3-D stimulated emission depletion microscopy with programmable aberration correction

Martin O. Lenz, Hugo G. Sinclair, Alexander Savell, James H. Clegg, Alice C N Brown, Daniel M. Davis, Chris Dunsby, Mark A A Neil, Paul M W French

    Research output: Contribution to journalArticlepeer-review


    We present a stimulated emission depletion (STED) microscope that provides 3-D super resolution by simultaneous depletion using beams with both a helical phase profile for enhanced lateral resolution and an annular phase profile to enhance axial resolution. The 3-D depletion point spread function is realised using a single spatial light modulator that can also be programmed to compensate for aberrations in the microscope and the sample. We apply it to demonstrate the first 3-D super-resolved imaging of an immunological synapse between a Natural Killer cell and its target cell. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
    Original languageEnglish
    JournalJournal of biophotonics
    Publication statusPublished - 2013


    • Confocal microscopy
    • Fluorescence microscopy
    • Immune synapse
    • Nanoscopy
    • STED microscopy
    • Super-resolution


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