A scheme for the development and validation of enzyme linked immunosorbent assays (ELISA) for measurement of angiogenic biomarkers in human blood.

Garry Mcdowell; Richard Body; Cliona Kirwan; Ged Byrne; Mark Slevin

doi:10.1007/978-94-017-9716-0_35

A scheme for the development and validation of enzyme linked immunosorbent assays (ELISA) for measurement of angiogenic biomarkers in human blood.

Garry Mcdowell, Richard Body, Cliona Kirwan, Ged Byrne, Mark Slevin

Research output: Chapter in Book/Conference proceeding › Chapter › peer-review

Abstract

In this chapter the authors describe a protocol that can be applied to design and validate an ELISA technique using commercially available reagents. This often proves an economical alternative to purchasing off-the-shelf-kits. In addition, this protocol allows custom validation of the ELISA to a specific purpose. We have illustrated our protocol using VCAM as an example, however the principles and techniques described can equally be applied to all ELISA based techniques.

Original language	English
Title of host publication	Handbook of Vascular Biology Techniques
Editors	Mark Slevin, Garry McDowell
Place of Publication	Netherlands
Publisher	Springer Nature
Pages	453-463
ISBN (Electronic)	978-94-017-9716-0
ISBN (Print)	978-94-017-9715-3
DOIs	https://doi.org/10.1007/978-94-017-9716-0_35
Publication status	Published - 2015

Keywords

ELISA
Reagents
Development
Validation
VCAM
Angiogenesis markers
Serum

Research Beacons, Institutes and Platforms

Manchester Cancer Research Centre

Access to Document

10.1007/978-94-017-9716-0_35

https://books.google.co.uk/books?id=PLO1BwAAQBAJ&pg=PA453&lpg=PA453&dq=G.+McDowell,+R.+Body,+Kirwan.+C,+Byrne.+G,+Slevin.+M.+A+scheme+for+the+development+and+validation+of+enzyme+linked+immunosorbent+assays+(ELISA)+for+measurement+of+angiogenic+biomarkers+in+human+blood.+In+Handbook+of+Vascular+Biology+Techniques&source=bl&ots=6N_5ZBauGr&sig=DbtS-htcmgpN5jPv0X04Hiz6Ehw&hl=en&sa=X&ved=0ahUKEwj_mIf6nNbSAhUGQpAKHTxvC1IQ6AEIGjAA#v=onepage&q=G.%20McDowell%2C%20R.%20Body%2C%20Kirwan.%20C%2C%20Byrne.%20G%2C%20Slevin.%20M.%20A%20scheme%20for%20the%20development%20and%20validation%20of%20enzyme%20linked%20immunosorbent%20assays%20(ELISA)%20for%20measurement%20of%20angiogenic%20biomarkers%20in%20human%20blood.%20In%20Handbook%20of%20Vascular%20Biology%20Techniques&f=false

Cite this

@inbook{4e5250c817bc406f833219f77f36832b,

title = "A scheme for the development and validation of enzyme linked immunosorbent assays (ELISA) for measurement of angiogenic biomarkers in human blood.",

abstract = "In this chapter the authors describe a protocol that can be applied to design and validate an ELISA technique using commercially available reagents. This often proves an economical alternative to purchasing off-the-shelf-kits. In addition, this protocol allows custom validation of the ELISA to a specific purpose. We have illustrated our protocol using VCAM as an example, however the principles and techniques described can equally be applied to all ELISA based techniques.",

keywords = "ELISA, Reagents, Development, Validation, VCAM, Angiogenesis markers, Serum",

author = "Garry Mcdowell and Richard Body and Cliona Kirwan and Ged Byrne and Mark Slevin",

year = "2015",

doi = "10.1007/978-94-017-9716-0_35",

language = "English",

isbn = "978-94-017-9715-3",

pages = "453--463",

editor = "Mark Slevin and Garry McDowell",

booktitle = "Handbook of Vascular Biology Techniques",

publisher = "Springer Nature",

address = "United States",

}

A scheme for the development and validation of enzyme linked immunosorbent assays (ELISA) for measurement of angiogenic biomarkers in human blood. / Mcdowell, Garry; Body, Richard ; Kirwan, Cliona et al.
Handbook of Vascular Biology Techniques. ed. / Mark Slevin; Garry McDowell. Netherlands: Springer Nature, 2015. p. 453-463.

Research output: Chapter in Book/Conference proceeding › Chapter › peer-review

TY - CHAP

T1 - A scheme for the development and validation of enzyme linked immunosorbent assays (ELISA) for measurement of angiogenic biomarkers in human blood.

AU - Mcdowell, Garry

AU - Body, Richard

AU - Kirwan, Cliona

AU - Byrne, Ged

AU - Slevin, Mark

PY - 2015

Y1 - 2015

N2 - In this chapter the authors describe a protocol that can be applied to design and validate an ELISA technique using commercially available reagents. This often proves an economical alternative to purchasing off-the-shelf-kits. In addition, this protocol allows custom validation of the ELISA to a specific purpose. We have illustrated our protocol using VCAM as an example, however the principles and techniques described can equally be applied to all ELISA based techniques.

AB - In this chapter the authors describe a protocol that can be applied to design and validate an ELISA technique using commercially available reagents. This often proves an economical alternative to purchasing off-the-shelf-kits. In addition, this protocol allows custom validation of the ELISA to a specific purpose. We have illustrated our protocol using VCAM as an example, however the principles and techniques described can equally be applied to all ELISA based techniques.

KW - ELISA

KW - Reagents

KW - Development

KW - Validation

KW - VCAM

KW - Angiogenesis markers

KW - Serum

U2 - 10.1007/978-94-017-9716-0_35

DO - 10.1007/978-94-017-9716-0_35

M3 - Chapter

SN - 978-94-017-9715-3

SP - 453

EP - 463

BT - Handbook of Vascular Biology Techniques

A2 - Slevin, Mark

A2 - McDowell, Garry

PB - Springer Nature

CY - Netherlands

ER -

A scheme for the development and validation of enzyme linked immunosorbent assays (ELISA) for measurement of angiogenic biomarkers in human blood.

Abstract

Keywords

Research Beacons, Institutes and Platforms

Access to Document

Fingerprint

Cite this