A novel monoclonal antibody to Neisseria meningitidis serogroup X capsular polysaccharide and its potential use in quantitation of meningococcal vaccines

Fátima Reyes; Oscar Otero; Frank Camacho; Nevis Amin; Fidel Ramirez Bencomo; Yolanda Valdés; Reynaldo Acevedo; Luis García; Daniel Cardoso; Maribel Cuello

doi:10.1016/j.biologicals.2014.08.001

A novel monoclonal antibody to Neisseria meningitidis serogroup X capsular polysaccharide and its potential use in quantitation of meningococcal vaccines

Fátima Reyes^*, Oscar Otero, Frank Camacho, Nevis Amin, Fidel Ramirez Bencomo, Yolanda Valdés, Reynaldo Acevedo, Luis García, Daniel Cardoso, Maribel Cuello

^*Corresponding author for this work

Division of Evolution, Infection and Genomics

Research output: Contribution to journal › Article › peer-review

Abstract

A novel murine hybridoma monoclonal antibody (MAb) was produced against the capsular polysaccharide (CP) of Neisseria meningitidis serogroup X (MenX) in order to develop a sandwich enzyme linked immunosorbent assay (ELISA) for the quantitation of the meningococcal polysaccharide. The MAb only reacted with the CP from MenX and did not react with CPs from N. meningitidis serogroups A, C, Y and W (MenA, MenC, MenY, MenW). The affinity constant (Ka) of the MAb measured by non-competitive ELISA was 7.25 × 107 M−1. The application of this MAb in a sandwich ELISA was demonstrated by its ability to properly quantitate three lots of an experimental meningococcal CP-based vaccine. The MAb obtained in this work could be a valuable reagent for the detection and quantitation of future meningococcal vaccines containing MenX CP.

Original language	English
Pages (from-to)	312-315
Number of pages	4
Journal	Biologicals
Volume	42
Issue number	6
DOIs	https://doi.org/10.1016/j.biologicals.2014.08.001
Publication status	Published - 2014

Keywords

monoclonal antibody
Neisseria meningitidis serogroup X
ELISA
quantitation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biologicals.2014.08.001

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Reyes, F., Otero, O., Camacho, F., Amin, N., Ramirez Bencomo, F., Valdés, Y., Acevedo, R., García, L., Cardoso, D., & Cuello, M. (2014). A novel monoclonal antibody to Neisseria meningitidis serogroup X capsular polysaccharide and its potential use in quantitation of meningococcal vaccines. Biologicals, 42(6), 312-315. https://doi.org/10.1016/j.biologicals.2014.08.001

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title = "A novel monoclonal antibody to Neisseria meningitidis serogroup X capsular polysaccharide and its potential use in quantitation of meningococcal vaccines",

abstract = "A novel murine hybridoma monoclonal antibody (MAb) was produced against the capsular polysaccharide (CP) of Neisseria meningitidis serogroup X (MenX) in order to develop a sandwich enzyme linked immunosorbent assay (ELISA) for the quantitation of the meningococcal polysaccharide. The MAb only reacted with the CP from MenX and did not react with CPs from N. meningitidis serogroups A, C, Y and W (MenA, MenC, MenY, MenW). The affinity constant (Ka) of the MAb measured by non-competitive ELISA was 7.25 × 107 M−1. The application of this MAb in a sandwich ELISA was demonstrated by its ability to properly quantitate three lots of an experimental meningococcal CP-based vaccine. The MAb obtained in this work could be a valuable reagent for the detection and quantitation of future meningococcal vaccines containing MenX CP.",

keywords = "monoclonal antibody, Neisseria meningitidis serogroup X, ELISA, quantitation",

author = "F{\'a}tima Reyes and Oscar Otero and Frank Camacho and Nevis Amin and {Ramirez Bencomo}, Fidel and Yolanda Vald{\'e}s and Reynaldo Acevedo and Luis Garc{\'i}a and Daniel Cardoso and Maribel Cuello",

year = "2014",

doi = "10.1016/j.biologicals.2014.08.001",

language = "English",

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Reyes, F, Otero, O, Camacho, F, Amin, N, Ramirez Bencomo, F, Valdés, Y, Acevedo, R, García, L, Cardoso, D & Cuello, M 2014, 'A novel monoclonal antibody to Neisseria meningitidis serogroup X capsular polysaccharide and its potential use in quantitation of meningococcal vaccines', Biologicals, vol. 42, no. 6, pp. 312-315. https://doi.org/10.1016/j.biologicals.2014.08.001

TY - JOUR

T1 - A novel monoclonal antibody to Neisseria meningitidis serogroup X capsular polysaccharide and its potential use in quantitation of meningococcal vaccines

AU - Reyes, Fátima

AU - Otero, Oscar

AU - Camacho, Frank

AU - Amin, Nevis

AU - Ramirez Bencomo, Fidel

AU - Valdés, Yolanda

AU - Acevedo, Reynaldo

AU - García, Luis

AU - Cardoso, Daniel

AU - Cuello, Maribel

PY - 2014

Y1 - 2014

N2 - A novel murine hybridoma monoclonal antibody (MAb) was produced against the capsular polysaccharide (CP) of Neisseria meningitidis serogroup X (MenX) in order to develop a sandwich enzyme linked immunosorbent assay (ELISA) for the quantitation of the meningococcal polysaccharide. The MAb only reacted with the CP from MenX and did not react with CPs from N. meningitidis serogroups A, C, Y and W (MenA, MenC, MenY, MenW). The affinity constant (Ka) of the MAb measured by non-competitive ELISA was 7.25 × 107 M−1. The application of this MAb in a sandwich ELISA was demonstrated by its ability to properly quantitate three lots of an experimental meningococcal CP-based vaccine. The MAb obtained in this work could be a valuable reagent for the detection and quantitation of future meningococcal vaccines containing MenX CP.

AB - A novel murine hybridoma monoclonal antibody (MAb) was produced against the capsular polysaccharide (CP) of Neisseria meningitidis serogroup X (MenX) in order to develop a sandwich enzyme linked immunosorbent assay (ELISA) for the quantitation of the meningococcal polysaccharide. The MAb only reacted with the CP from MenX and did not react with CPs from N. meningitidis serogroups A, C, Y and W (MenA, MenC, MenY, MenW). The affinity constant (Ka) of the MAb measured by non-competitive ELISA was 7.25 × 107 M−1. The application of this MAb in a sandwich ELISA was demonstrated by its ability to properly quantitate three lots of an experimental meningococcal CP-based vaccine. The MAb obtained in this work could be a valuable reagent for the detection and quantitation of future meningococcal vaccines containing MenX CP.

KW - monoclonal antibody

KW - Neisseria meningitidis serogroup X

KW - ELISA

KW - quantitation

U2 - 10.1016/j.biologicals.2014.08.001

DO - 10.1016/j.biologicals.2014.08.001

M3 - Article

C2 - 25218518

SN - 1045-1056

VL - 42

SP - 312

EP - 315

JO - Biologicals

JF - Biologicals

IS - 6

ER -

A novel monoclonal antibody to Neisseria meningitidis serogroup X capsular polysaccharide and its potential use in quantitation of meningococcal vaccines

Abstract

Keywords

UN SDGs

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