A method for separation of trehalose from insect hemolymph

Karl J. Kramer; Roy D. Speirs; Cynthia N. Childs

A method for separation of trehalose from insect hemolymph

Karl J. Kramer, Roy D. Speirs, Cynthia N. Childs

Research output: Contribution to journal › Article › peer-review

Abstract

A method was developed for the determination of trehalose levels in insect hemolymph. The disaccharide is first purified by gel-permeation chromatography and then quantitated by the anthrone colorimetric procedure. The concentration of trehalose in hemolymph from eleven species is reported. The method is applicable to determinations in other tissues and organisms as well. © 1978.

Original language	English
Pages (from-to)	692-696
Number of pages	4
Journal	Analytical Biochemistry
Volume	86
Issue number	2
Publication status	Published - 1 Jun 1978

Keywords

Animals
Chromatography, Gel
isolation & purification: Disaccharides
analysis: Hemolymph
analysis: Insects
Larva
Pupa
isolation & purification: Trehalose

Cite this

@article{b343ffca77d346db98fa292ed08a2693,

title = "A method for separation of trehalose from insect hemolymph",

abstract = "A method was developed for the determination of trehalose levels in insect hemolymph. The disaccharide is first purified by gel-permeation chromatography and then quantitated by the anthrone colorimetric procedure. The concentration of trehalose in hemolymph from eleven species is reported. The method is applicable to determinations in other tissues and organisms as well. {\textcopyright} 1978.",

keywords = "Animals, Chromatography, Gel, isolation & purification: Disaccharides, analysis: Hemolymph, analysis: Insects, Larva, Pupa, isolation & purification: Trehalose",

author = "Kramer, {Karl J.} and Speirs, {Roy D.} and Childs, {Cynthia N.}",

year = "1978",

month = jun,

day = "1",

language = "English",

volume = "86",

pages = "692--696",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press, Ltd",

number = "2",

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TY - JOUR

T1 - A method for separation of trehalose from insect hemolymph

AU - Kramer, Karl J.

AU - Speirs, Roy D.

AU - Childs, Cynthia N.

PY - 1978/6/1

Y1 - 1978/6/1

N2 - A method was developed for the determination of trehalose levels in insect hemolymph. The disaccharide is first purified by gel-permeation chromatography and then quantitated by the anthrone colorimetric procedure. The concentration of trehalose in hemolymph from eleven species is reported. The method is applicable to determinations in other tissues and organisms as well. © 1978.

AB - A method was developed for the determination of trehalose levels in insect hemolymph. The disaccharide is first purified by gel-permeation chromatography and then quantitated by the anthrone colorimetric procedure. The concentration of trehalose in hemolymph from eleven species is reported. The method is applicable to determinations in other tissues and organisms as well. © 1978.

KW - Animals

KW - Chromatography, Gel

KW - isolation & purification: Disaccharides

KW - analysis: Hemolymph

KW - analysis: Insects

KW - Larva

KW - Pupa

KW - isolation & purification: Trehalose

M3 - Article

SN - 0003-2697

VL - 86

SP - 692

EP - 696

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

A method for separation of trehalose from insect hemolymph

Abstract

Keywords

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