A proteomic and phosphoproteomic analysis of Oryza sativa plasma membrane and vacuolar membrane

Sally Anne Whiteman, Thomas S. Nühse, David A. Ashford, Dale Sanders, Frans J M Maathuis

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Proteomic and phosphoproteomic analyses of rice shoot and root tonoplast-enriched and plasma membrane-enriched membrane fractions were carried out to look at tissue-specific expression, and to identify putative regulatory sites of membrane transport proteins. Around 90 unique membrane proteins were identified, which included primary and secondary transporters, ion channels and aquaporins. Primary H+ pumps from the AHA family showed little isoform specificity in their tissue expression pattern, whereas specific isoforms of the Ca2+ pump ECA/ACA family were expressed in root and shoot tissues. Several ABC transporters were detected, particularly from the MDR and PDR subfamilies, which often showed expression in either roots or shoots. Ammonium transporters were expressed in root, but not shoot, tissue. Large numbers of sugar transporters were expressed, particularly in green tissue. The occurrence of phosphorylation sites in rice transporters such as AMT1;1 and PIP2;6 agrees with those previously described in other species, pointing to conserved regulatory mechanisms. New phosphosites were found in many transporters, including H+ pumps and H+:cation antiporters, often at residues that are well conserved across gene families. Comparison of root and shoot tissue showed that phosphorylation of AMT1;1 and several further transporters may be tissue dependent. © 2008 The Authors.
    Original languageEnglish
    Pages (from-to)146-156
    Number of pages10
    JournalPlant Journal
    Volume56
    Issue number1
    DOIs
    Publication statusPublished - Oct 2008

    Keywords

    • Membrane transport
    • Oryza sativa
    • Phosphoproteomics
    • Proteomics
    • Rice

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