A method for linking yeast artificial chromosomes

Zoia Larin; Stephen S. Taylor; Chris Tyler-Smith

doi:10.1093/nar/24.21.4192

A method for linking yeast artificial chromosomes

Zoia Larin, Stephen S. Taylor, Chris Tyler-Smith

Research output: Contribution to journal › Article › peer-review

Abstract

A method for linking any standard yeast artificial chromosomes (YAC) is described. YACs are introduced into the same cell and joined by mitotic recombination between the vector arms and the homologous sequence in a linking vector; several YACs can be recombined sequentially. The linking vectors also contain the β-galactosidase gene as an expression reporter in mammalian cells.

Original language	English
Pages (from-to)	4192-4196
Number of pages	4
Journal	Nucleic acids research.
Volume	24
Issue number	21
DOIs	https://doi.org/10.1093/nar/24.21.4192
Publication status	Published - 1996

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title = "A method for linking yeast artificial chromosomes",

abstract = "A method for linking any standard yeast artificial chromosomes (YAC) is described. YACs are introduced into the same cell and joined by mitotic recombination between the vector arms and the homologous sequence in a linking vector; several YACs can be recombined sequentially. The linking vectors also contain the β-galactosidase gene as an expression reporter in mammalian cells.",

author = "Zoia Larin and Taylor, {Stephen S.} and Chris Tyler-Smith",

year = "1996",

doi = "10.1093/nar/24.21.4192",

language = "English",

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AU - Larin, Zoia

AU - Taylor, Stephen S.

AU - Tyler-Smith, Chris

PY - 1996

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AB - A method for linking any standard yeast artificial chromosomes (YAC) is described. YACs are introduced into the same cell and joined by mitotic recombination between the vector arms and the homologous sequence in a linking vector; several YACs can be recombined sequentially. The linking vectors also contain the β-galactosidase gene as an expression reporter in mammalian cells.

U2 - 10.1093/nar/24.21.4192

DO - 10.1093/nar/24.21.4192

M3 - Article

C2 - 8932371

SN - 1362-4962

VL - 24

SP - 4192

EP - 4196

JO - Nucleic acids research.

JF - Nucleic acids research.

IS - 21

ER -

A method for linking yeast artificial chromosomes

Abstract

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