A 'Plug and Play' Platform for the Production of Diverse Monoterpene Hydrocarbon Scaffolds in Escherichia coli

Nicole Leferink; Adrian Jervis; Ziga Zebec; Helen Toogood; Sam Hay; Eriko Takano; Nigel Scrutton

doi:10.1002/slct.201600563

A 'Plug and Play' Platform for the Production of Diverse Monoterpene Hydrocarbon Scaffolds in Escherichia coli

Nicole Leferink, Adrian Jervis, Ziga Zebec, Helen Toogood, Sam Hay, Eriko Takano, Nigel Scrutton

Research output: Contribution to journal › Article › peer-review

Abstract

The terpenoids constitute one of the largest and most diverse classes of natural compounds with applications as pharmaceuticals, flavorings and fragrances, pesticides and biofuels. Synthetic biology is ideally placed to create new routes to this chemical diversity and facilitation of new compound discovery. The C10 monoterpenoids display a huge structural diversity produced from a single substrate, geranyl diphosphate, by a family of monoterpene cyclases and synthases (mTC/S). Here we employ a library of mTC/S in a single ‘plug and play’ platform system for the production of over 30 different monoterpenoids in Escherichia coli by fermentation on glucose. These products include several compounds never before produced in engineered microbes demonstrating the power of this approach to rapidly create routes to structural diversity.

Original language	English
Pages (from-to)	1893-1896
Number of pages	4
Journal	ChemistrySelect
Volume	1
Issue number	9
Early online date	21 Jun 2016
DOIs	https://doi.org/10.1002/slct.201600563
Publication status	Published - Jun 2016

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10.1002/slct.201600563Licence: CC BY

Japan Partnering Award: Application of MS-Imaging and Metabolomics in Synthetic Biology Based Strain Improvement of Industrially Important Microbes
Takano, E., Breitling, R. & Hollywood, K.
1/07/16 → 30/06/20
Project: Research
Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals
Scrutton, N., Azapagic, A., Balmer, A., Barran, P., Breitling, R., Delneri, D., Dixon, N., Faulon, J., Flitsch, S., Goble, C., Goodacre, R., Hay, S., Kell, D., Leys, D., Lloyd, J., Lockyer, N., Martin, P., Micklefield, J., Munro, A., Pedrosa Mendes, P., Randles, S., Salehi Yazdi, F., Shapira, P., Takano, E., Turner, N. & Winterburn, J.
14/11/14 → 13/05/20
Project: Research
Innovative routes to monoterpene hydrocarbons and their high value derivatives
Scrutton, N., Breitling, R., Gardiner, J., Hay, S., Leys, D., Pedrosa Mendes, P. & Takano, E.
1/11/14 → 30/04/20
Project: Research

Cite this

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abstract = "The terpenoids constitute one of the largest and most diverse classes of natural compounds with applications as pharmaceuticals, flavorings and fragrances, pesticides and biofuels. Synthetic biology is ideally placed to create new routes to this chemical diversity and facilitation of new compound discovery. The C10 monoterpenoids display a huge structural diversity produced from a single substrate, geranyl diphosphate, by a family of monoterpene cyclases and synthases (mTC/S). Here we employ a library of mTC/S in a single {\textquoteleft}plug and play{\textquoteright} platform system for the production of over 30 different monoterpenoids in Escherichia coli by fermentation on glucose. These products include several compounds never before produced in engineered microbes demonstrating the power of this approach to rapidly create routes to structural diversity.",

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AU - Hay, Sam

AU - Takano, Eriko

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A 'Plug and Play' Platform for the Production of Diverse Monoterpene Hydrocarbon Scaffolds in Escherichia coli

Abstract

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Projects

Japan Partnering Award: Application of MS-Imaging and Metabolomics in Synthetic Biology Based Strain Improvement of Industrially Important Microbes

Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals

Innovative routes to monoterpene hydrocarbons and their high value derivatives

Cite this