TY - JOUR
T1 - A multiscale modelling approach for Haematococcus pluvialis cultivation under different environmental conditions
AU - Usai, Alessandro
AU - Pittman, Jon K.
AU - Theodoropoulos, Constantinos
N1 - Funding Information:
C.T. and J.K.P. would like to acknowledge support from the European project EnhanceMicroAlgae , funded by Interreg Atlantic area ( EAPA_338/2016 ).
Publisher Copyright:
© 2022
© 2022 The Authors. Published by Elsevier B.V.
PY - 2022/12
Y1 - 2022/12
N2 - Haematococcus pluvialis can produce significant amounts of industrially important compounds belonging to lipids and starch classes, including various specific pigments such as β-carotene, lutein and astaxanthin, as well as lipids, carbohydrates and proteins. Their production can vary depending on environmental stress conditions like nutrient starvation. However, stress conditions lead also to undesired phenomena such as cell lysis, which is likely to be related to products loss. The microorganism develops towards smaller single cell volumes during the growth process, and eventually, more likely towards lysis when fission (i.e. cell division) slows down. The lysis process takes place simultaneously with nutrient depletion, so both growth and lysis are linked to the change of environmental conditions. In this work, we develop a novel multiscale segregated-structured model based on Population Balance Equations (PBEs) to describe the photoautotrophic growth of H.pluvialis, in particular cell growth, and lysis, making possible the description of the relationship between cell volume/transition, cell loss, and metabolic product availability. Cell volume is the internal coordinate of the population balance model, and its link with intrinsic concentrations is also presented. The model parameters are fitted against experimental data, extensive sensitivity analysis is performed and the model predictive capabilities are tested in terms of cell density distributions, as well as 0th and 1st order moments.
AB - Haematococcus pluvialis can produce significant amounts of industrially important compounds belonging to lipids and starch classes, including various specific pigments such as β-carotene, lutein and astaxanthin, as well as lipids, carbohydrates and proteins. Their production can vary depending on environmental stress conditions like nutrient starvation. However, stress conditions lead also to undesired phenomena such as cell lysis, which is likely to be related to products loss. The microorganism develops towards smaller single cell volumes during the growth process, and eventually, more likely towards lysis when fission (i.e. cell division) slows down. The lysis process takes place simultaneously with nutrient depletion, so both growth and lysis are linked to the change of environmental conditions. In this work, we develop a novel multiscale segregated-structured model based on Population Balance Equations (PBEs) to describe the photoautotrophic growth of H.pluvialis, in particular cell growth, and lysis, making possible the description of the relationship between cell volume/transition, cell loss, and metabolic product availability. Cell volume is the internal coordinate of the population balance model, and its link with intrinsic concentrations is also presented. The model parameters are fitted against experimental data, extensive sensitivity analysis is performed and the model predictive capabilities are tested in terms of cell density distributions, as well as 0th and 1st order moments.
KW - Batch cultivation
KW - Cell fission
KW - Cell lysis
KW - Microalgae
KW - Nutrient limitation
KW - Population balance
UR - http://www.scopus.com/inward/record.url?scp=85140929744&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/b5750528-162f-3615-9cbf-cbad67e9b0df/
U2 - 10.1016/j.btre.2022.e00771
DO - 10.1016/j.btre.2022.e00771
M3 - Article
C2 - 36345543
SN - 2215-017X
VL - 36
SP - e00771
JO - Biotechnology Reports
JF - Biotechnology Reports
M1 - e00771
ER -