A non-enzymatic, dna template-directed morpholino primer extension approach

Neil M. Bell; Raymond Wong; Jason Micklefield

doi:10.1002/chem.200902237

A non-enzymatic, dna template-directed morpholino primer extension approach

Neil M. Bell, Raymond Wong, Jason Micklefield

Research output: Contribution to journal › Article › peer-review

Abstract

Extension, please ! A non-enzymatic DNA-template-directed synthesis approach has been developed that allows the morpholino-nucleoside extension of a PNA primer. Equilibration of the ribonucleosides with the template-primer complex, in a noncovalent fashion (see scheme), was shown to be essential for high selectivity. It is envisaged that this chemistry might be used to transcribe nature's genetic material into alternative abiological polymers. (Chemical Equation Representation). © 2010 Wiley-VCH Verlag GmbH & Co. KGaA,.

Original language	English
Pages (from-to)	2026-2030
Number of pages	4
Journal	Chemistry - A European Journal
Volume	16
Issue number	7
DOIs	https://doi.org/10.1002/chem.200902237
Publication status	Published - 15 Feb 2010

Keywords

DNA
Morpholino
Non-enzymatic
PNA
Primer extension
Ribonucleosides

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10.1002/chem.200902237

http://www3.interscience.wiley.com/cgi-bin/fulltext/123244370/PDFSTART

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title = "A non-enzymatic, dna template-directed morpholino primer extension approach",

abstract = "Extension, please ! A non-enzymatic DNA-template-directed synthesis approach has been developed that allows the morpholino-nucleoside extension of a PNA primer. Equilibration of the ribonucleosides with the template-primer complex, in a noncovalent fashion (see scheme), was shown to be essential for high selectivity. It is envisaged that this chemistry might be used to transcribe nature's genetic material into alternative abiological polymers. (Chemical Equation Representation). {\textcopyright} 2010 Wiley-VCH Verlag GmbH & Co. KGaA,.",

keywords = "DNA, Morpholino, Non-enzymatic, PNA, Primer extension, Ribonucleosides",

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doi = "10.1002/chem.200902237",

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AU - Bell, Neil M.

AU - Wong, Raymond

AU - Micklefield, Jason

PY - 2010/2/15

Y1 - 2010/2/15

N2 - Extension, please ! A non-enzymatic DNA-template-directed synthesis approach has been developed that allows the morpholino-nucleoside extension of a PNA primer. Equilibration of the ribonucleosides with the template-primer complex, in a noncovalent fashion (see scheme), was shown to be essential for high selectivity. It is envisaged that this chemistry might be used to transcribe nature's genetic material into alternative abiological polymers. (Chemical Equation Representation). © 2010 Wiley-VCH Verlag GmbH & Co. KGaA,.

AB - Extension, please ! A non-enzymatic DNA-template-directed synthesis approach has been developed that allows the morpholino-nucleoside extension of a PNA primer. Equilibration of the ribonucleosides with the template-primer complex, in a noncovalent fashion (see scheme), was shown to be essential for high selectivity. It is envisaged that this chemistry might be used to transcribe nature's genetic material into alternative abiological polymers. (Chemical Equation Representation). © 2010 Wiley-VCH Verlag GmbH & Co. KGaA,.

KW - DNA

KW - Morpholino

KW - Non-enzymatic

KW - PNA

KW - Primer extension

KW - Ribonucleosides

U2 - 10.1002/chem.200902237

DO - 10.1002/chem.200902237

M3 - Article

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JO - Chemistry: A European Journal

JF - Chemistry: A European Journal

SN - 0947-6539

IS - 7

ER -

A non-enzymatic, dna template-directed morpholino primer extension approach

Abstract

Keywords

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