipose tissue is a physiological target for α-CGRP. However, uncoupling of the FFA and CGRP-signaling responses with increasing passage number limits 3T3-L1 adipocytes as a suitable cellular model. {\textcopyright} 2014 Elsevier Inc.",

keywords = "Adipose, cAMP, CGRP, Lipid, Lipolysis",

author = "Walker, {Christopher S.} and Hay, {Debbie L.} and Fitzpatrick, {Sandra M.} and Garth Cooper and Loomes, {Kerry M.}",

year = "2014",

doi = "10.1016/j.peptides.2014.05.011",

language = "English",

volume = "58",

pages = "14--19",

journal = "Peptides",

issn = "1873-5169",

publisher = "Elsevier BV",

}

TY - JOUR

T1 - α-Calcitonin gene related peptide (α-CGRP) mediated lipid mobilization in 3T3-L1 adipocytes

AU - Walker, Christopher S.

AU - Hay, Debbie L.

AU - Fitzpatrick, Sandra M.

AU - Cooper, Garth

AU - Loomes, Kerry M.

PY - 2014

Y1 - 2014

N2 - The neuropeptide, α-calcitonin gene-related peptide (α-CGRP), is expressed from sensory nerves that innervate fat. However, how α-CGRP may act in adipose tissue is unclear. Using 3T3-L1 adipocytes we observed that rat α-CGRP (rα-CGRP) evoked either a biphasic or monophasic reduction in intracellular free fatty acid (FFA) content. cAMP production was always monophasic and occurred when FFA responses were absent. Taken together with the observed potencies, these findings suggest that adipose tissue is a physiological target for α-CGRP. However, uncoupling of the FFA and CGRP-signaling responses with increasing passage number limits 3T3-L1 adipocytes as a suitable cellular model. © 2014 Elsevier Inc.

AB - The neuropeptide, α-calcitonin gene-related peptide (α-CGRP), is expressed from sensory nerves that innervate fat. However, how α-CGRP may act in adipose tissue is unclear. Using 3T3-L1 adipocytes we observed that rat α-CGRP (rα-CGRP) evoked either a biphasic or monophasic reduction in intracellular free fatty acid (FFA) content. cAMP production was always monophasic and occurred when FFA responses were absent. Taken together with the observed potencies, these findings suggest that adipose tissue is a physiological target for α-CGRP. However, uncoupling of the FFA and CGRP-signaling responses with increasing passage number limits 3T3-L1 adipocytes as a suitable cellular model. © 2014 Elsevier Inc.

KW - Adipose

KW - cAMP

KW - CGRP

KW - Lipid

KW - Lipolysis

U2 - 10.1016/j.peptides.2014.05.011

DO - 10.1016/j.peptides.2014.05.011

M3 - Article

SN - 1873-5169

VL - 58

SP - 14

EP - 19

JO - Peptides

JF - Peptides

ER -

α-Calcitonin gene related peptide (α-CGRP) mediated lipid mobilization in 3T3-L1 adipocytes

Abstract

Keywords

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