A fluorometric assay of the degree of modification of protein primary amines with polyethylene glycol

S. Jill Stocks; A. J M Jones; Charles W. Ramey; Donald E. Brooks

A fluorometric assay of the degree of modification of protein primary amines with polyethylene glycol

S. Jill Stocks, A. J M Jones, Charles W. Ramey, Donald E. Brooks

Research output: Contribution to journal › Article › peer-review

Abstract

An alternative primary amine assay utilizing 4-phenylspiro[furan-2(3H), 1′-phthalan]-3,3′-dione (fluorescamine) is compared with the frequently applied trinitrobenzenesulfonic acid assay as a means of estimating protein primary amine modification with cyanuric chloride attachment of polyethylene glycol (PEG). The results of the two assays are compared for three different proteins and several advantages of the fluorescamine assay are emphasized. In particular, the fluorescamine assay was found to be unaffected by free PEG up to 0.08% in the assay mixture. The fluorescamine assay can be used to measure the partition coefficients of native or PEG-modified protein in two-phase aqueous polymer systems. © 1986.

Original language	English
Pages (from-to)	232-234
Number of pages	2
Journal	Analytical Biochemistry
Volume	154
Issue number	1
Publication status	Published - Apr 1986

Cite this

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title = "A fluorometric assay of the degree of modification of protein primary amines with polyethylene glycol",

abstract = "An alternative primary amine assay utilizing 4-phenylspiro[furan-2(3H), 1′-phthalan]-3,3′-dione (fluorescamine) is compared with the frequently applied trinitrobenzenesulfonic acid assay as a means of estimating protein primary amine modification with cyanuric chloride attachment of polyethylene glycol (PEG). The results of the two assays are compared for three different proteins and several advantages of the fluorescamine assay are emphasized. In particular, the fluorescamine assay was found to be unaffected by free PEG up to 0.08% in the assay mixture. The fluorescamine assay can be used to measure the partition coefficients of native or PEG-modified protein in two-phase aqueous polymer systems. {\textcopyright} 1986.",

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T1 - A fluorometric assay of the degree of modification of protein primary amines with polyethylene glycol

AU - Stocks, S. Jill

AU - Jones, A. J M

AU - Ramey, Charles W.

AU - Brooks, Donald E.

PY - 1986/4

Y1 - 1986/4

N2 - An alternative primary amine assay utilizing 4-phenylspiro[furan-2(3H), 1′-phthalan]-3,3′-dione (fluorescamine) is compared with the frequently applied trinitrobenzenesulfonic acid assay as a means of estimating protein primary amine modification with cyanuric chloride attachment of polyethylene glycol (PEG). The results of the two assays are compared for three different proteins and several advantages of the fluorescamine assay are emphasized. In particular, the fluorescamine assay was found to be unaffected by free PEG up to 0.08% in the assay mixture. The fluorescamine assay can be used to measure the partition coefficients of native or PEG-modified protein in two-phase aqueous polymer systems. © 1986.

AB - An alternative primary amine assay utilizing 4-phenylspiro[furan-2(3H), 1′-phthalan]-3,3′-dione (fluorescamine) is compared with the frequently applied trinitrobenzenesulfonic acid assay as a means of estimating protein primary amine modification with cyanuric chloride attachment of polyethylene glycol (PEG). The results of the two assays are compared for three different proteins and several advantages of the fluorescamine assay are emphasized. In particular, the fluorescamine assay was found to be unaffected by free PEG up to 0.08% in the assay mixture. The fluorescamine assay can be used to measure the partition coefficients of native or PEG-modified protein in two-phase aqueous polymer systems. © 1986.

M3 - Article

C2 - 3706727

SN - 0003-2697

VL - 154

SP - 232

EP - 234

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

A fluorometric assay of the degree of modification of protein primary amines with polyethylene glycol

Abstract

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