A general approach to isolating Plasmodium falciparum genes using non-redundant oligonucleotides inferred from protein sequences of other organisms

John E. Hyde; Susan L. Kelly; Stephen P. Holloway; Valerie A. Snewin; Paul F G Sims

doi:10.1016/0166-6851(89)90074-1

A general approach to isolating Plasmodium falciparum genes using non-redundant oligonucleotides inferred from protein sequences of other organisms

John E. Hyde, Susan L. Kelly, Stephen P. Holloway, Valerie A. Snewin, Paul F G Sims

Research output: Contribution to journal › Article › peer-review

Abstract

We have constructed a number of oligonucleotide probes and tested their utility in identifying various genes in Plasmodium falciparum. The probe sequences were based on known conserved regions of proteins from other organisms, coupled with an analysis of the codon usage of the parasite. By using long single oligonucleotides, we have successfully isolated the DHFR-TS gene, two actin genes and two tubulin genes from the K1 (Thailand) isolate of P. falciparum. We compare these single probes to multiply-redundant short oligonucleotide probes and to heterologous probes. We also present a detailed quantitative analysis of optimal probe design, and of how this approach can best be implemented as a general method of isolating plasmodial genes. © 1989.

Original language	English
Pages (from-to)	247-261
Number of pages	14
Journal	Molecular and biochemical parasitology
Volume	32
Issue number	2-3
DOIs	https://doi.org/10.1016/0166-6851(89)90074-1
Publication status	Published - 15 Jan 1989

Keywords

Actin
Codon match index
Dihydrofolate reductase-thymidylate synthetase
Nucleic acid hybridisation
Oligonucleotide probe
Tubulin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0166-6851(89)90074-1

Cite this

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title = "A general approach to isolating Plasmodium falciparum genes using non-redundant oligonucleotides inferred from protein sequences of other organisms",

abstract = "We have constructed a number of oligonucleotide probes and tested their utility in identifying various genes in Plasmodium falciparum. The probe sequences were based on known conserved regions of proteins from other organisms, coupled with an analysis of the codon usage of the parasite. By using long single oligonucleotides, we have successfully isolated the DHFR-TS gene, two actin genes and two tubulin genes from the K1 (Thailand) isolate of P. falciparum. We compare these single probes to multiply-redundant short oligonucleotide probes and to heterologous probes. We also present a detailed quantitative analysis of optimal probe design, and of how this approach can best be implemented as a general method of isolating plasmodial genes. {\textcopyright} 1989.",

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T1 - A general approach to isolating Plasmodium falciparum genes using non-redundant oligonucleotides inferred from protein sequences of other organisms

AU - Hyde, John E.

AU - Kelly, Susan L.

AU - Holloway, Stephen P.

AU - Snewin, Valerie A.

AU - Sims, Paul F G

PY - 1989/1/15

Y1 - 1989/1/15

N2 - We have constructed a number of oligonucleotide probes and tested their utility in identifying various genes in Plasmodium falciparum. The probe sequences were based on known conserved regions of proteins from other organisms, coupled with an analysis of the codon usage of the parasite. By using long single oligonucleotides, we have successfully isolated the DHFR-TS gene, two actin genes and two tubulin genes from the K1 (Thailand) isolate of P. falciparum. We compare these single probes to multiply-redundant short oligonucleotide probes and to heterologous probes. We also present a detailed quantitative analysis of optimal probe design, and of how this approach can best be implemented as a general method of isolating plasmodial genes. © 1989.

AB - We have constructed a number of oligonucleotide probes and tested their utility in identifying various genes in Plasmodium falciparum. The probe sequences were based on known conserved regions of proteins from other organisms, coupled with an analysis of the codon usage of the parasite. By using long single oligonucleotides, we have successfully isolated the DHFR-TS gene, two actin genes and two tubulin genes from the K1 (Thailand) isolate of P. falciparum. We compare these single probes to multiply-redundant short oligonucleotide probes and to heterologous probes. We also present a detailed quantitative analysis of optimal probe design, and of how this approach can best be implemented as a general method of isolating plasmodial genes. © 1989.

KW - Actin

KW - Codon match index

KW - Dihydrofolate reductase-thymidylate synthetase

KW - Nucleic acid hybridisation

KW - Oligonucleotide probe

KW - Tubulin

U2 - 10.1016/0166-6851(89)90074-1

DO - 10.1016/0166-6851(89)90074-1

M3 - Article

C2 - 2648142

SN - 0166-6851

VL - 32

SP - 247

EP - 261

JO - Molecular and biochemical parasitology

JF - Molecular and biochemical parasitology

IS - 2-3

ER -

A general approach to isolating Plasmodium falciparum genes using non-redundant oligonucleotides inferred from protein sequences of other organisms

Abstract

Keywords

UN SDGs

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