3D X-Ray Nanotomography of Cells Grown on Electrospun Scaffolds

Robert S. Bradley, Ian K. Robinson, Mohammed Yusuf

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Here, it is demonstrated that X-ray nanotomography with Zernike phase contrast can be used for 3D imaging of cells grown on electrospun polymer scaffolds. The scaffold fibers and cells are simultaneously imaged, enabling the influence of scaffold architecture on cell location and morphology to be studied. The high resolution enables subcellular details to be revealed. The X-ray imaging conditions were optimized to reduce scan times, making it feasible to scan multiple regions of interest in relatively large samples. An image processing procedure is presented which enables scaffold characteristics and cell location to be quantified. The procedure is demonstrated by comparing the ingrowth of cells after culture for 3 and 6 days. (Figure presented.).

    Original languageEnglish
    Article number1600236
    JournalMacromolecular Bioscience
    Volume17
    Issue number2
    Early online date16 Sept 2016
    DOIs
    Publication statusPublished - 15 Feb 2017

    Keywords

    • cell infiltration
    • electrospun scaffolds
    • imaging
    • microstructure
    • X-ray nanotomography

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