3D cell bioprinting of self-assembling peptide-based hydrogels

Bella Raphael; Tony Khalil; Victoria Louise Workman; Andrew Smith; Cameron P. Brown; Charles Streuli; Alberto Saiani; Marco Domingos

doi:10.1016/j.matlet.2016.12.127

3D cell bioprinting of self-assembling peptide-based hydrogels

Bella Raphael, Tony Khalil, Victoria Louise Workman, Andrew Smith, Cameron P. Brown, Charles Streuli, Alberto Saiani, Marco Domingos

Division of Cell Matrix Biology & Regenerative Medicine (L5)

Research output: Contribution to journal › Article › peer-review

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Abstract

Bioprinting of 3D cell-laden constructs with well-defined architectures and controlled spatial distribution of cells is gaining importance in the field of Tissue Engineering. New 3D tissue models are being developed to study the complex cellular interactions that take place during both tissue development and in the regeneration of damaged and/or diseased tissues. Despite advances in 3D printing technologies, suitable hydrogels or ‘bioinks’ with enhanced printability and cell viability are lacking. Here we report a study on the 3D bioprinting of a novel group of self-assembling peptide-based hydrogels. Our results demonstrate the ability of the system to print well-defined 3D cell laden constructs with variable stiffness and improved structural integrity, whilst providing a cell-friendly extracellular matrix “like” microenvironment. Biological assays reveal that mammary epithelial cells remain viable after 7 days of in vitro culture, independent of the hydrogel stiffness.

Original language	English
Pages (from-to)	103-106
Journal	Materials Letters
Volume	190
Early online date	31 Dec 2016
DOIs	https://doi.org/10.1016/j.matlet.2016.12.127
Publication status	Published - 31 Dec 2016

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title = "3D cell bioprinting of self-assembling peptide-based hydrogels",

abstract = "Bioprinting of 3D cell-laden constructs with well-defined architectures and controlled spatial distribution of cells is gaining importance in the field of Tissue Engineering. New 3D tissue models are being developed to study the complex cellular interactions that take place during both tissue development and in the regeneration of damaged and/or diseased tissues. Despite advances in 3D printing technologies, suitable hydrogels or {\textquoteleft}bioinks{\textquoteright} with enhanced printability and cell viability are lacking. Here we report a study on the 3D bioprinting of a novel group of self-assembling peptide-based hydrogels. Our results demonstrate the ability of the system to print well-defined 3D cell laden constructs with variable stiffness and improved structural integrity, whilst providing a cell-friendly extracellular matrix “like” microenvironment. Biological assays reveal that mammary epithelial cells remain viable after 7 days of in vitro culture, independent of the hydrogel stiffness.",

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AU - Raphael, Bella

AU - Khalil, Tony

AU - Workman, Victoria Louise

AU - Smith, Andrew

AU - Brown, Cameron P.

AU - Streuli, Charles

AU - Saiani, Alberto

AU - Domingos, Marco

PY - 2016/12/31

Y1 - 2016/12/31

N2 - Bioprinting of 3D cell-laden constructs with well-defined architectures and controlled spatial distribution of cells is gaining importance in the field of Tissue Engineering. New 3D tissue models are being developed to study the complex cellular interactions that take place during both tissue development and in the regeneration of damaged and/or diseased tissues. Despite advances in 3D printing technologies, suitable hydrogels or ‘bioinks’ with enhanced printability and cell viability are lacking. Here we report a study on the 3D bioprinting of a novel group of self-assembling peptide-based hydrogels. Our results demonstrate the ability of the system to print well-defined 3D cell laden constructs with variable stiffness and improved structural integrity, whilst providing a cell-friendly extracellular matrix “like” microenvironment. Biological assays reveal that mammary epithelial cells remain viable after 7 days of in vitro culture, independent of the hydrogel stiffness.

AB - Bioprinting of 3D cell-laden constructs with well-defined architectures and controlled spatial distribution of cells is gaining importance in the field of Tissue Engineering. New 3D tissue models are being developed to study the complex cellular interactions that take place during both tissue development and in the regeneration of damaged and/or diseased tissues. Despite advances in 3D printing technologies, suitable hydrogels or ‘bioinks’ with enhanced printability and cell viability are lacking. Here we report a study on the 3D bioprinting of a novel group of self-assembling peptide-based hydrogels. Our results demonstrate the ability of the system to print well-defined 3D cell laden constructs with variable stiffness and improved structural integrity, whilst providing a cell-friendly extracellular matrix “like” microenvironment. Biological assays reveal that mammary epithelial cells remain viable after 7 days of in vitro culture, independent of the hydrogel stiffness.

U2 - 10.1016/j.matlet.2016.12.127

DO - 10.1016/j.matlet.2016.12.127

M3 - Article

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JO - Materials Letters

JF - Materials Letters

ER -

3D cell bioprinting of self-assembling peptide-based hydrogels

Abstract

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