1-Chloromethylpyrene: a reference skin sensitizer and genotoxin

J. Ashby, J. E. Gallagher, M. Kohan, H. Tinwell, I. Kimber, D. Paton, R. D. Callander, I. Chouroulinkov

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    1-Chloromethylpyrene (1-CMP) has been evaluated as a model mutagen and toxin related to the ultimate electrophiles derived from benzo[a]pyrene and 1-nitropyrene. It was mutagenic to Salmonella (> 100 pg/plate) an exceptionally reactive to DNA when assessed by the 32P-postlabelling technique. 1-CMP was inactive in a mouse bone micronucleus assay when administered by gavage, probably due to hydrolysis, whose kinetics have been studied (t 1 2 ∼ 23 min at 37°C). However, as expected, it was a potent skin toxin as determined by its activity as a mitogen to mouse skin and its contact allergenicity, as determined using the local lymph node proliferative assay. It is concluded that 1-CMP will probably be a potent human skin carcinogen and contact allergen. © 1990.
    Original languageEnglish
    Pages (from-to)281-289
    Number of pages8
    JournalMutation Research Letters
    Issue number4
    Publication statusPublished - Apr 1990


    • Bone-marrow micronuclei
    • DNA adducts
    • I-Chloromethylpyrene
    • Skin sensitization


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