A mutant defective in sexual development produces aseptate ascogonia

Sandra Bloemendal, Kathryn M. Lord, Christine Rech, Birgit Hoff, Ines Engh, Nick D. Read, Ulrich Kück

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The transition from the vegetative to the sexual cycle in filamentous ascomycetes is initiated with the formation of ascogonia. Here, we describe a novel type of sterile mutant from Sordaria macrospora with a defect in ascogonial septum formation. This mutant, named pro22, produces only small, defective protoperithecia and carries a point mutation in a gene encoding a protein that is highly conserved throughout eukaryotes. Sequence analyses revealed three putative transmembrane domains and a C-terminal domain of unknown function. Live-cell imaging showed that PRO22 is predominantly localized in the dynamic tubular and vesicular vacuolar network of the peripheral colony region close to growing hyphal tips and in ascogonia; it is absent from the large spherical vacuoles in the vegetative hyphae of the subperipheral region of the colony. This points to a specific role of PRO22 in the tubular and vesicular vacuolar network, and the loss of intercalary septation in ascogonia suggests that PRO22 functions during the initiation of sexual development. © 2010, American Society for Microbiology. All Rights Reserved.
    Original languageEnglish
    Pages (from-to)1856-1866
    Number of pages10
    JournalEukaryotic Cell
    Volume9
    Issue number12
    DOIs
    Publication statusPublished - Dec 2010

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