TY - JOUR
T1 - A neural survival factor is a candidate oncogene in breast cancer
AU - Porter, Dale
AU - Weremowicz, Stanislawa
AU - Chin, Koei
AU - Seth, Pankaj
AU - Keshaviah, Aparna
AU - Lahti-Domenici, Jaana
AU - Bae, Young Kyung
AU - Monitto, Constance L
AU - Merlos-Suarez, Ana
AU - Chan, Jennifer
AU - Hulette, Christine M
AU - Richardson, Andrea
AU - Morton, Cynthia C
AU - Marks, Jeffrey
AU - Duyao, Mabel
AU - Hruban, Ralph
AU - Gabrielson, Edward
AU - Gelman, Rebecca
AU - Polyak, Kornelia
PY - 2003/9/16
Y1 - 2003/9/16
N2 - Using serial analysis of gene expression (SAGE), we identified a SAGE tag that was present only in invasive breast carcinomas and their lymph node metastases. The transcript corresponding to this SAGE tag, dermcidin (DCD), encodes a secreted protein normally expressed only in the pons of the brain and sweat glands. Array comparative genomic hybridization, fluorescence in situ hybridization, and immunohistochemical analyses determined that DCD is overexpressed in approximately 10% of invasive breast carcinomas; in some cases its overexpression is coupled with a focal copy number gain of its locus at 12q13.1, and its expression is associated with advanced clinical stage and poor prognosis. Expression of DCD in breast cancer cells promotes cell growth and survival and reduces serum dependency. Putative high- and low-affinity receptors for DCD are present on the cell surface of breast carcinomas and neurons of the brain. Based on these data we hypothesize that DCD may play a role in tumorigenesis by means of enhancing cell growth and survival in a subset of breast carcinomas.
AB - Using serial analysis of gene expression (SAGE), we identified a SAGE tag that was present only in invasive breast carcinomas and their lymph node metastases. The transcript corresponding to this SAGE tag, dermcidin (DCD), encodes a secreted protein normally expressed only in the pons of the brain and sweat glands. Array comparative genomic hybridization, fluorescence in situ hybridization, and immunohistochemical analyses determined that DCD is overexpressed in approximately 10% of invasive breast carcinomas; in some cases its overexpression is coupled with a focal copy number gain of its locus at 12q13.1, and its expression is associated with advanced clinical stage and poor prognosis. Expression of DCD in breast cancer cells promotes cell growth and survival and reduces serum dependency. Putative high- and low-affinity receptors for DCD are present on the cell surface of breast carcinomas and neurons of the brain. Based on these data we hypothesize that DCD may play a role in tumorigenesis by means of enhancing cell growth and survival in a subset of breast carcinomas.
KW - Amino Acid Sequence
KW - Base Sequence
KW - Breast Neoplasms
KW - DNA, Neoplasm
KW - Gene Expression Profiling
KW - Humans
KW - Molecular Sequence Data
KW - Neoplasm Invasiveness
KW - Oncogenes
KW - Sequence Homology, Amino Acid
KW - Tumor Cells, Cultured
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
KW - Research Support, U.S. Gov't, Non-P.H.S.
KW - Research Support, U.S. Gov't, P.H.S.
U2 - 10.1073/pnas.1932980100
DO - 10.1073/pnas.1932980100
M3 - Article
C2 - 12953101
SN - 0027-8424
VL - 100
SP - 10931
EP - 10936
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 19
ER -
