A biocatalytic cascade for the amination of unfunctionalised cycloalkanes

Michele Tavanti; Juan Mangas-Sanchez; Sarah L. Montgomery; Matthew P. Thompson; Nicholas J. Turner

doi:10.1039/c7ob02569f

A biocatalytic cascade for the amination of unfunctionalised cycloalkanes

Michele Tavanti, Juan Mangas-Sanchez, Sarah L. Montgomery, Matthew P. Thompson, Nicholas J. Turner^*

^*Corresponding author for this work

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Abstract

Here we describe a one-pot, three-enzyme, cascade involving a cytochrome P450 monooxygenase, an alcohol dehydrogenase and a reductive aminase for the synthesis of secondary amines from cycloalkanes. Amine product concentrations of up to 19.6 mM were achieved. The preparative scale amination of cyclohexane was also demonstrated with a space-time yield of 2 g L^-1 d^-1.

Original language	English
Pages (from-to)	9790-9793
Number of pages	4
Journal	Organic and Biomolecular Chemistry
Volume	15
Issue number	46
Early online date	10 Nov 2017
DOIs	https://doi.org/10.1039/c7ob02569f
Publication status	Published - 14 Dec 2017

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Manchester Institute of Biotechnology

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abstract = "Here we describe a one-pot, three-enzyme, cascade involving a cytochrome P450 monooxygenase, an alcohol dehydrogenase and a reductive aminase for the synthesis of secondary amines from cycloalkanes. Amine product concentrations of up to 19.6 mM were achieved. The preparative scale amination of cyclohexane was also demonstrated with a space-time yield of 2 g L-1 d-1.",

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AU - Tavanti, Michele

AU - Mangas-Sanchez, Juan

AU - Montgomery, Sarah L.

AU - Thompson, Matthew P.

AU - Turner, Nicholas J.

PY - 2017/12/14

Y1 - 2017/12/14

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AB - Here we describe a one-pot, three-enzyme, cascade involving a cytochrome P450 monooxygenase, an alcohol dehydrogenase and a reductive aminase for the synthesis of secondary amines from cycloalkanes. Amine product concentrations of up to 19.6 mM were achieved. The preparative scale amination of cyclohexane was also demonstrated with a space-time yield of 2 g L-1 d-1.

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JO - Organic and Biomolecular Chemistry

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ER -

A biocatalytic cascade for the amination of unfunctionalised cycloalkanes

Abstract

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