A fluorometric assay of the degree of modification of protein primary amines with polyethylene glycol

S. Jill Stocks, A. J M Jones, Charles W. Ramey, Donald E. Brooks

    Research output: Contribution to journalArticlepeer-review

    Abstract

    An alternative primary amine assay utilizing 4-phenylspiro[furan-2(3H), 1′-phthalan]-3,3′-dione (fluorescamine) is compared with the frequently applied trinitrobenzenesulfonic acid assay as a means of estimating protein primary amine modification with cyanuric chloride attachment of polyethylene glycol (PEG). The results of the two assays are compared for three different proteins and several advantages of the fluorescamine assay are emphasized. In particular, the fluorescamine assay was found to be unaffected by free PEG up to 0.08% in the assay mixture. The fluorescamine assay can be used to measure the partition coefficients of native or PEG-modified protein in two-phase aqueous polymer systems. © 1986.
    Original languageEnglish
    Pages (from-to)232-234
    Number of pages2
    JournalAnalytical Biochemistry
    Volume154
    Issue number1
    Publication statusPublished - Apr 1986

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