1217-P: The Effects of Bariatric Surgery on the Proteome in People Achieving Remission of Type 2 Diabetes (T2DM) after Bariatric Surgery

Bariatric surgery (BS) results in metabolic pathway recallibration. Proteins are important effectors of cellular mechanisms. We identified changes within the proteome following BS in people achieving T2DM remission. SWATH-MS proteomics was carried out on serum samples from 10 people who achieved remission of T2DM post Roux-en-Y gastric bypass or Sleeve gastrectomy at 3 time points: 4/12 prior to surgery + 6/12 and 12/12 follow-up. 25 proteins were differentially expressed between pre-surgery (Figure) and 6/12 post-surgery; 39 proteins between baseline and 12/12. Principal component analysis revealed good resolution between time points when proteins with significant fold changes were included. Only 8 proteins were significantly different to pre-surgery samples at both 6/12 and 12/12 post-surgery. These were: SHBG/Serotransferrin/Proteoglycan4/ApoA-IV, LRG1/HSP70/EPXH2/PGLRYP2. The panel of proteins identified as consistently different, included peptides related to insulin sensitivity (SHBG: log fold chain (LFC) change +1.95), systemic inflammation (serotransferrin: LFC -0.78 and Hsp70: LFC -0.39) and lipid metabolism (ApoA-IV: LFC -1.38). Using the SWATH-MS technique we found significant consistent change in the proteome for 8 proteins from pre-BS to 6/12 and 12/12 post-BS. Several of these are key components in metabolic and inflammatory pathways.