A biocatalytic cascade for the amination of unfunctionalised cycloalkanes

Michele Tavanti, Juan Mangas-Sanchez, Sarah L. Montgomery, Matthew P. Thompson, Nicholas J. Turner

    Research output: Contribution to journalArticlepeer-review

    185 Downloads (Pure)


    Here we describe a one-pot, three-enzyme, cascade involving a cytochrome P450 monooxygenase, an alcohol dehydrogenase and a reductive aminase for the synthesis of secondary amines from cycloalkanes. Amine product concentrations of up to 19.6 mM were achieved. The preparative scale amination of cyclohexane was also demonstrated with a space-time yield of 2 g L-1 d-1.

    Original languageEnglish
    Pages (from-to)9790-9793
    Number of pages4
    JournalOrganic and Biomolecular Chemistry
    Issue number46
    Early online date10 Nov 2017
    Publication statusPublished - 14 Dec 2017

    Research Beacons, Institutes and Platforms

    • Manchester Institute of Biotechnology


    Dive into the research topics of 'A biocatalytic cascade for the amination of unfunctionalised cycloalkanes'. Together they form a unique fingerprint.

    Cite this