A prime-boost immunisation regimen using DNA followed by recombinant modified vaccinia virus Ankara induces strong cellular immune responses against the Plasmodium falciparum TRAP antigen in chimpanzees

Joerg Schneider; Jan A M Langermans; Sarah C. Gilbert; Tom J. Blanchard; Stephen Twigg; Silvia Naitza; Carolyn M. Hannan; Michael Aidoo; Andrea Crisanti; Kathryn J. Robson; Geoffrey L. Smith; Adrian V S Hill; Alan W. Thomas

doi:10.1016/S0264-410X(01)00260-2

A prime-boost immunisation regimen using DNA followed by recombinant modified vaccinia virus Ankara induces strong cellular immune responses against the Plasmodium falciparum TRAP antigen in chimpanzees

Joerg Schneider, Jan A M Langermans, Sarah C. Gilbert, Tom J. Blanchard, Stephen Twigg, Silvia Naitza, Carolyn M. Hannan, Michael Aidoo, Andrea Crisanti, Kathryn J. Robson, Geoffrey L. Smith, Adrian V S Hill, Alan W. Thomas

Research output: Contribution to journal › Article › peer-review

Abstract

Two chimpanzees were vaccinated intramuscularly against malaria using plasmid DNA expressing the pre-erythrocytic antigens thrombospondin related adhesion protein (PfTRAP) and liver stage specific antigen-1 (PfLSA-1) of Plasmodium falciparum together with GM-CSF protein. A recombinant modified vaccinia virus Ankara (MVA) expressing PfTRAP was injected intramuscularly 6 weeks later to boost the immune response. This sequence of antigen delivery induced a specific and long-lasting T cell and antibody response to PfTRAP as detected by ELISPOT assay and ELISA. Antibody responses were detected after four DNA injections, and were boosted by injection of recombinant MVA expressing PfTRAP. Interferon-gamma secreting antigen-specific T cells were detected in both animals, but only after boosting with recombinant MVA. By screening a panel of PfTRAP-derived peptides, an epitope was identified that was recognized by cytotoxic T lymphocytes in one of the chimpanzees studied. T cells specific for this epitope were present in PBMCs and liver-infiltrating lymphocytes at a frequency of between 1 in 200 and 1 in 500. The high immunogenicity of this prime-boost regimen in chimpanzees supports further assessment of this delivery strategy for the induction of protection against P. falciparum malaria in humans. © 2001 Elsevier Science Ltd. All rights reserved.

Original language	English
Pages (from-to)	4595-4602
Number of pages	7
Journal	Vaccine
Volume	19
Issue number	32
DOIs	https://doi.org/10.1016/S0264-410X(01)00260-2
Publication status	Published - 14 Sept 2001

Keywords

Malaria
Prime-boost
Vaccine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0264-410X(01)00260-2

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Schneider, J., Langermans, J. A. M., Gilbert, S. C., Blanchard, T. J., Twigg, S., Naitza, S., Hannan, C. M., Aidoo, M., Crisanti, A., Robson, K. J., Smith, G. L., Hill, A. V. S., & Thomas, A. W. (2001). A prime-boost immunisation regimen using DNA followed by recombinant modified vaccinia virus Ankara induces strong cellular immune responses against the Plasmodium falciparum TRAP antigen in chimpanzees. Vaccine, 19(32), 4595-4602. https://doi.org/10.1016/S0264-410X(01)00260-2

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title = "A prime-boost immunisation regimen using DNA followed by recombinant modified vaccinia virus Ankara induces strong cellular immune responses against the Plasmodium falciparum TRAP antigen in chimpanzees",

abstract = "Two chimpanzees were vaccinated intramuscularly against malaria using plasmid DNA expressing the pre-erythrocytic antigens thrombospondin related adhesion protein (PfTRAP) and liver stage specific antigen-1 (PfLSA-1) of Plasmodium falciparum together with GM-CSF protein. A recombinant modified vaccinia virus Ankara (MVA) expressing PfTRAP was injected intramuscularly 6 weeks later to boost the immune response. This sequence of antigen delivery induced a specific and long-lasting T cell and antibody response to PfTRAP as detected by ELISPOT assay and ELISA. Antibody responses were detected after four DNA injections, and were boosted by injection of recombinant MVA expressing PfTRAP. Interferon-gamma secreting antigen-specific T cells were detected in both animals, but only after boosting with recombinant MVA. By screening a panel of PfTRAP-derived peptides, an epitope was identified that was recognized by cytotoxic T lymphocytes in one of the chimpanzees studied. T cells specific for this epitope were present in PBMCs and liver-infiltrating lymphocytes at a frequency of between 1 in 200 and 1 in 500. The high immunogenicity of this prime-boost regimen in chimpanzees supports further assessment of this delivery strategy for the induction of protection against P. falciparum malaria in humans. {\textcopyright} 2001 Elsevier Science Ltd. All rights reserved.",

keywords = "Malaria, Prime-boost, Vaccine",

author = "Joerg Schneider and Langermans, {Jan A M} and Gilbert, {Sarah C.} and Blanchard, {Tom J.} and Stephen Twigg and Silvia Naitza and Hannan, {Carolyn M.} and Michael Aidoo and Andrea Crisanti and Robson, {Kathryn J.} and Smith, {Geoffrey L.} and Hill, {Adrian V S} and Thomas, {Alan W.}",

