A fragment of recombinant GABA(A) receptor alpha 1 subunit forming rosette-like homo-oligomers

H Xue, H Zheng, HM Li, A Kitmitto, HM Zhu, P Lee, A. Holzenburg

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The type A gamma-aminobutyric acid (GABA,) receptor plays a major role in inhibitory synaptic transmission in the central nervous system. A fragment consisting of residues Cys166 to Leu296 of the oil subunit of the GABA(A) receptor was overexpressed in Escherichia coli and was found to have stable beta-rich structures. Here, results from laser scattering, gel electrophoresis and electron microscopy demonstrated that this recombinant protein formed rosette-like homo-oligomers, mainly pentamers in solution. Therefore, the fragment apparently provides a valuable model system for studying the pentameric holoreceptor assembly. Non-reducing sodium dodecyl sulfate polyacrylamide gel electrophoresis of the fragment showed that disulfide bonds formed between monomers contributed to the oligomerization of the fragment. The fact that this fragment alone could form pentamers in vitro strongly suggests that amino acid residues located within the Cys166-Leu296 region of the alpha(1) subunit may contribute to the oligomerization of GABA(A) receptor in vivo. (C) 2000 Academic Press.
    Original languageEnglish
    JournalJournal of molecular biology
    Volume296, 3
    Publication statusPublished - 2000

    Keywords

    • disulfide bond; electron microscopy; homo-oligomeric; laser scattering;
    • non-reducing SDS-PAGE

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