A detailed mammosphere assay protocol for the quantification of breast stem cell activity

Frances L. Shaw, Hannah Harrison, Katherine Spence, Matthew P. Ablett, Bruno M. Simões, Gillian Farnie, Robert B. Clarke

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Since the discovery that neural tissue contains a population of stem cells that form neurospheres in vitro, sphere-forming assays have been adapted for use with a number of different tissue types for the quantification of stem cell activity and self-renewal. One tissue type widely used for stem cell investigations is mammary tissue, and the mammosphere assay has been used in both normal tissue and cancer. Although it is a relatively simple assay to learn, it can be difficult to master. There are methodological and analytical aspects to the assay which require careful consideration when interpreting the results. We describe here a detailed mammosphere assay protocol for the assessment of stem cell activity and self-renewal, and discuss how data generated by the assay can be analysed and interpreted. © Springer Science+Business Media, LLC 2012.
    Original languageEnglish
    Pages (from-to)111-117
    Number of pages6
    JournalJournal of Mammary Gland Biology and Neoplasia
    Volume17
    Issue number2
    DOIs
    Publication statusPublished - Jun 2012

    Keywords

    • Breast
    • Cancer
    • Mammosphere assay
    • Stemcell

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