year = "2001",

month = sep,

day = "14",

doi = "10.1016/S0264-410X(01)00260-2",

language = "English",

volume = "19",

pages = "4595--4602",

journal = "Vaccine",

issn = "1873-2518",

publisher = "Elsevier BV",

number = "32",

}

Schneider, J, Langermans, JAM, Gilbert, SC, Blanchard, TJ, Twigg, S, Naitza, S, Hannan, CM, Aidoo, M, Crisanti, A, Robson, KJ, Smith, GL, Hill, AVS & Thomas, AW 2001, 'A prime-boost immunisation regimen using DNA followed by recombinant modified vaccinia virus Ankara induces strong cellular immune responses against the Plasmodium falciparum TRAP antigen in chimpanzees', Vaccine, vol. 19, no. 32, pp. 4595-4602. https://doi.org/10.1016/S0264-410X(01)00260-2

A prime-boost immunisation regimen using DNA followed by recombinant modified vaccinia virus Ankara induces strong cellular immune responses against the Plasmodium falciparum TRAP antigen in chimpanzees. / Schneider, Joerg; Langermans, Jan A M; Gilbert, Sarah C. et al.
In: Vaccine, Vol. 19, No. 32, 14.09.2001, p. 4595-4602.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - A prime-boost immunisation regimen using DNA followed by recombinant modified vaccinia virus Ankara induces strong cellular immune responses against the Plasmodium falciparum TRAP antigen in chimpanzees

AU - Schneider, Joerg

AU - Langermans, Jan A M

AU - Gilbert, Sarah C.

AU - Blanchard, Tom J.

AU - Twigg, Stephen

AU - Naitza, Silvia

AU - Hannan, Carolyn M.

AU - Aidoo, Michael

AU - Crisanti, Andrea

AU - Robson, Kathryn J.

AU - Smith, Geoffrey L.

AU - Hill, Adrian V S

AU - Thomas, Alan W.

PY - 2001/9/14

Y1 - 2001/9/14

N2 - Two chimpanzees were vaccinated intramuscularly against malaria using plasmid DNA expressing the pre-erythrocytic antigens thrombospondin related adhesion protein (PfTRAP) and liver stage specific antigen-1 (PfLSA-1) of Plasmodium falciparum together with GM-CSF protein. A recombinant modified vaccinia virus Ankara (MVA) expressing PfTRAP was injected intramuscularly 6 weeks later to boost the immune response. This sequence of antigen delivery induced a specific and long-lasting T cell and antibody response to PfTRAP as detected by ELISPOT assay and ELISA. Antibody responses were detected after four DNA injections, and were boosted by injection of recombinant MVA expressing PfTRAP. Interferon-gamma secreting antigen-specific T cells were detected in both animals, but only after boosting with recombinant MVA. By screening a panel of PfTRAP-derived peptides, an epitope was identified that was recognized by cytotoxic T lymphocytes in one of the chimpanzees studied. T cells specific for this epitope were present in PBMCs and liver-infiltrating lymphocytes at a frequency of between 1 in 200 and 1 in 500. The high immunogenicity of this prime-boost regimen in chimpanzees supports further assessment of this delivery strategy for the induction of protection against P. falciparum malaria in humans. © 2001 Elsevier Science Ltd. All rights reserved.

AB - Two chimpanzees were vaccinated intramuscularly against malaria using plasmid DNA expressing the pre-erythrocytic antigens thrombospondin related adhesion protein (PfTRAP) and liver stage specific antigen-1 (PfLSA-1) of Plasmodium falciparum together with GM-CSF protein. A recombinant modified vaccinia virus Ankara (MVA) expressing PfTRAP was injected intramuscularly 6 weeks later to boost the immune response. This sequence of antigen delivery induced a specific and long-lasting T cell and antibody response to PfTRAP as detected by ELISPOT assay and ELISA. Antibody responses were detected after four DNA injections, and were boosted by injection of recombinant MVA expressing PfTRAP. Interferon-gamma secreting antigen-specific T cells were detected in both animals, but only after boosting with recombinant MVA. By screening a panel of PfTRAP-derived peptides, an epitope was identified that was recognized by cytotoxic T lymphocytes in one of the chimpanzees studied. T cells specific for this epitope were present in PBMCs and liver-infiltrating lymphocytes at a frequency of between 1 in 200 and 1 in 500. The high immunogenicity of this prime-boost regimen in chimpanzees supports further assessment of this delivery strategy for the induction of protection against P. falciparum malaria in humans. © 2001 Elsevier Science Ltd. All rights reserved.

KW - Malaria

KW - Prime-boost

KW - Vaccine

U2 - 10.1016/S0264-410X(01)00260-2

DO - 10.1016/S0264-410X(01)00260-2

M3 - Article

SN - 1873-2518

VL - 19

SP - 4595

EP - 4602

JO - Vaccine

JF - Vaccine

IS - 32

ER -

A prime-boost immunisation regimen using DNA followed by recombinant modified vaccinia virus Ankara induces strong cellular immune responses against the Plasmodium falciparum TRAP antigen in chimpanzees

Abstract

Keywords

UN SDGs

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