A drosophila in vivo screen identifies store-operated calcium entry as a key regulator of adiposity

Jens Baumbach, Petra Hummel, Iris Bickmeyer, Katarzyna M. Kowalczyk, Martina Frank, Konstantin Knorr, Anja Hildebrandt, Dietmar Riedel, Herbert Jäckle, Ronald P. Kühnlein

    Research output: Contribution to journalArticlepeer-review

    Abstract

    To unravel the evolutionarily conserved genetic network underlying energy homeostasis, we performed a systematic in vivo gene knockdown screen in Drosophila. We used a transgenic RNAi library enriched for fly orthologs of human genes to functionally impair about half of all Drosophila genes specifically in adult fat storage tissue. This approach identified 77 genes, which affect the body fat content of the fly, including 58 previously unknown obesity-associated genes. These genes function in diverse biological processes such as lipid metabolism, vesicle-mediated trafficking, and the universal store-operated calcium entry (SOCE). Impairment of the SOCE core component Stromal interaction molecule (Stim), as well as other components of the pathway, causes adiposity in flies. Acute Stim dysfunction in the fat storage tissue triggers hyperphagia via remote control of the orexigenic short neuropeptide F in the brain, which in turn affects the coordinated lipogenic and lipolytic gene regulation, resulting in adipose tissue hypertrophy.

    Original languageEnglish
    Pages (from-to)331-343
    Number of pages13
    JournalCell Metabolism
    Volume19
    Issue number2
    DOIs
    Publication statusPublished - 4 Feb 2014

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