A non-enzymatic, dna template-directed morpholino primer extension approach

Neil M. Bell, Raymond Wong, Jason Micklefield

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Extension, please ! A non-enzymatic DNA-template-directed synthesis approach has been developed that allows the morpholino-nucleoside extension of a PNA primer. Equilibration of the ribonucleosides with the template-primer complex, in a noncovalent fashion (see scheme), was shown to be essential for high selectivity. It is envisaged that this chemistry might be used to transcribe nature's genetic material into alternative abiological polymers. (Chemical Equation Representation). © 2010 Wiley-VCH Verlag GmbH & Co. KGaA,.
    Original languageEnglish
    Pages (from-to)2026-2030
    Number of pages4
    JournalChemistry - A European Journal
    Volume16
    Issue number7
    DOIs
    Publication statusPublished - 15 Feb 2010

    Keywords

    • DNA
    • Morpholino
    • Non-enzymatic
    • PNA
    • Primer extension
    • Ribonucleosides

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