Abstract
Characterizing the protein factors released from placentae during pathogenesis remains a key objective toward understanding preeclampsia and related pregnancy disorders. Gel-free proteomics technologies applied to placental explant-conditioned media offers the potential of identifying these factors. Relative quantification mass spectrometry using isobaric tagging for relative and absolute quantification (iTRAQ) labeling was employed to compare the "secretome" between healthy term placental tissue cultured under both normoxic and hypoxic oxygen tensions. Of the 499 proteins identified, 45 were differentially expressed (P
Original language | English |
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Pages (from-to) | 247-257 |
Number of pages | 10 |
Journal | REPRODUCTIVE SCIENCES |
Volume | 17 |
Issue number | 3 |
DOIs | |
Publication status | Published - Mar 2010 |
Keywords
- Extracellular matrix
- Hypoxia
- IL-8.
- Placenta
- Proteomics