A modular self-assembly approach to functionalised β-sheet peptide hydrogel biomaterials

P. J. S. King, Maria Giovanna Lizio, Andrew Booth, Richard Collins, Julie Gough, Aline Miller, Simon Webb

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Two complementary β-sheet-forming decapeptides have been created that form binary self-repairing hydrogels upon combination of the respective free-flowing peptide solutions at pH 7 and <0.2 wt %. The component peptides showed little structure separately but formed extended β-sheet fibres upon mixing, which became entangled to produce stiff hydrogels. Microscopy revealed two major structures; thin fibrils with a twisted or helical appearance and with widths comparable to the predicted lengths of the peptides within a β-sheet, and thicker, longer, interwoven fibres that appear to comprise laterally-packed fibrils. A range of gel stiffnesses (G’ from 0.05 to 100 kPa) could be attained in this system by altering the assembly conditions, stiffnesses that covered the rheological properties desirable for cell culture scaffolds. Doping in a RGD-tagged component peptide at 5 mol % improved 3T3 fibroblast attachment and viability compared to hydrogel fibres without RGD functionalisation.
    Original languageEnglish
    Pages (from-to)1915-1923
    Number of pages9
    JournalSoft Matter
    Volume12
    Issue number6
    Early online date18 Dec 2015
    DOIs
    Publication statusPublished - 14 Feb 2016

    Research Beacons, Institutes and Platforms

    • Manchester Institute of Biotechnology

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