A common chemical mechanism used for DNA cleavage by copper(II) activated by thiols and ascorbate is distinct from that for copper(II): Hydrogen peroxide cleavage

David C A John, Kenneth T. Douglas

    Research output: Contribution to journalArticlepeer-review

    Abstract

    By use of a [32P] end-labelled 163 base pair linear fragment of duplex DNA isolated from tyrTDNA, the apparent sequence selectivity previously reported for DNA cleavage by CuII:thiol systems was shown to be identical to the sequence selectivity in scission shown by the CuII:ascorbate system. On this basis, a general mechanism of DNA cleavage is proposed for CuII with reducing agents including thiols as well as reducing sugars, including ascorbate and glucosamine. This mechanism involves hydroxyl radical generation and leads to both base damage and strand scission, and based on results with CuII:thiol cleavage the former occurs before the latter is detectable. Cleavage of DNA by CuII:hydrogen peroxide follows a different chemical mechanism, distinguishable by its different sequence preference. © 1996 Chapman & Hall.
    Original languageEnglish
    Pages (from-to)460-463
    Number of pages3
    JournalTransition Metal Chemistry
    Volume21
    Issue number5
    Publication statusPublished - Oct 1996

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