3-D stimulated emission depletion microscopy with programmable aberration correction

Martin O. Lenz; Hugo G. Sinclair; Alexander Savell; James H. Clegg; Alice C N Brown; Daniel M. Davis; Chris Dunsby; Mark A A Neil; Paul M W French

doi:10.1002/jbio.201300041

3-D stimulated emission depletion microscopy with programmable aberration correction

Martin O. Lenz, Hugo G. Sinclair, Alexander Savell, James H. Clegg, Alice C N Brown, Daniel M. Davis, Chris Dunsby, Mark A A Neil, Paul M W French

Research output: Contribution to journal › Article › peer-review

Abstract

We present a stimulated emission depletion (STED) microscope that provides 3-D super resolution by simultaneous depletion using beams with both a helical phase profile for enhanced lateral resolution and an annular phase profile to enhance axial resolution. The 3-D depletion point spread function is realised using a single spatial light modulator that can also be programmed to compensate for aberrations in the microscope and the sample. We apply it to demonstrate the first 3-D super-resolved imaging of an immunological synapse between a Natural Killer cell and its target cell. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Original language	English
Journal	Journal of biophotonics
DOIs	https://doi.org/10.1002/jbio.201300041
Publication status	Published - 2013

Keywords

Confocal microscopy
Fluorescence microscopy
Immune synapse
Nanoscopy
STED microscopy
Super-resolution

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10.1002/jbio.201300041

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title = "3-D stimulated emission depletion microscopy with programmable aberration correction",

abstract = "We present a stimulated emission depletion (STED) microscope that provides 3-D super resolution by simultaneous depletion using beams with both a helical phase profile for enhanced lateral resolution and an annular phase profile to enhance axial resolution. The 3-D depletion point spread function is realised using a single spatial light modulator that can also be programmed to compensate for aberrations in the microscope and the sample. We apply it to demonstrate the first 3-D super-resolved imaging of an immunological synapse between a Natural Killer cell and its target cell. {\textcopyright} 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",

keywords = "Confocal microscopy, Fluorescence microscopy, Immune synapse, Nanoscopy, STED microscopy, Super-resolution",

author = "Lenz, {Martin O.} and Sinclair, {Hugo G.} and Alexander Savell and Clegg, {James H.} and Brown, {Alice C N} and Davis, {Daniel M.} and Chris Dunsby and Neil, {Mark A A} and French, {Paul M W}",

year = "2013",

doi = "10.1002/jbio.201300041",

language = "English",

journal = "Journal of biophotonics",

issn = "1864-063X",

publisher = "John Wiley & Sons Ltd",

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TY - JOUR

T1 - 3-D stimulated emission depletion microscopy with programmable aberration correction

AU - Lenz, Martin O.

AU - Sinclair, Hugo G.

AU - Savell, Alexander

AU - Clegg, James H.

AU - Brown, Alice C N

AU - Davis, Daniel M.

AU - Dunsby, Chris

AU - Neil, Mark A A

AU - French, Paul M W

PY - 2013

Y1 - 2013

N2 - We present a stimulated emission depletion (STED) microscope that provides 3-D super resolution by simultaneous depletion using beams with both a helical phase profile for enhanced lateral resolution and an annular phase profile to enhance axial resolution. The 3-D depletion point spread function is realised using a single spatial light modulator that can also be programmed to compensate for aberrations in the microscope and the sample. We apply it to demonstrate the first 3-D super-resolved imaging of an immunological synapse between a Natural Killer cell and its target cell. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

AB - We present a stimulated emission depletion (STED) microscope that provides 3-D super resolution by simultaneous depletion using beams with both a helical phase profile for enhanced lateral resolution and an annular phase profile to enhance axial resolution. The 3-D depletion point spread function is realised using a single spatial light modulator that can also be programmed to compensate for aberrations in the microscope and the sample. We apply it to demonstrate the first 3-D super-resolved imaging of an immunological synapse between a Natural Killer cell and its target cell. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

KW - Confocal microscopy

KW - Fluorescence microscopy

KW - Immune synapse

KW - Nanoscopy

KW - STED microscopy

KW - Super-resolution

U2 - 10.1002/jbio.201300041

DO - 10.1002/jbio.201300041

M3 - Article

C2 - 23788459

SN - 1864-063X

JO - Journal of biophotonics

JF - Journal of biophotonics

ER -

3-D stimulated emission depletion microscopy with programmable aberration correction

Abstract

Keywords

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