A new luminescence lifetime macro-imager based on Tpx3Cam optical camera

Rajannya Sen, Liisa M. Hirvonen, Alexander V. Zhdanov, Peter Svihra, Stefan Andersson-Engels, Andrei Nomerotski, Dmitri B. Papkovsky

Research output: Contribution to journalArticlepeer-review

108 Downloads (Pure)


Properties of a novel ultra-fast optical imager, Tpx3Cam, were investigated for macroscopic wide-field phosphorescent lifetime imaging (PLIM) applications. The camera is based on a novel optical sensor and Timepix3 readout chip with time resolution of 1.6 ns, recording of photon arrival time and time over threshold for each pixel, and readout rate of 80 megapixels per second. In this study, we coupled the camera to an image intensifier, a 760 nm emission filter and a 50 mm lens, and with a super-bright 625nm LED providing pulsed excitation of a 18x18mm sample area. The resulting macro-imager with compact and rigid optical alignment of its main components was characterised using planar phosphorescent O2 sensors and a resolution plate mask. Several acquisition and image processing algorithms were evaluated to optimise the system resolution and performance for the wide-field PLIM, followed by imaging a variety of phosphorescent samples. The new PLIM system looks promising, particularly for phosphorescence lifetime-based imaging of O2 in various chemical and biological samples.
Original languageEnglish
Number of pages9
JournalBiomedical Optics Express
Issue number1
Publication statusPublished - 1 Jan 2020


  • Phosphorescence Lifetime IMaging (PLIM)
  • Fluorescence Lifetime IMaging (FLIM)
  • Time Correlated Single Photon Counting (TCSPC)
  • Macroscopic imaging
  • Oxygen sensing and imaging
  • Tpx3Cam


Dive into the research topics of 'A new luminescence lifetime macro-imager based on Tpx3Cam optical camera'. Together they form a unique fingerprint.

Cite this