A novel method for separation of somatic embryos from embryogenic suspension cultures by cold treatment

Saadet Büyükalaca, Ferda Mavituna, Layla Z A Rahman

    Research output: Contribution to journalArticlepeer-review

    Abstract

    This study reports a novel method for embryo separation by cold treatment of heterogeneous suspension cultures which contain embryogenic single cells, cell clusters and embryos at various stages of development. The method was applied to embryo suspension cultures of pepper (Capsicum annuum) and sugarcane (Saccharum officinarum). In both plants, single cells lost their viability dramatically over a few days while the viability of embryos remained above 95% for 25-30 days when kept at 5°C. The effect of duration of cold treatment on embryo germination was also tested. The optimal duration of cold treatment was found to be 10 days for sugarcane and 21 days for pepper. After the cold treatment, the germination percentages were 90% and 96% for sugarcane and pepper, respectively.
    Original languageEnglish
    Pages (from-to)143-146
    Number of pages3
    JournalJournal of Plant Biochemistry and Biotechnology
    Volume12
    Issue number2
    Publication statusPublished - Jul 2003

    Keywords

    • Capsicum annuum
    • Cold treatment
    • Germination
    • Heat treatment
    • Saccharum officinarum
    • Somatic embryo
    • Synchronisation

    Fingerprint

    Dive into the research topics of 'A novel method for separation of somatic embryos from embryogenic suspension cultures by cold treatment'. Together they form a unique fingerprint.

    Cite this