53 Effects Of GLP-1 eluting stem cell therapy on collagen remodelling in a porcine model of myocardial infarction.

E J Wright; K A Farrell; N Malik; C Wallrapp; P Geigle; A L Lewis; P W Stratford; C M Holt

doi:10.1136/heartjnl-2011-300920b.53

53 Effects Of GLP-1 eluting stem cell therapy on collagen remodelling in a porcine model of myocardial infarction.

E J Wright, K A Farrell, N Malik, C Wallrapp, P Geigle, A L Lewis, P W Stratford, C M Holt

Research output: Contribution to journal › Article › peer-review

Abstract

Glucagon-like peptide-1 (GLP-1) is an incretin hormone with cardioprotective effects. Human stem cells (hMSCs) secreting a GLP-1 fusion protein and encapsulated in alginate (GLP-1 CellBeads) have been developed as a novel therapeutic. This study investigated the effects of GLP-1 CellBeads on post-MI healing in a porcine model. GLP-1 CellBeads were delivered to coronary artery branches, creating micro-infarcts and mild LV dysfunction. Cell-free beads (empty) and CellBeads containing hMSCs but not secreting GLP-1 (hMSC-beads) were delivered as controls. Left ventricular (LV) function, infarct size, myocyte cross-sectional area, total collagen content (picrosirius red staining) and collagen -1 and -3 levels (qRT-PCR) were analysed to determine therapeutic potential. Four weeks post-MI, only GLP-1 CellBead treatment produced significantly improved LV function compared to 30 min post-MI (44.00%±1.29% vs 49.75%±1.03%, p

Original language	English
Journal	Heart (British Cardiac Society)
Volume	97
Issue number	20
DOIs	https://doi.org/10.1136/heartjnl-2011-300920b.53
Publication status	Published - Oct 2011

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10.1136/heartjnl-2011-300920b.53

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title = "53 Effects Of GLP-1 eluting stem cell therapy on collagen remodelling in a porcine model of myocardial infarction.",

abstract = "Glucagon-like peptide-1 (GLP-1) is an incretin hormone with cardioprotective effects. Human stem cells (hMSCs) secreting a GLP-1 fusion protein and encapsulated in alginate (GLP-1 CellBeads) have been developed as a novel therapeutic. This study investigated the effects of GLP-1 CellBeads on post-MI healing in a porcine model. GLP-1 CellBeads were delivered to coronary artery branches, creating micro-infarcts and mild LV dysfunction. Cell-free beads (empty) and CellBeads containing hMSCs but not secreting GLP-1 (hMSC-beads) were delivered as controls. Left ventricular (LV) function, infarct size, myocyte cross-sectional area, total collagen content (picrosirius red staining) and collagen -1 and -3 levels (qRT-PCR) were analysed to determine therapeutic potential. Four weeks post-MI, only GLP-1 CellBead treatment produced significantly improved LV function compared to 30 min post-MI (44.00%±1.29% vs 49.75%±1.03%, p",

author = "Wright, {E J} and Farrell, {K A} and N Malik and C Wallrapp and P Geigle and Lewis, {A L} and Stratford, {P W} and Holt, {C M}",

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AU - Wright, E J

AU - Farrell, K A

AU - Malik, N

AU - Wallrapp, C

AU - Geigle, P

AU - Lewis, A L

AU - Stratford, P W

AU - Holt, C M

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N2 - Glucagon-like peptide-1 (GLP-1) is an incretin hormone with cardioprotective effects. Human stem cells (hMSCs) secreting a GLP-1 fusion protein and encapsulated in alginate (GLP-1 CellBeads) have been developed as a novel therapeutic. This study investigated the effects of GLP-1 CellBeads on post-MI healing in a porcine model. GLP-1 CellBeads were delivered to coronary artery branches, creating micro-infarcts and mild LV dysfunction. Cell-free beads (empty) and CellBeads containing hMSCs but not secreting GLP-1 (hMSC-beads) were delivered as controls. Left ventricular (LV) function, infarct size, myocyte cross-sectional area, total collagen content (picrosirius red staining) and collagen -1 and -3 levels (qRT-PCR) were analysed to determine therapeutic potential. Four weeks post-MI, only GLP-1 CellBead treatment produced significantly improved LV function compared to 30 min post-MI (44.00%±1.29% vs 49.75%±1.03%, p

AB - Glucagon-like peptide-1 (GLP-1) is an incretin hormone with cardioprotective effects. Human stem cells (hMSCs) secreting a GLP-1 fusion protein and encapsulated in alginate (GLP-1 CellBeads) have been developed as a novel therapeutic. This study investigated the effects of GLP-1 CellBeads on post-MI healing in a porcine model. GLP-1 CellBeads were delivered to coronary artery branches, creating micro-infarcts and mild LV dysfunction. Cell-free beads (empty) and CellBeads containing hMSCs but not secreting GLP-1 (hMSC-beads) were delivered as controls. Left ventricular (LV) function, infarct size, myocyte cross-sectional area, total collagen content (picrosirius red staining) and collagen -1 and -3 levels (qRT-PCR) were analysed to determine therapeutic potential. Four weeks post-MI, only GLP-1 CellBead treatment produced significantly improved LV function compared to 30 min post-MI (44.00%±1.29% vs 49.75%±1.03%, p

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DO - 10.1136/heartjnl-2011-300920b.53

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JO - Heart (British Cardiac Society)

JF - Heart (British Cardiac Society)

IS - 20

ER -

53 Effects Of GLP-1 eluting stem cell therapy on collagen remodelling in a porcine model of myocardial infarction.

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