A bromodeoxyuridine (BrdU) based protocol for characterizing proliferating progenitors in Xenopus embryos.

Hélène Auger; Raphaël Thuret; Warif El Yakoubi; Nancy Papalopulu

doi:10.1007/978-1-61779-992-1_26

A bromodeoxyuridine (BrdU) based protocol for characterizing proliferating progenitors in Xenopus embryos.

Hélène Auger, Raphaël Thuret, Warif El Yakoubi, Nancy Papalopulu

Research output: Contribution to journal › Article › peer-review

Abstract

BrdU is a thymidine analog that is incorporated into DNA during the S-phase of the cell cycle. BrdU incorporation can be used to quantify the number of cells that are in S-phase in the time period that BrdU is available. Thus, BrdU incorporation is an essential method in the quantitative analysis of cell proliferation, during normal embryonic development or after experimental manipulation. It is a reliable and versatile method that can be easily combined with immunohistochemistry and in situ hybridization to relate cell proliferation with gene expression. BrdU incorporation has been used in all model organisms; here, we describe a protocol adapted for use in Xenopus embryos.

Original language	English
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	917
DOIs	https://doi.org/10.1007/978-1-61779-992-1_26
Publication status	Published - 2012

Access to Document

10.1007/978-1-61779-992-1_26

Cite this

@article{545cf7ad083e46dd9e7fc44dc7d38fd9,

title = "A bromodeoxyuridine (BrdU) based protocol for characterizing proliferating progenitors in Xenopus embryos.",

abstract = "BrdU is a thymidine analog that is incorporated into DNA during the S-phase of the cell cycle. BrdU incorporation can be used to quantify the number of cells that are in S-phase in the time period that BrdU is available. Thus, BrdU incorporation is an essential method in the quantitative analysis of cell proliferation, during normal embryonic development or after experimental manipulation. It is a reliable and versatile method that can be easily combined with immunohistochemistry and in situ hybridization to relate cell proliferation with gene expression. BrdU incorporation has been used in all model organisms; here, we describe a protocol adapted for use in Xenopus embryos.",

author = "H{\'e}l{\`e}ne Auger and Rapha{\"e}l Thuret and {El Yakoubi}, Warif and Nancy Papalopulu",

note = "090868, Wellcome Trust, United Kingdom, Wellcome Trust, United Kingdom",

year = "2012",

doi = "10.1007/978-1-61779-992-1_26",

language = "English",

volume = "917",

journal = "Methods in molecular biology (Clifton, N.J.)",

issn = "1064-3745",

publisher = "Humana Press Inc.",

}

TY - JOUR

T1 - A bromodeoxyuridine (BrdU) based protocol for characterizing proliferating progenitors in Xenopus embryos.

AU - Auger, Hélène

AU - Thuret, Raphaël

AU - El Yakoubi, Warif

AU - Papalopulu, Nancy

N1 - 090868, Wellcome Trust, United Kingdom, Wellcome Trust, United Kingdom

PY - 2012

Y1 - 2012

N2 - BrdU is a thymidine analog that is incorporated into DNA during the S-phase of the cell cycle. BrdU incorporation can be used to quantify the number of cells that are in S-phase in the time period that BrdU is available. Thus, BrdU incorporation is an essential method in the quantitative analysis of cell proliferation, during normal embryonic development or after experimental manipulation. It is a reliable and versatile method that can be easily combined with immunohistochemistry and in situ hybridization to relate cell proliferation with gene expression. BrdU incorporation has been used in all model organisms; here, we describe a protocol adapted for use in Xenopus embryos.

AB - BrdU is a thymidine analog that is incorporated into DNA during the S-phase of the cell cycle. BrdU incorporation can be used to quantify the number of cells that are in S-phase in the time period that BrdU is available. Thus, BrdU incorporation is an essential method in the quantitative analysis of cell proliferation, during normal embryonic development or after experimental manipulation. It is a reliable and versatile method that can be easily combined with immunohistochemistry and in situ hybridization to relate cell proliferation with gene expression. BrdU incorporation has been used in all model organisms; here, we describe a protocol adapted for use in Xenopus embryos.

U2 - 10.1007/978-1-61779-992-1_26

DO - 10.1007/978-1-61779-992-1_26

M3 - Article

C2 - 22956104

SN - 1064-3745

VL - 917

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

A bromodeoxyuridine (BrdU) based protocol for characterizing proliferating progenitors in Xenopus embryos.

Abstract

Access to Document

Fingerprint

Cite this