A monoclonal antibody against DNA binding helix of p53 protein

Esma Yolcu; Berna S. Sayan; Tamer Yaǎci; Rengul Cetin-Atalay; Thierry Soussi; Nevzat Yurdusev; Mehmet Ozturk

doi:10.1038/sj.onc.1204240

A monoclonal antibody against DNA binding helix of p53 protein

Esma Yolcu
, Berna S. Sayan
, Tamer Yaǎci
, Rengul Cetin-Atalay
, Thierry Soussi
, Nevzat Yurdusev
, Mehmet Ozturk

Research output: Contribution to journal › Article › peer-review

Abstract

Three monoclonal antibodies (Mabs) were generated against p53 DNA-binding core domain. When tested by immunoprecipitation, Western blot and immunofluorescence techniques, Mab 9E4, as well as 7D3 and 6B10 reacted with both wild-type and various mutant p53 proteins. The epitopes recognized by Mabs 7D3, 9E4 and 6B10 were located respectively within the amino acid residues 211-220, 281-290 and 291-300 of human p53 protein. The epitope recognized by 9E4 Mab coincides with helix 2, also called p53 DNA binding helix, which allows the direct contact of the protein with its target DNA sequences. This antibody may be useful to study transcription-dependent and transcription-independent activities of wild-type and mutant p53 proteins.

Original language	English
Pages (from-to)	1398-1401
Number of pages	3
Journal	Oncogene
Volume	20
Issue number	11
DOIs	https://doi.org/10.1038/sj.onc.1204240
Publication status	Published - 15 Mar 2001

Keywords

DNA binding helix
Hybridoma
p53

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/sj.onc.1204240

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title = "A monoclonal antibody against DNA binding helix of p53 protein",

abstract = "Three monoclonal antibodies (Mabs) were generated against p53 DNA-binding core domain. When tested by immunoprecipitation, Western blot and immunofluorescence techniques, Mab 9E4, as well as 7D3 and 6B10 reacted with both wild-type and various mutant p53 proteins. The epitopes recognized by Mabs 7D3, 9E4 and 6B10 were located respectively within the amino acid residues 211-220, 281-290 and 291-300 of human p53 protein. The epitope recognized by 9E4 Mab coincides with helix 2, also called p53 DNA binding helix, which allows the direct contact of the protein with its target DNA sequences. This antibody may be useful to study transcription-dependent and transcription-independent activities of wild-type and mutant p53 proteins.",

keywords = "DNA binding helix, Hybridoma, p53",

author = "Esma Yolcu and Sayan, \{Berna S.\} and Tamer Yaǎci and Rengul Cetin-Atalay and Thierry Soussi and Nevzat Yurdusev and Mehmet Ozturk",

year = "2001",

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doi = "10.1038/sj.onc.1204240",

language = "English",

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journal = "Oncogene",

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TY - JOUR

T1 - A monoclonal antibody against DNA binding helix of p53 protein

AU - Yolcu, Esma

AU - Sayan, Berna S.

AU - Yaǎci, Tamer

AU - Cetin-Atalay, Rengul

AU - Soussi, Thierry

AU - Yurdusev, Nevzat

AU - Ozturk, Mehmet

PY - 2001/3/15

Y1 - 2001/3/15

N2 - Three monoclonal antibodies (Mabs) were generated against p53 DNA-binding core domain. When tested by immunoprecipitation, Western blot and immunofluorescence techniques, Mab 9E4, as well as 7D3 and 6B10 reacted with both wild-type and various mutant p53 proteins. The epitopes recognized by Mabs 7D3, 9E4 and 6B10 were located respectively within the amino acid residues 211-220, 281-290 and 291-300 of human p53 protein. The epitope recognized by 9E4 Mab coincides with helix 2, also called p53 DNA binding helix, which allows the direct contact of the protein with its target DNA sequences. This antibody may be useful to study transcription-dependent and transcription-independent activities of wild-type and mutant p53 proteins.

AB - Three monoclonal antibodies (Mabs) were generated against p53 DNA-binding core domain. When tested by immunoprecipitation, Western blot and immunofluorescence techniques, Mab 9E4, as well as 7D3 and 6B10 reacted with both wild-type and various mutant p53 proteins. The epitopes recognized by Mabs 7D3, 9E4 and 6B10 were located respectively within the amino acid residues 211-220, 281-290 and 291-300 of human p53 protein. The epitope recognized by 9E4 Mab coincides with helix 2, also called p53 DNA binding helix, which allows the direct contact of the protein with its target DNA sequences. This antibody may be useful to study transcription-dependent and transcription-independent activities of wild-type and mutant p53 proteins.

KW - DNA binding helix

KW - Hybridoma

KW - p53

U2 - 10.1038/sj.onc.1204240

DO - 10.1038/sj.onc.1204240

M3 - Article

C2 - 11313883

SN - 0950-9232

VL - 20

SP - 1398

EP - 1401

JO - Oncogene

JF - Oncogene

IS - 11

ER -

A monoclonal antibody against DNA binding helix of p53 protein

Abstract

Keywords

UN SDGs

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