A novel experimental system of high stability and lifetime for the laser-desorption of biomolecules

Mehran Taherkhani; Mikko Riese; Mohammed Benyezzar; Klaus Müller-Dethlefs

doi:10.1063/1.3373977

A novel experimental system of high stability and lifetime for the laser-desorption of biomolecules

Mehran Taherkhani, Mikko Riese, Mohammed Benyezzar, Klaus Müller-Dethlefs

Research output: Contribution to journal › Article › peer-review

Abstract

A novel laser desorption system, with improved signal stability and extraordinary long lifetime, is presented for the study of jet-cooled biomolecules in the gas phase using vibrationally resolved photoionization spectroscopy. As a test substance tryptophane is used to characterize this desorption source. A usable lifetime of above 1 month (for a laser desorption repetition rate of 20 Hz) has been observed by optimizing the pellets (graphite/tryptophane, 3 mm diameter and 6 mm length) from which the substance is laser-desorbed. Additionally, the stability and signal-to-noise ratio has been improved by averaging the signal over the entire sample pellet by synchronizing the data acquisition with the rotation of the sample rod. The results demonstrate how a combination of the above helps to produce stable and conclusive spectra of tryptophane using one-color and two-color resonant two-photon ionization studies. © 2010 American Institute of Physics.

Original language	English
Article number	063101
Journal	Review of Scientific Instruments
Volume	81
Issue number	6
DOIs	https://doi.org/10.1063/1.3373977
Publication status	Published - Jun 2010

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title = "A novel experimental system of high stability and lifetime for the laser-desorption of biomolecules",

abstract = "A novel laser desorption system, with improved signal stability and extraordinary long lifetime, is presented for the study of jet-cooled biomolecules in the gas phase using vibrationally resolved photoionization spectroscopy. As a test substance tryptophane is used to characterize this desorption source. A usable lifetime of above 1 month (for a laser desorption repetition rate of 20 Hz) has been observed by optimizing the pellets (graphite/tryptophane, 3 mm diameter and 6 mm length) from which the substance is laser-desorbed. Additionally, the stability and signal-to-noise ratio has been improved by averaging the signal over the entire sample pellet by synchronizing the data acquisition with the rotation of the sample rod. The results demonstrate how a combination of the above helps to produce stable and conclusive spectra of tryptophane using one-color and two-color resonant two-photon ionization studies. {\textcopyright} 2010 American Institute of Physics.",

author = "Mehran Taherkhani and Mikko Riese and Mohammed Benyezzar and Klaus M{\"u}ller-Dethlefs",

note = "Taherkhani, Mehran Riese, Mikko BenYezzar, Mohammed Mueller-Dethlefs, Klaus",

year = "2010",

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AU - Taherkhani, Mehran

AU - Riese, Mikko

AU - Benyezzar, Mohammed

AU - Müller-Dethlefs, Klaus

N1 - Taherkhani, Mehran Riese, Mikko BenYezzar, Mohammed Mueller-Dethlefs, Klaus

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N2 - A novel laser desorption system, with improved signal stability and extraordinary long lifetime, is presented for the study of jet-cooled biomolecules in the gas phase using vibrationally resolved photoionization spectroscopy. As a test substance tryptophane is used to characterize this desorption source. A usable lifetime of above 1 month (for a laser desorption repetition rate of 20 Hz) has been observed by optimizing the pellets (graphite/tryptophane, 3 mm diameter and 6 mm length) from which the substance is laser-desorbed. Additionally, the stability and signal-to-noise ratio has been improved by averaging the signal over the entire sample pellet by synchronizing the data acquisition with the rotation of the sample rod. The results demonstrate how a combination of the above helps to produce stable and conclusive spectra of tryptophane using one-color and two-color resonant two-photon ionization studies. © 2010 American Institute of Physics.

AB - A novel laser desorption system, with improved signal stability and extraordinary long lifetime, is presented for the study of jet-cooled biomolecules in the gas phase using vibrationally resolved photoionization spectroscopy. As a test substance tryptophane is used to characterize this desorption source. A usable lifetime of above 1 month (for a laser desorption repetition rate of 20 Hz) has been observed by optimizing the pellets (graphite/tryptophane, 3 mm diameter and 6 mm length) from which the substance is laser-desorbed. Additionally, the stability and signal-to-noise ratio has been improved by averaging the signal over the entire sample pellet by synchronizing the data acquisition with the rotation of the sample rod. The results demonstrate how a combination of the above helps to produce stable and conclusive spectra of tryptophane using one-color and two-color resonant two-photon ionization studies. © 2010 American Institute of Physics.

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DO - 10.1063/1.3373977

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VL - 81

JO - Review of Scientific Instruments

JF - Review of Scientific Instruments

IS - 6

M1 - 063101

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A novel experimental system of high stability and lifetime for the laser-desorption of biomolecules

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