A Chemical Genomic Approach Identifies Matrix Metalloproteinases as Playing an Essential and Specific Role in Xenopus Melanophore Migration

M.L. Tomlinson, P. Guan, R.J. Morris, M.D. Fidock, M. Rejzek, C. Garcia-Morales, R.A. Field, G.N. Wheeler

Research output: Contribution to journalArticlepeer-review


To dissect the function of matrix metalloproteinases (MMPs) involved in cellular migration in vivo, we undertook both a forward chemical genomic screen and a functional approach to discover modulators of melanophore (pigment cell) migration in Xenopus laevis. We identified the 8-quinolinol derivative NSC 84093 as affecting melanophore migration in the developing embryo and have shown it to act as a MMP inhibitor. Potential targets of NSC 84093 investigated include MMP-14 and MMP-2. MMP-14 is expressed in migrating neural crest cells from which melanophores are derived. MMP-2 is expressed at the relevant time of development and in a pattern that suggests it contributes to melanophore migration. Morpholino-mediated knockdown of both MMPs demonstrates they play a key role in melanophore migration and partially phenocopy the effect of NSC 84093. © 2009 Elsevier Ltd. All rights reserved.
Original languageUndefined
Pages (from-to)93-104
Number of pages12
JournalChemistry and Biology
Publication statusPublished - 2009


  • Matrix Metalloproteinase 14
  • Matrix Metalloproteinases
  • Metalloproteinase MT1-MMP

Research Beacons, Institutes and Platforms

  • Manchester Institute of Biotechnology

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