1-Chloromethylpyrene: a reference skin sensitizer and genotoxin

J. Ashby; J. E. Gallagher; M. Kohan; H. Tinwell; I. Kimber; D. Paton; R. D. Callander; I. Chouroulinkov

1-Chloromethylpyrene: a reference skin sensitizer and genotoxin

J. Ashby, J. E. Gallagher, M. Kohan, H. Tinwell, I. Kimber, D. Paton, R. D. Callander, I. Chouroulinkov

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Abstract

1-Chloromethylpyrene (1-CMP) has been evaluated as a model mutagen and toxin related to the ultimate electrophiles derived from benzo[a]pyrene and 1-nitropyrene. It was mutagenic to Salmonella (> 100 pg/plate) an exceptionally reactive to DNA when assessed by the 32P-postlabelling technique. 1-CMP was inactive in a mouse bone micronucleus assay when administered by gavage, probably due to hydrolysis, whose kinetics have been studied (t 1 2 ∼ 23 min at 37°C). However, as expected, it was a potent skin toxin as determined by its activity as a mitogen to mouse skin and its contact allergenicity, as determined using the local lymph node proliferative assay. It is concluded that 1-CMP will probably be a potent human skin carcinogen and contact allergen. © 1990.

Original language	English
Pages (from-to)	281-289
Number of pages	8
Journal	Mutation Research Letters
Volume	243
Issue number	4
Publication status	Published - Apr 1990

Keywords

Bone-marrow micronuclei
DNA adducts
I-Chloromethylpyrene
Skin sensitization

Cite this

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title = "1-Chloromethylpyrene: a reference skin sensitizer and genotoxin",

abstract = "1-Chloromethylpyrene (1-CMP) has been evaluated as a model mutagen and toxin related to the ultimate electrophiles derived from benzo[a]pyrene and 1-nitropyrene. It was mutagenic to Salmonella (> 100 pg/plate) an exceptionally reactive to DNA when assessed by the 32P-postlabelling technique. 1-CMP was inactive in a mouse bone micronucleus assay when administered by gavage, probably due to hydrolysis, whose kinetics have been studied (t 1 2 ∼ 23 min at 37°C). However, as expected, it was a potent skin toxin as determined by its activity as a mitogen to mouse skin and its contact allergenicity, as determined using the local lymph node proliferative assay. It is concluded that 1-CMP will probably be a potent human skin carcinogen and contact allergen. {\textcopyright} 1990.",

keywords = "Bone-marrow micronuclei, DNA adducts, I-Chloromethylpyrene, Skin sensitization",

author = "J. Ashby and Gallagher, {J. E.} and M. Kohan and H. Tinwell and I. Kimber and D. Paton and Callander, {R. D.} and I. Chouroulinkov",

year = "1990",

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volume = "243",

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TY - JOUR

T1 - 1-Chloromethylpyrene: a reference skin sensitizer and genotoxin

AU - Ashby, J.

AU - Gallagher, J. E.

AU - Kohan, M.

AU - Tinwell, H.

AU - Kimber, I.

AU - Paton, D.

AU - Callander, R. D.

AU - Chouroulinkov, I.

PY - 1990/4

Y1 - 1990/4

N2 - 1-Chloromethylpyrene (1-CMP) has been evaluated as a model mutagen and toxin related to the ultimate electrophiles derived from benzo[a]pyrene and 1-nitropyrene. It was mutagenic to Salmonella (> 100 pg/plate) an exceptionally reactive to DNA when assessed by the 32P-postlabelling technique. 1-CMP was inactive in a mouse bone micronucleus assay when administered by gavage, probably due to hydrolysis, whose kinetics have been studied (t 1 2 ∼ 23 min at 37°C). However, as expected, it was a potent skin toxin as determined by its activity as a mitogen to mouse skin and its contact allergenicity, as determined using the local lymph node proliferative assay. It is concluded that 1-CMP will probably be a potent human skin carcinogen and contact allergen. © 1990.

AB - 1-Chloromethylpyrene (1-CMP) has been evaluated as a model mutagen and toxin related to the ultimate electrophiles derived from benzo[a]pyrene and 1-nitropyrene. It was mutagenic to Salmonella (> 100 pg/plate) an exceptionally reactive to DNA when assessed by the 32P-postlabelling technique. 1-CMP was inactive in a mouse bone micronucleus assay when administered by gavage, probably due to hydrolysis, whose kinetics have been studied (t 1 2 ∼ 23 min at 37°C). However, as expected, it was a potent skin toxin as determined by its activity as a mitogen to mouse skin and its contact allergenicity, as determined using the local lymph node proliferative assay. It is concluded that 1-CMP will probably be a potent human skin carcinogen and contact allergen. © 1990.

KW - Bone-marrow micronuclei

KW - DNA adducts

KW - I-Chloromethylpyrene

KW - Skin sensitization

M3 - Article

C2 - 2325693

SN - 0165-7992

VL - 243

SP - 281

EP - 289

JO - Mutation Research Letters

JF - Mutation Research Letters

IS - 4

ER -

1-Chloromethylpyrene: a reference skin sensitizer and genotoxin

Abstract

Keywords

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