A gel-free quantitative proteomics analysis of factors released from hypoxic-conditioned placentae

Richard T. Blankley, Nicola J. Robinson, John D. Aplin, Ian P. Crocker, Simon J. Gaskell, Anthony D. Whetton, Philip N. Baker, Jenny E. Myers

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Characterizing the protein factors released from placentae during pathogenesis remains a key objective toward understanding preeclampsia and related pregnancy disorders. Gel-free proteomics technologies applied to placental explant-conditioned media offers the potential of identifying these factors. Relative quantification mass spectrometry using isobaric tagging for relative and absolute quantification (iTRAQ) labeling was employed to compare the "secretome" between healthy term placental tissue cultured under both normoxic and hypoxic oxygen tensions. Of the 499 proteins identified, 45 were differentially expressed (P
    Original languageEnglish
    Pages (from-to)247-257
    Number of pages10
    JournalREPRODUCTIVE SCIENCES
    Volume17
    Issue number3
    DOIs
    Publication statusPublished - Mar 2010

    Keywords

    • Extracellular matrix
    • Hypoxia
    • IL-8.
    • Placenta
    • Proteomics

    Fingerprint

    Dive into the research topics of 'A gel-free quantitative proteomics analysis of factors released from hypoxic-conditioned placentae'. Together they form a unique fingerprint.

    Cite this